EMAGE mouse gene expression spatial database

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EMAGE:21576

Pik3r1 phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) ( MGI:97583)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003253_01	euxassay_003253_02	euxassay_003253_03	euxassay_003253_04	euxassay_003253_05

euxassay_003253_06	euxassay_003253_07	euxassay_003253_08	euxassay_003253_09	euxassay_003253_10

euxassay_003253_11	euxassay_003253_12	euxassay_003253_13	euxassay_003253_14	euxassay_003253_15

euxassay_003253_16	euxassay_003253_17	euxassay_003253_18	euxassay_003253_19	euxassay_003253_20

euxassay_003253_21	euxassay_003253_22	euxassay_003253_23	euxassay_003253_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21576_wholemount_strong.wlz

21576_wholemount_moderate.wlz

21576_wholemount_weak.wlz

21576_wholemount_possible.wlz

21576_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21576_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 4 weak expression: see section 7 8 9 2 3

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 1 2 7 8

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 5 6 7 8 9 0 1 2 3 4

midbrain ventricular layer

moderate

regional

moderate expression: see section 0 1 4 5 7 weak expression: see section 8 9 2 3 6

lower jaw incisor

moderate

regional

moderate expression: see section 3 4 7 8

upper jaw incisor

moderate

regional

moderate expression: see section 3 4 7 8

liver lobe

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 1 2 weak expression: see section 9 0 1 2 3 4 5 6 7 8 9 0 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4702
Entity Detected:	Pik3r1, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) ( MGI:97583)
Sequence:	sense strand is shown >T4702 CTTTTCTTATAACCGAGCAAAGCCAAGGAAACTGTCGCACACATGTACACCACGGTTTGGACTATGGAAG ACCTGGACTTAGAGTGTGCCAAGACAGATATAAACTGTGGCACAGACTTGGTGTTTTATATAGAAATGGA TCCACCAGCACTGCCCCCCAAACCACCCAAGCCCACTACTGTAGCCAACAACAGCATGAACAACAATATG TCCTTGCAGGATGCTGAATGGTACTGGGGAGACATCTCAAGGGAAGAAGTGAATGAAAAACTCCGAGACA CTGCTGATGGGACCTTTTTGGTACGAGACGCATCTACTAAAATGCACGGCGATTACACTCTTACACTAAG GAAAGGAGGAAATAACAAATTAATCAAAATCTTTCACCGTGATGGAAAATATGGCTTCTCTGATCCATTA ACCTTCAACTCTGTGGTTGAGTTAATAAACCACTACCGGAATGAGTCTTTAGCTCAGTACAACCCCAAGC TGGATGTGAAGTTGCTCTACCCAGTGTCCAAATACCAGCAGGATCAAGTTGTCAAAGAAGATAATATTGA AGCTGTAGGGAAAAAATTACATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATATGATAGATTA TATGAGGAGTACACCCGTACTTCCCAGGAAATCCAAATGAAAAGAACGGCTATCGAAGCATTTAATGAAA CCATAAAAATATTTGAAGAACAATGCCAAACCCAGGAGCGGTACAGCAAAGAATACATAGAGAAGTTTAA ACGCGAAGGCAACGAGAAAGAAATTCAAAGGATTATGCATAACCATGATAAGCTGAAGTCGCGTATCAGT GAGATCATTGACAGTAGGAGGAGGTTGGAAGAAGACTTGAAGAAGCAGGCAGCTGAGTACCGAGAGATCG ACAAACGCATGAACAGTATTAAGCCGGACCTCATCCAGTTGAGAAAGACAAGAGACCAATACTTGATGTG GCTGACGCAGAAAGGTGTGCGGCAGAAGAAGCTGAACGAGTGGCTGGGGAATGAAAATACCGAAGATCAA TACTCCCTGGTAGAAGATGATGAGGATTTGCCCCACCATGACGAGAAGACGTGGAATGTCGGGAGCAGCA ACCGAAACAAAGCGGAGAACCTATTGCGAGGGAAGCGAGACGGCACTTTCCTTGTCCGGGAGAGCAGTAA GCAGGGCTGCTATGCCTGCTCCGTAGTGGTAGACGGCGAAGTCAAGCATTGCGTCATTAACAAGACTGCC ACCGGCTATGGCTTTGCCGAGCCCTACAACCTGTACAGCTCCCTGAAGGAGCTGGTGCTACATTATCAAC ACACCTCCCTCGTGCAGCACAATGACTCCCTCAATGTCACACTAGCATACCCAGTATATGCACAACAGAG GCGATGAAGCGCTGCCCTCGGATCCAGTTCCTCACCTTCAAGCCACCCAAGGCCTCTGAGAAGCAAAGGG CTCCTCTCCAGCCCGACCTGTGAACTGAGCTGCAGAAATGAAGCCGGCTGTCTGCACATGGGACTAGAGC TTTCTTGGACAAAAAGAAGTCGGGGAAGACACGCAGCCTCGGACTGTTGGATGACCAGACGTTTCTAACC TTATCCTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTT TCTTTCTAATTTAAAGCCACAACACACAACCAACACACAGAGAGAAAGAAATGCAAAAATCTCTCCGTGC AGGGACAAAGAGGCCTTTAACCATGGTGCTTGTTAACGCTTTCTGAAGCTTTACCAGCTACAAGTTGGGA CTTTGGAGACCAGAAGGTAGACAGGGCCGAAGAGCCTGCGCCTGGGGCCGCTTGGTCCAGCCTGGTGTAG CCTGGGTGTCGCTGGGTGTGGTGAACCCAGACACATCACACTGTGGATTATTTCCTTTTTAAAAGAGCGA ATGATATGTATCAGAGAGCCGCGTCTGCTCACGCAGGACACTTTGAGAGAACATTGATGCAGTCTGTTCG GAGGAAAAATGAAACACCAGAAAACGTTTTTGTTTAAACTTATCAAGTCAGCAACCAACAACCCACCAAC AGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5031261 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5031261 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003253 same experiment
	EMAGE:21580 same embryo
	EMAGE:21575 same embryo
	EMAGE:21578 same embryo
	EMAGE:21579 same embryo
	EMAGE:21577 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source