EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21655

B230312A22Rik RIKEN cDNA B230312A22 gene ( MGI:2441854)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005630. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005630_01	euxassay_005630_02	euxassay_005630_03	euxassay_005630_04

euxassay_005630_05	euxassay_005630_06	euxassay_005630_07	euxassay_005630_08	euxassay_005630_09

euxassay_005630_10	euxassay_005630_11	euxassay_005630_12	euxassay_005630_13	euxassay_005630_14

euxassay_005630_15	euxassay_005630_16	euxassay_005630_17	euxassay_005630_18	euxassay_005630_19

euxassay_005630_20	euxassay_005630_21	euxassay_005630_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21655_wholemount_strong.wlz

21655_wholemount_moderate.wlz

21655_wholemount_weak.wlz

21655_wholemount_possible.wlz

21655_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21655_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5546
Entity Detected:	B230312A22Rik, RIKEN cDNA B230312A22 gene ( MGI:2441854)
Sequence:	sense strand is shown >T5546 CCCCCCTGACCCAAGGGGGGCTTCATGCGCCACGTGCAGGCGGAGCTGTCTCCATCCTCAGAGCCAGAGG CTGGCCCTTCACAGCCTCCAGTCAGACAGGGGACCCTCCAGGGTGGCCTGCTCATGGGCTACAGCCCAGC AGGGGGGGCGACATCCCCCGGGGTCTACCAGGTATCCATCTTTTCCCCTTCAGCTGGTGCCTCCGAGCCT CCTAGGGCTCTGAAGCGGCCGGCCCCTCCCACCGAGGGTCCCCGGGAGCTGAAGAGAGGCCCTGGGCTGG GGGCCAGAGAGGGACTACCCCCTGAAGAACCATCTACTGTGGGACTCTTGAGCCCAGAGGGACTGGGTCT GGGACTGGGTGTGGCTAGCCAGCATTTCTCCCACCATGGCCTCTGTGTTGTGGAGCACGGAGGTAATACC ACCTCACCTTGGACTTCAGGGACCCAGAGTACACCCTGGCTCTCATCAAATGCTTCCTTCAATACTTTGC ACACCAGAGACTGGGCTTTCCCAGATCAAGGGGGACAGGGGTGCCTGGGGGAGACTCCAGGGCCAGCCCC TTCAGGCCAGCTGCACACACTTGACACTGATTTGCACAATCTTGCACAAATAGGGGGTAAGAGCCCAGTG GCTAGGGTGGGCAACGGGAGCAACCCCTGGCCTAGGGAGTCCCATGGAACTGCCAATGGGCACAGTCCCG AGCACACACCCCCTGGCCCTGGACCTCCTGGCCCCTGCCCCACCAAGCGAAGGCTGCTGCCTGCTGGAGA AACCCTTGATGTCAGCTCTGAGGATGAGGGGCCACCCCCTCGGAGGCGCCGGGGAACCCTGGGCTGCCCT CTTGCTGCCAATAGTTCTGATGCCAAAGCCACACCCTTCTGGAGCCACCTGCTGCCTGGGCCCAAGGAGC CTGTTTTGGACCCAACAGACTGCAGTCCCATGGGGCGGAGGCTGAAAGGCGCCCGTCGCCTGAAGCTAAG CTCCCTTCGAACTCTCCGGAAGGGGCCAGGCCTGCTGAGCCCTCCCAGTGCCTCCCCCTTTCCCACCCCT GCTGTCAGCCGGACCCTGCTGGGCAACTTTGAGGAATCACTGCTTCGAGGACGCTTTGCACCATCTGGCC ACATTGAGGGCTTTACTGCAGAGATTGGAGCCAGTGGGTCTTACTGTCCCCAACATGTCACGCTGCCCGT CACTGTCACCTTTTTTGATGTTTCTGAGCAAAATGCGCCGGCCCCCTTCCTGGGAGTCGTGGACCTGACT CCTCTGGGGAGGAAGGGTTACAGTGTGCCCAAGGTGGGCACCATCCAAGTGACCTTATTTAACCCCAACC AGACTGTGGTGAAGATGTTCCTTGTGACCTTTGACTTCTCGGACATGCCTGCTGCCCACATGACCTTCCT GCGCCATCGCCTCTTCTTGGTGCCTGTGGGTGAGGAGGGAAATGCTAGCCCCACCCACCGCCTCCTCTGC TACTTGCTGCACCTCAGGTTCCGGAGCTCTCGCTCAGGCCGCTTAAGCCTGCATGGAGATATCCGCCTGC TTTTTTCCCGCCGGAGCCTGGAGCTGGACACGGGGCTCCCCTATGAACTTCAGGCTGTGACTGAGGCCCC CCATAATCCACGTTATTCACCTTTGCCCTGATCGCAAGCATCCTGAACCTAGGTGGGCCCAAAGCCTGCC TGTCCTACCATCCTGGACAGGGTAAACTGTGGAGAAATGGCGAGAGCCCCTTCAGGCTTGAATTAAAGTC CTCATCCCGCTCACGCCCAAATTGACTATTTGGGTGTTTAAAGCTTCGGTCCTTCCATACCCTGTCTGCT CTGGGTACTTCATGTGCCTGTCCCCTCCCTTGGGTTTCCCAGGCCAGCTTAGGTAGTTAGGGAGGAACCA GAGCTACCCTGAGATTGTCCCAAGTTCCCAGGCAAGTAATGCCAGCGTTGCCTCCCTGTCCTGGGCAGGG GCCACTTATTATTTTATTTATTTTTAATTTATAATTTATTCAAATTGGATTGCCTTGGTGACCTCCCAAA CCTGATAATTGGCATCACCCCACTCTCAGATATTGCTCCAACCTCAGGACTTGAAGAAGCTGATGTCGGG GCAAGGGGAGAGCTGCTCTCCTCTGGCTAAGGGCAGAGGGTTTATTCCAGAGGAACTGAGTCACTTAGCC AAAGACTCAGCTTCCCTCGGCTTTCTCTATTCCTTTTCCCTTTCCTGCCCTTCAACCCATCTTCGAAAGC CTGTATGTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACAGCTGTGTGCGTGCATACAAACTTGGG TTTGTGTAGGGTGTGTGTGTGAGAGAGAGACAGAATGTGCATGTATGTGTAACACAGGGGTTTAGCCACC TCTTACCTAGGGCTCAGACTCACTTGTCCCTCTCCTCCTGCCTGTGTCTCCTTGCTTTGGTGTCCTGACT GAGGAAGGTGTCCAGGGACAGAGTCAGGCCTAAGGACTGGGGAACCTCCTCTCACCTGACCAGACTCTGA CCCACCTACCTCAGCTGGGATGAGGGGTGCCCCTCATTCAGTCACATGGTTCCCAGTTACTCCACTCCTC ACTTAAGACTCTGACCCAGCCTCAACCCTTAACTCTTGGGGCTTGGCTGAGGGGAACAAGCATTTGCTGA AACTTGAAAAACAAAACAAACAAACAAAAAAATGGGAAAAATTGTACCTGGTACATTTTTTAAGGAAAAA AAAAAGAATAAATTCTTGTTTGGAAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6467750 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6467750 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005630 same experiment
	EMAGE:21654 same embryo
	EMAGE:21656 same embryo
	EMAGE:21653 same embryo
	EMAGE:21657 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source