EMAGE mouse gene expression spatial database

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EMAGE:21605

Fam114a1 family with sequence similarity 114, member A1 ( MGI:1915553)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002429. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002429_01	euxassay_002429_02	euxassay_002429_03	euxassay_002429_04

euxassay_002429_05	euxassay_002429_06	euxassay_002429_07	euxassay_002429_08	euxassay_002429_09

euxassay_002429_10	euxassay_002429_11	euxassay_002429_12	euxassay_002429_13	euxassay_002429_14

euxassay_002429_15	euxassay_002429_16	euxassay_002429_17	euxassay_002429_18	euxassay_002429_19

euxassay_002429_20	euxassay_002429_21	euxassay_002429_22	euxassay_002429_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21605_wholemount_strong.wlz

21605_wholemount_moderate.wlz

21605_wholemount_weak.wlz

21605_wholemount_possible.wlz

21605_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21605_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

moderate

regional

moderate expression: see section 3 4 5 6 7 8 5 6 7 8 9 0 1

cranium

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3429
Entity Detected:	Fam114a1, family with sequence similarity 114, member A1 ( MGI:1915553)
Sequence:	sense strand is shown >T3429 GCACAACGAAACCTGCCTGCCCTGAAGAAGCATTTTAGTTTAGGGTGAGTGGTGGCAAAATTATAAAGCT GAGAATCATTTCAACAGAAAACTAAAGGGGTTTTAGCCAGGTAGAAACCCCGACCTGCATTTCAAAAATA CCCACTAACCTGATCACACACATGGTAGAGGCAAAGGGTGGAAAGTCGTAAGTTGGCCAAGTGGGGTGCT GGTCCAGGGAGATGCATCAGAGACCTTGCAGGGAGCAGTGGTACTGAACCTGGGCTCTTGTGAGATGCAT CTGTTCTTTCCAGGCTGAGGTTCTCTGGCATGCTTCTGTTTGAGACCATCACTGACATCCCTACCAGTAG TGTCCAAAATAGTCATGCCCAGTGGCTTATCCCAGGGCAAATGATGCCTGCAGTCTTGAAAGGGAGAAAA TCCCTCCTTTGTATTTGCTTTGTTCCTGTGGTAGAAGCCACTGCGTCTGTCCCCTGTGGGCCAAGTAGAT ATCTGAATTCTCTCAGTAGCATTTTTGTGTGACTGTGTTTTGTTTTTT
Notes:	The probe template was PCR amplified from IMAGE:3025903 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3025903 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002429 same experiment
	EMAGE:21600 same embryo
	EMAGE:21601 same embryo
	EMAGE:21602 same embryo
	EMAGE:21603 same embryo
	EMAGE:21604 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source