EMAGE mouse gene expression spatial database

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EMAGE:21575

4931406C07Rik RIKEN cDNA 4931406C07 gene ( MGI:1918234)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003185_01	euxassay_003185_02	euxassay_003185_03	euxassay_003185_04	euxassay_003185_05

euxassay_003185_06	euxassay_003185_07	euxassay_003185_08	euxassay_003185_09	euxassay_003185_10

euxassay_003185_11	euxassay_003185_12	euxassay_003185_13	euxassay_003185_14	euxassay_003185_15

euxassay_003185_16	euxassay_003185_17	euxassay_003185_18	euxassay_003185_19	euxassay_003185_20

euxassay_003185_21	euxassay_003185_22	euxassay_003185_23	euxassay_003185_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21575_wholemount_strong.wlz

21575_wholemount_moderate.wlz

21575_wholemount_weak.wlz

21575_wholemount_possible.wlz

21575_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21575_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

homogeneous

moderate expression: see section 7 8 9 0 1 2 3 4 5 6 7 8 9

telencephalon mantle layer

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

telencephalon marginal layer

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 1 2 3 4 6 7 8 9

hindbrain

moderate

homogeneous

moderate expression: see section 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

midbrain

moderate

homogeneous

moderate expression: see section 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

facial vii ganglion

moderate

regional

moderate expression: see section 2

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 8 9 0

trigeminal v ganglion

moderate

regional

moderate expression: see section 4 5 6 7 8 9 0 9 0 1 2 3 4

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 8 9 0

spinal cord

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0

dorsal root ganglion

moderate

regional

moderate expression: see section 1 2 3 4 8 9 0 1

not examined

regional

not examined expression: see section 1 2 3 4

neural retina

moderate

regional

moderate expression: see section 1 2 3 4

nucleus pulposus

weak

regional

weak expression: see section 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4198
Entity Detected:	4931406C07Rik, RIKEN cDNA 4931406C07 gene ( MGI:1918234)
Sequence:	sense strand is shown >T4198 CTTTGCCTTCATCAAGTAACTGAGCTCCACCATTCATTTCCAGAGCCGGGACTGTTCCTGCTGGTGCCCC GTGAGCTCTCGTGGTGGTCAGCCAACAGCTGCGGTGTCTGAGCAGCCTTGCTGAGAGGAAGGAGAATGGC TTGCAGTGAGTTTTCCTTTCACATGCCAAGTCTGGAGGAGCTTGCTGAAGTTTTGCAGAAGGGGCTAACT GACAACTTTGCTGATGTCCAGGTCTCAGTGGTTGACTGCCCAGATTTAACAAAGGAGCCATTTACCTTTC CTGTAAGAGGCATCTGTGGGCAAACTAGAATTGCAGAAGTAGGAGGTGTGCCTTACTTATTGCCTCTTGT AAACAAAAAAAAAGTTTATGACCTAAATGAAATTGCAAAAGTAATAAAGCTGCCCGGAGCTTTTATCCTT GGAGCAGGGGCAGGTCCATTTCAGACGCTTGGGTTCAATTCTGAGTTCATGCCAATTGTTCAAACAGCAA GTGAACACAACCAACCTGTGAATGGAAGTTACTTTGCCCATAAAAACCCTGCAGATGGAGCGTGCCTGCT GGAGAAATACAGCCAAAAATATCATGATTTTGGATGTGCACTACTGGCTAATCTTTTTGCCAGTGAGGGC CAACCTGGCAAGGTCATTGAAGTGCAAGCCAAGAGAAGAACAGGAGAACTTAACTTTGTGAGCTGCATGA GACAGACACTGGAGGAGCACTATGGTGACAAGCCTGTGGGGATGGGAGGCACCTTCATTGTGCAGAAGGG GAAAGTGAAAGCCCACATCATGCCTGCAGAGTTTTCTTCCTGCCCACTGAACTCGGACGAAGCTGTCAAT AAATGGCTACACTTCTATGAGATGAAGGCTCCCTTGGTTTGTCTACCAGTTTTTGTTTCCAAAGACCCTG GACTTGATTTGCGACTGGAGCACACACATTTCTTCAGTCATCATGGAGAAGGTGGACACTACCACTACGA TACGACCCCAGACACAGTGGAGTACCTTGGATACTTCTCACCTGCACAGTTCCTCTATCGCATTGATCAG CCCAAAGAGACCCATGCCTTTGGGAGAGATTAAAGCAGCTGTGTAATGGTTAGCTAAGGTTAAGTTACTA ACCCAGCAGCTGATAGTAATTCAAAAATACAGTAGAGGACCAGAGAGGGGCGGGGGGGGGGGCGGGGAGA AAGAGAGGAAGAGGAAGAGAGAGAGAGAGAGAGAGGCATTCCCTAGCAAGCAGCCAAGGCCCTACCTTAA TCCCCAGCACAGCTAATAAAATTAATCACTACACAAACACTATTTTTATGGCATTTGAAATGCAAGGATG TGGACACATGACACTCATATTACCATATTATTTAACATCTTCATGTGAGGGGAGCGTATTGGGAACATAG ATAGCTAATTGAGATGCTCACTTTAGTAAACCAATCTTCTGTCTTTATAGCCCAGCAAAGTCTACCTTCT CTTCATACCACAGATATGAATATGGACAGGGACCTTCCATTATTCCTGCTATCTAGGAATAACTTACATT TGGGTGGCATTACTAAATTTTTCAAGTTCATCTCACAAGGGCTAAGGGAGTATTAGACACAACAGTGTGG GATCTTTGCACTGAGTTTTAAAAGCCCACTGTGGAAATCAGTGCACTACGTTCTCAGCACTCAGGACTAC CCACACTCAGAAGTCTGCAGTTGCCTTCCACAGATGTTAGTAGCATTGATGAGCCAGGCTCCGCCTGAAT TAGGTCTCTACCCAACAGTTTGTACCTTCGTAATACTGTTTGCAAGATAAGAAAGAAAAGAAACAGCAAC AAAACACAACACCTAACATGGCCACTCCCCATCATGAGATATAAAACTACCCTCTTATTATGTGGTGTCT TAAAGAGACACTTTTGTGCCCTTTCTTTGGCTATATAGACTGTTGAGACAGTGAACCACAAGAAATTGGA ATTCCCTCAGGAATTTCATGGCAGGAGAAGGAAAAGGCTGAAGCCTGGTGCCAGCCAGAGGAGGAACTTG TCTTCTGGAAAGATGTCACGTACCTCTCAGGACAAACAGGAACCCAGACAACAGTGGTGGGCAGGACCAC AGCTTGATCTGCTTAGTTAGGTATACCTCGTGCCTTCTATTTAAACCTGAACCTCATGCCAGGACCCAGC ATGAATTCTGCAGGATGGTGGGGGAGGGGTGGGAATCCAGAGACCTGTTTATTCCTCTTCCCAAAGTGAC TACATTCAAGGAATCTTTTTGTGCTTTTCAAAGGATATTACACTATTACTGAGCGCTGATGGCTAGCATA TGGTGATGATGGTTAGAAAACTCCTTGCTGTTCTGTGTCATAGAACCTGTGATGATCATTAGCCAGAAAT CAACTTTCCAACCTTCCCAATGGCAATAAGATGTGGGTCTAGGAACTCTTTAGAACTAAGCCCTAAACGT TTTCTGATTCTTTACTTTGTAATGCACATGTCTTGTATTATTTTTAATAATAGTGTTATTTTTAATTAAA CATGATTTTTGAGGTGAAACTGAATAAAATCACTTAATACTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4489072 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4489072 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003185 same experiment
	EMAGE:21580 same embryo
	EMAGE:21578 same embryo
	EMAGE:21579 same embryo
	EMAGE:21577 same embryo
	EMAGE:21576 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source