EMAGE mouse gene expression spatial database

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EMAGE:21568

Rcsd1 RCSD domain containing 1 ( MGI:2676394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001089. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001089_01	euxassay_001089_02	euxassay_001089_03	euxassay_001089_04

euxassay_001089_05	euxassay_001089_06	euxassay_001089_07	euxassay_001089_08	euxassay_001089_09

euxassay_001089_10	euxassay_001089_11	euxassay_001089_12	euxassay_001089_13	euxassay_001089_14

euxassay_001089_15	euxassay_001089_16	euxassay_001089_17	euxassay_001089_18	euxassay_001089_19

euxassay_001089_20	euxassay_001089_21	euxassay_001089_22	euxassay_001089_23	euxassay_001089_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21568_wholemount_strong.wlz

21568_wholemount_moderate.wlz

21568_wholemount_weak.wlz

21568_wholemount_possible.wlz

21568_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21568_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 2 3 4 5 6 7

foregut-midgut junction

weak

homogeneous

weak expression: see section 4 5 6 7 8 9

esophagus

weak

regional

weak expression: see section 3 4 5

stomach

weak

homogeneous

weak expression: see section 3 4 5 6 7 8 9 0 1 2

hindgut

weak

homogeneous

weak expression: see section 4 5 6 7

midgut

weak

homogeneous

weak expression: see section 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1

kidney calyx

weak

homogeneous

weak expression: see section 9 0 1 2 8 9 0 1 2 3

kidney pelvis

weak

homogeneous

weak expression: see section 1 0

chondrocranium

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4728
Entity Detected:	Rcsd1, RCSD domain containing 1 ( MGI:2676394)
Sequence:	sense strand is shown >T4728 TGCCCGCTACAGCCCCGAAAGTGACCCGGGCTGGGAGCCGAGGGGACAGCGGGGATCGTGGGCTCTTAAC CCGAGAGAGCGGGCGTGTCCGCAGCCGGAGGAAGACTACCTGGAGGACATGGAGGAAAGACCCTCAGAGA CCAACTCCAATGTTGACAGCTCAGCGCAGCCTTCAGTGGCCCAGCTGGCTGGGCGCTTTCGGGAGCATGC AGCTGTGGCCAGGGAGACACCAGCCAGTAAGCCAACAAGAAGGAAACCACCCTGCTCCCTCCCCCTGTTT CCCCCCAAGGTAGAGCTGGGCCAGAACGGTGAGGAGAAATCACCATCCGGCGCCAGCCACCCACCTAAAA TCAAGGTGAAGAGCTCACCCCTGATCGAGAAGCTTCAGGCCAACTTAGCCTTTGACCCGGCAGCTCTTCT GCCTGGGGCTTCACCCAAAAGTCCTGGACTCAAGGCCATCATATCACCATTTCACAGTCCCCCTTCCACA CCCAGTAGCCCCGGCATCCGATCCCACCCAAGTGAGGCAGAAGAGGTGCCTGTGAGCTTTGACCAGCCTC CGGAAGGAACTCACCTGCCCTCTTACAATAAGGTGCGGACTAGAGGCTCAATAAAAAGACGTCCTCCCTC GCGGCGATTCCGAAGGTCTCAGTCGGACTGTGGGGATTTTAGAGATTACAGGGCTGTGGAGCCATCTCAG GAAAACGGTGCCAGGGAAGAGAATGGGGATGACGTGTTTCCTAGCAAGAGCAAGGACCCCGGGTCCCCTC AACTCAACCAGGAGGCTATGGCAGACGGGGTGGAGGGAACTCCGCGGTCTGCAGAAAAGCCACGGAGAAG GAACACGTGTAACAGCACAGAGAAGCCAGAGGAGCTGGTCAGGACCCCAGAGGAGGCGAATGCTGGAGAG AAGGTTGGACAGAATCCAGACACAGCTAGTCAGGGTCATCCAGAGGTCCAGGCGCCATCGCAAACCGGCA GCCCAGAGGCTGAGAATGGGTGCGGAAGCCCACGGGAAGAGACAGCCCCGGGAGAGCATACAGACACTGG GAAGGCCACTGAAGGGACAGCCTCTGAGGAGAGGGTAGCAGATGAAGATAGGCTCGGACAGAAAAGCCCA GACGCAAATATGCCTGAGGAGGAGGGAGTGGTCAGGGAGAAAGCCCCACAAACCTCTTCTGGAAAAGCAG AAGGTACTACTATCGCAGAGCCGGATACAAAGCAAAAGGAGGAGGCGCCTCTGGAGCCAAGCTGTAGCCC AGGGGCCGACCATGCTGCGGGAGAGATCACCAGTGAGATCCAGAATGAGAAAGCAGTCTCCATGGATGAC ATCCCCATCGAGGATACCCGAATGTGATGAAGGGCTCAGGGTACCAGTGATGGGGCCACTGGAGACATCT CCTTGGAAACAGCAGCAGTAACAGCAGCAGCAGCAGTGCATGAAGCCACTGCAGAAGCAGAATACACCAA GCATCTGGAGACAGCCCAGGATGCAGGGTGGATGGTCCCCCGTAGGCTACACCAACCGTCTCCTCACTGG AGATGAGAGCTGAGGACAGTAGAGTTTTATCAACTTGAGTTTGTGTCACCTGACGCACGACTCAAAGTTA AATATATTTAAAGCAATTTGGTGTGGTGTCTGCTCCACGACCTTCCTGTTGATCCCCAAGTCTTCCTGCA GCCAACCTCCCAACTAGGCTCTTGTTGAACTTGTGTAAAGTTATGGCCTTTTTCTATCGTTCTAATGTTT TTATGGTTCTCAGACGTAACTTAAAACTAATTTTAGGTAGATAAAGAAGGTAGTCTTGTGGAGAATAAGA TTCCCTAAGTTACCGAGACCTGAAAACATACCAAAGCCCTCACGACGACCGTTCTGCTTGCTCCAGATGG GAATCAGCTGACCAGTTTAAAAAACTCAAAGAGATATTTATTAAAACATTTAAAGTCGTGTTCCTGCCTG CTCCAAGGACAGGAACTTTTCCCTGTCCCACACAGCCTGGCTGATGAGAACAAGGGGAGGTGATTGATCC ATCTCATCCCATCCAAAGGAGAGGCAGGATTCATCCCCTCCACCCCTCAAGGTGCCACCATGTGCCTGCC TTGCTCTGAGCATGGGTTGTACTCTCCTGGAAGGTTACACTTGAACAGGAAACTGGCCGGCCTCTGGGAG GGACGTTCAGTGTAGGCAGTACAGCTTACCTTCTGAGAGCGTCTCTCTTCACTTCTCTATGAAGTTTTGT TTGTTTGCTTTTGTTTTTGTTTTTTCAAATCTCACCATGTAGACTTATATGTAGCCCAGGCTGGTTTTGA ATTAGTGATCCCCCTGCCTCTGCCTCTAGATTGTGGGTATGCATCACTGTGCCTGGTTCTCAGTAAGCAT TTCTCAACTCCAAGTTCTTCTCCCGGACTCAGGAGGCATGAGTTAGGGTCTCCAGCCTGTTTTCATCAGG AACTCCTGCTCAGTATCCTGTCCGGGGATAAAACAGGGTTTGCAGGAATATGGGTCGGTCTGATATCCAT TCCTCTGATCACCCTCCCCTCCATGAACTCCTGCTGAAGAAAGGTTAACATAAATAAATGATGAATCTTC AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5037953 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5037953 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001089 same experiment
	EMAGE:21566 same embryo
	EMAGE:21563 same embryo
	EMAGE:21565 same embryo
	EMAGE:21567 same embryo
	EMAGE:21564 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source