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EMAGE:21629

Ptpru protein tyrosine phosphatase, receptor type, U ( MGI:1321151)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21629 EMAGE:21629 EMAGE:21629 EMAGE:21629 EMAGE:21629
"Pseudo-wholemount" of euxassay_017923. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017923_01 euxassay_017923_02 euxassay_017923_03 euxassay_017923_04
EMAGE:21629 EMAGE:21629 EMAGE:21629 EMAGE:21629 EMAGE:21629
euxassay_017923_05 euxassay_017923_06 euxassay_017923_07 euxassay_017923_08 euxassay_017923_09
EMAGE:21629 EMAGE:21629 EMAGE:21629 EMAGE:21629 EMAGE:21629
euxassay_017923_10 euxassay_017923_11 euxassay_017923_12 euxassay_017923_13 euxassay_017923_14
EMAGE:21629 EMAGE:21629 EMAGE:21629 EMAGE:21629 EMAGE:21629
euxassay_017923_15 euxassay_017923_16 euxassay_017923_17 euxassay_017923_18 euxassay_017923_19
EMAGE:21629
euxassay_017923_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21629Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21629_wholemount_strong.wlz
21629_wholemount_moderate.wlz
21629_wholemount_weak.wlz
21629_wholemount_possible.wlz
21629_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21629_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50899
Entity Detected:Ptpru, protein tyrosine phosphatase, receptor type, U ( MGI:1321151)
Sequence:sense strand is shown

>T50899
ACTTCTGGCGGCTGGTCTACGACTACGGGTGCACCTCCATCGTCATGCTGAACCAACTTAACCAGTCCAA
CTCCGCCTGGCCCTGCTTGCAGTACTGGCCGGAGCCAGGCCGACAGCAGTATGGGCTCATGGAGGTGGAG
TTTGTGTCTGGCACAGCAAACGAGGATTTGGTGTCCCGAGTGTTCCGGGTGCAGAACTCTTCTCGGCTGC
AGGAGGGTCACCTGCTGGTACGGCACTTCCAGTTTCTGCGTTGGTCTGCTTATCGGGACACGCCTGACTC
CAGGAAGGCCTTTCTGCACCTGTTGGCTGAGGTGGACAAGTGGCAGGCAGAGAGTGGGGATGGGCGCACC
GTGGTGCATTGTCTCAACGGGGGTGGCCGCAGTGGCACCTTCTGCGCCTGTGCCACGGTCTTGGAGATGA
TCCGCTGTCACAGCCTGGTGGATGTTTTCTTTGCTGCCAAAACACTTCGGAACTACAAGCCCAATATGGT
GGAGACCATGGATCAGTATCATTTCTGCTACGACGTGGC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GTTCATCTGTGGTGATGCTG; Reverse Primer - name:unspecified, sequence:TCCAGTGCCACCTCATATAC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_017923 same experiment
 EMAGE:21628 same embryo
 EMAGE:21630 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS