EMAGE mouse gene expression spatial database

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EMAGE:21566

Clec7a C-type lectin domain family 7, member a ( MGI:1861431)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001079. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001079_01	euxassay_001079_02	euxassay_001079_03	euxassay_001079_04

euxassay_001079_05	euxassay_001079_06	euxassay_001079_07	euxassay_001079_08	euxassay_001079_09

euxassay_001079_10	euxassay_001079_11	euxassay_001079_12	euxassay_001079_13	euxassay_001079_14

euxassay_001079_15	euxassay_001079_16	euxassay_001079_17	euxassay_001079_18	euxassay_001079_19

euxassay_001079_20	euxassay_001079_21	euxassay_001079_22	euxassay_001079_23	euxassay_001079_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21566_wholemount_strong.wlz

21566_wholemount_moderate.wlz

21566_wholemount_weak.wlz

21566_wholemount_possible.wlz

21566_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21566_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4

vertebral axis musculature

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

ventral grey horn

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8

stomach

moderate

homogeneous

moderate expression: see section 3 4 5 6 7 8 9 0 1 2

hindgut

moderate

homogeneous

moderate expression: see section 5 6

midgut

moderate

homogeneous

moderate expression: see section 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4722
Entity Detected:	Clec7a, C-type lectin domain family 7, member a ( MGI:1861431)
Sequence:	sense strand is shown >T4722 CCACGCGTCCGCTTAGTGAGCCTCATCCTGGCAGTTATTTTACTAGTAAAGAACATTCAAGTGCTCTGCC TACCTAGGGCCCTGTGAAGCAATGAAATATCACTCTCATATAGAGAATCTGGATGAAGATGGATATACTC AATTAGACTTCAGCACTCAAGACATCCATAAAAGGCCCAGGGGATCAGAGAAAGGAAGCCGGGCTCCATC TTCACCTTGGAGGCCCATTGCAGTGGGTTTAGGAATCCTGTGCTTTGTGGTAGTAGTGGTTGCTGCAGTG CTAGGTGCCCTAGCATTTTGGAGACACAATTCAGGGAGAAATCCAGAAGAGAAAGACAACTTCCCATCAA GAAATAAAGAGAACCACAAGCCCACAGAATCATCTTTAGATGAGAAGGTGGCTCCCTCCAAGGCATCCCA AACTACAGGAGGTTTTTCTCAGCCTTGCCTTCCTAATTGGACCATGCATGGGAAGAGCTGTTACCTATTT AGCTTCTCAGGAAATTCCTGGTATGGAAGTAAGAGACACTGCTCCCAGCTAGGTGCTCATCTACTGAAGA TAGACAACTCAAAAGAATTTGAGTTCATTGAAAGCCAAACATCATCTCACCATATTACTGCATTTTGGAT AGGCCTTTCCCGCAATCAGAGTGAAGGGCCATGGTTCTGGGAGGATGGATCAGCATTCTTCCCCAACTCG TTTCAAGTCAGAAATACAGCTCCCCAGGAAAGCTTACTGCACAATTGTGTATGGATTCATGGATCAGAGG TCTACAACCAAATCTGCAATACTTCTTCATACAGTATCTGTGAGAAGGAACTGTAAATGTATGTGAGAAT ATAAAGATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTACATGCACACACACCACCACCACCACCACTACC AACAACAGAACAGAACAGAACAGAACAGAACAGAACAGATTAATATTAAAAAACAGAGAAAATGCTGGGA TGCTAAGAGACTTTAACCTCAGTTGAGAACTTGGATGAAGAAGCTGAGACTTTTGTACTTGTCATCTTCA CAAAGATGGTGGCACTATCTTCCAGTTAGGAAGTCACTAGACATGGAGTGAGGGCAGCTCAAAAATACAG AGAATATGTGAACCTGAGGTACCCTGACTCAAATTTCACAACCACAATGAAACCCCTACACTATCAGGAA ACACTGTAGAGGAGTGAGACTGAAGACTTTAAAAGCCAGAGAATCAGCCTACTTACTGTGGTGTTTTCTA GACAGGACAGGGAAAGTATATCTAGGAAATAAAAACAATACAATTCAGCAAACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5036647 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5036647 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001079 same experiment
	EMAGE:21568 same embryo
	EMAGE:21563 same embryo
	EMAGE:21565 same embryo
	EMAGE:21567 same embryo
	EMAGE:21564 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source