EMAGE mouse gene expression spatial database

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EMAGE:21579

Gpt2 glutamic pyruvate transaminase (alanine aminotransferase) 2 ( MGI:1915391)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003194_01	euxassay_003194_02	euxassay_003194_03	euxassay_003194_04	euxassay_003194_05

euxassay_003194_06	euxassay_003194_07	euxassay_003194_08	euxassay_003194_09	euxassay_003194_10

euxassay_003194_11	euxassay_003194_12	euxassay_003194_13	euxassay_003194_14	euxassay_003194_15

euxassay_003194_16	euxassay_003194_17	euxassay_003194_18	euxassay_003194_19	euxassay_003194_20

euxassay_003194_21	euxassay_003194_22	euxassay_003194_23	euxassay_003194_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21579_wholemount_strong.wlz

21579_wholemount_moderate.wlz

21579_wholemount_weak.wlz

21579_wholemount_possible.wlz

21579_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21579_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 9 0 1 2 3 4 weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 6 7 8

hypothalamus ventricular layer

strong

regional

strong expression: see section 1 moderate expression: see section 2 3 4 5

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 1 moderate expression: see section 2 3 4

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 1 2 6 7

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 1 2 3 4 5 6 7 moderate expression: see section 8 9 0 1 2 4 5 6 7 8 9 0 1 2 3 4

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 0 1 2 3 4 5 6

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 0 1 2 3 4 5 6

pons ventricular layer

strong

regional

strong expression: see section 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

midbrain ventricular layer

strong

regional

strong expression: see section 8 9 0 1 3 4 5 6 7

spinal cord ventricular layer

strong

regional

strong expression: see section 2 3 4 5 6 7

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 9 0 1 2 3 6 7 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4838
Entity Detected:	Gpt2, glutamic pyruvate transaminase (alanine aminotransferase) 2 ( MGI:1915391)
Sequence:	sense strand is shown >T4838 GGAGGTGCTGGTGCCAGGCGCCAGGATGCGGTGGCCGGCCACCGGGTTTGGGAGCAGCCCAGGCTCACCT TAACCGGAGCGGTGCGGACGGTCCCGCGGCGACAGGGCTAATCTCGGCAGGTTCGCGATGCAGCGGGCAG CGGTGCTGGTGCGGCGGGGCTCCTGCCCCCGCGCCTCGGGCCCCTGGGGCCGTAGTCACAGCAGCGCTGC AGCCGAAGCCTCGGCGGCGCTCAAGGTGCGACCGGAGCGCAGCCCTCGAGACCGCATCCTCACCCTGGAG TCCATGAACCCGCAGGTGAAGGCGGTGGAGTACGCTGTGCGGGGACCCATCGTGCTCAAAGCCGGCGAGA TCGAGATGGAGCTGCAGCGGGGTATCAAAAAACCATTCACTGAGGTAATCCGAGCCAACATTGGGGATGC CCATGCTATGGGCCAGCAGCCAATCACCTTCCTCCGTCAGGTGATGGCACTCTGCACCTACCCAAACCTA CTAAACAGCCCCAGCTTCCCAGAAGACGCTAAGAAACGAGCGCGGCGGATCCTGCAGGCTTGTGGTGGAA ACAGCTTGGGATCTTACAGTGCTAGCCAGGGCGTTAACTGTATCCGTGAAGATGTGGCAGCCTTTATCAC CAGGAGAGATGGTGTGCCTGCAGACCCAGACAACATTTACCTGACTACTGGAGCTAGCGACGGTATTTCT ACAATCCTGAAACTCCTGGTCTCCGGTGGTGGCAAGTCACGGACCGGCGTGATGATTCCCATCCCCCAGT ATCCCTTGTACTCCGCGGTCATCTCCGAGCTCGACGCGGTGCAGGTCAACTACTATCTGGATGAAGAGAA CTGCTGGGCTTTGAATGTGGACGAGCTCCGGCGGGCATTGTGGCAAGCCAAAGACCACTGTGACCCTAAA GTTCTCTGCATTATCAACCCCGGAAACCCCACAGGCCAGGTACAAAGCAGAAAGTGCATAGAAGATGTGA TTCACTTTGCCTGGGAAGAGAAGCTTTTTCTCCTGGCTGATGAGGTGTACCAGGACAACGTGTACTCTCC AGACTGCAGATTCCACTCCTTTAAGAAAGTGCTTTACCAGATGGGGCACGAGTACTCCAGCAACGTGGAG CTCGCCTCCTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGTGGCTACAGAGGGGGCTACATGGAGG TGATCAATTTGCACCCCGAGATCAAAGGCCAGCTGGTGAAGCTACTCTCGGTTCGCCTCTGTCCGCCAGT GTCAGGACAGGCCGCCATGGACATTGTTGTGAATCCACCAGAACCAGGAGAGGAGTCCTTTGAGCAATTC AGCAGGGAAAAAGAATTCGTCCTTGGTAATCTGGCCAAAAAAGCAAAGCTGACAGAAGATCTGTTTAACC AAGTCCCAGGGATCCAGTGCAACCCCTTGCAAGGAGCTATGTATGCGTTCCCTCGGATTCTCATCCCTGC CAAGGCCGTGGAGGCAGCTCAGTCCCATAAAATGGCTCCAGACATGTTCTACTGCATGAAGCTCCTGGAG GAGACTGGCATCTGTGTCGTGCCTGGCAGTGGCTTTGGGCAGCGAGAAGGCACTTACCACTTCAGAATGA CCATTCTCCCTCCGGTGGATAAACTGAAGACCGTGCTCCACAAGGTGAAAGACTTTCACCTGAAGTTCCT GGAGCAGTACTCATGAGGACGCCTCAGGCACCGGAGCCAGACCCTCCCAAGACCACCCAGGCCTTCCTCA AGGACTCTGCCTCAGACCTCAGACAGGCCACCAACGCTGTTCATCTTCATTTCCCCAAGGAGACTTCTTT CTTTGTGCCTTGATGTTTGAGAGTTCTTCGAGCAAACAGTGGTTTTGCAATGTCTCACAGGCCCTGTTTT TGTTTTGTTTTGTTTTGTTCTTTTTTTAAATGCAACCAAAGTAGAGTCAACCTGCTCGGCAGATGTACTT GGATTCTCTGAATCGCTATTCTGTTTGGAGAGTTCCTTTGGGTTTTAAGCAGCCAGAGTACATGGAAATG AGATTATGTCAGATCTGGAGAAACAAGCAGGTGTTGGGAAATATGTGACTTGACATGATAAGGGCTGGGA ATCCAGAAATCAATAGTGAGATCCATGAAATCAAACCCTGACCAGTGTGGAAATGTAGCCTTTTGGACAG TAAGCCTGCAAGTCTAGTGAGAACTCAGAGAAAGCTGACCATTCTGGTCTGAAGATAGGCAGCGCATCAC AGGCAAGAATATCGAAGTCAGTAGTAGGACAGGGGTCACATCAGATACCAGCTCAAATTGCACTAGCTAT CTAGAACAGTTTTCTCCAGGTTTGCCTGAGCCTTGATGCATACCATCGCCCTCTGCTGGTCGCAGCAGAG ATAAGCAAGGGCTGAAAATGGAGGCAATCCTTTCCCAAGGCCCTGAAAGTTGTTTTTCATGGTTTCAAAC TGAATTTGGCTCATTTGTAACTAACTGATCACGGTGCCTGGTTACACTGGCTGCCAAGAAGGAGGCATGC AATCTGATTCAGTGCTCTCTTCACATCAGTTTCCTGCCTCCCTCCCTCATCTGCGGACAGCATCCTATCT CATCAGGCTTCCCTGTGTGTCACAAAGTAGCAGCCACCAAGCAAATATATTCCTTGAATTAGCACACCTG GGTGGGCCATGTGCGCACCAAGGAAACAGGTGCTATAGGGAGCGCCAGGTCAGGCTTGTCTCTTAACTGT CTCCGTTCTTCAGTGAGCGTGGGAAAGCTGTCGGGAGCTCCCGCGCAGGAGCCTGGGTACCCACGCAGTG AGTCAAGGGAGTTTTCGGAGCCAGAGAGAGAACGATGTGAAGGCTGTGGAGTAAGGCTGAAACCAGCTTC CTGCCCTATAGTCCCACACTGCAGGGGGTGCGACTTTAAAACAGAACTTCAAGTTGTTAACACTCACAAG CATTGCATTACTGTGAAGGAAGTAGCCGCATCCATAATAGGATGTGACGGTCTACAGCTTTTCCTTTAAA AGCTGAAAAGGTACCATGTGTGCTCGCTAGGCATATAATCCAGATATGCTCCAGAGTTCTGAGATTCTTC CATGAAAGGTTAACTAGAAGCTAGAATATTTTTTTATATTTTTGTAACAATTGGCTTTTTTCATGGGGGG AGGGGAGTAGAGGGTTAGTATTTATAGTCCTAACAAGTCCAAAAATTTTTATAAGTGTCTTCAGATTATA AATAACCCTCCAAATTTTGCAATGTTTACATGTTTTTTTTTTAAGATGACAAATATGCTTGATTTGCTTT TTAAATAAAAGTTTAGCTGTTCTAAGAGATTAACTTCAAGTAGGATGGCTGGTTATGATAGTTTGGATTT TCTACAGGTTCTGTTGCCATGCCTTTTGGGTTTCAGCATCACTCGAGCCGCAGCATGTGGGTGGGGCTGT GGAAACCTGGCCAGGCTGGACCTGGTCAGCCACACCTCAGAGACATTGTTTCCATTTGGATGTGAGCAGG CGCAGGCCTGCATGCTCTTTCCTACTTAGCATCATCAGTTCTTCCGCCTCCTTAGCATGGTTCTTTGTAA CAGCCATGCTGGGAAGCTCTGAACAATAAAATACTTCCAGAGTGGTGAAAAAAAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065322 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065322 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003194 same experiment
	EMAGE:21580 same embryo
	EMAGE:21575 same embryo
	EMAGE:21578 same embryo
	EMAGE:21577 same embryo
	EMAGE:21576 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source