EMAGE mouse gene expression spatial database

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EMAGE:21666

Plagl1 pleiomorphic adenoma gene-like 1 ( MGI:1100874)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009026. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009026_01	euxassay_009026_02	euxassay_009026_03	euxassay_009026_04

euxassay_009026_05	euxassay_009026_06	euxassay_009026_07	euxassay_009026_08	euxassay_009026_09

euxassay_009026_10	euxassay_009026_11	euxassay_009026_12	euxassay_009026_13	euxassay_009026_14

euxassay_009026_15	euxassay_009026_16	euxassay_009026_17	euxassay_009026_18	euxassay_009026_19

euxassay_009026_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21666_wholemount_strong.wlz

21666_wholemount_moderate.wlz

21666_wholemount_weak.wlz

21666_wholemount_possible.wlz

21666_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21666_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

hypothalamus ventricular layer

weak

regional

weak expression: see section 8 9 0 1 2 3 4 5

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 0 1 2 3 4

telencephalon ventricular layer

weak

regional

weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

pons ventricular layer

weak

regional

weak expression: see section 5 6 7 4 5 6

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6

midbrain ventricular layer

weak

regional

weak expression: see section 4 5 6 7 8 9 0 1 2 3 4

otic capsule

moderate

regional

moderate expression: see section 5 6 7 4

neural retina

moderate

regional

moderate expression: see section 2 3 4 5

exoccipital bone

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0

temporal bone petrous part

strong

regional

strong expression: see section 1 2 3 4 6 7 8 9 0

orbito-sphenoid

strong

regional

strong expression: see section 1 2 3 7 8 3 8 9 0

viscerocranium

strong

regional

strong expression: see section 9 0 1 2 3 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35516
Entity Detected:	Plagl1, pleiomorphic adenoma gene-like 1 ( MGI:1100874)
Sequence:	sense strand is shown >T35516 GCTGATGCAAGAGAATATGCAGGCAGGAGATTACCAGAGCAATTTCCAACTCATTGCGCCTTCAACTTCG TTCCAGATAAAGGTTGATCCCATGCCTCCTTTCCAGCTAGGAGCGGCTCCCGAGAACGGGCTTGATGGTG GCTTGCCACCCGAGGTTCATGGTCTAGTGCTTGCTGCCCCAGAAGAAGCTCCCCAACCCATGCCGCCCTT GGAGCCTTTGGAGCCTTTGGAGCCTTTGGAGCCTTTGGAGCCGATGCAGTCTTTGGAGCCTTTGCAGCCT TTGGAGCCGATGCAGCCTTTGGAGCCAATGCAGCCTTTGGAGCCGATGCAGCCTTTAGAGCCTTTGGAGC CTCTGGAGCCGATGCAGCCTTTGGAGCCGATGCAGCCTTTGGAGCCTATGCAGCCAATGCTGCCAATGCA GCCAATGCAGCCAATGCAGCCAATGCAGCCAATGCTGCCAATGCAGCCAATGCTGCCAATGCAGCCAATG CAGCCAATGCAGCCAATGCTGCCAATGCCAGAGCCGTCTTTCACTCTGCACCCTGGCGTAGTTCCCACCT CTCCTCCCCCAATTATTCTTCAGGAGCATAAGTATAATCCTGTTCCTACCTCATATGCCCCATTTGTAGG CATGCCCGTCAAAGCAGATGGCAAGGCCTTTTGCAACGTGGGTTTCTTTGAGGAATTTCCTCTGCAAGAG CCTCAGGCGCCTCTCAAGTTCAACCCATGTTTTGAGATGCCTATGGAGGGGTTTGGGAAAGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98829. Forward Primer - name:098829_F_cDNA_Plagl1, sequence:GCTGATGCAAGAGAATATGCAG; Reverse Primer - name:098829_N_SP6_cDNA_Plagl1, sequence:ACTTTCCCAAACCCCTCCATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009026 same experiment
	EMAGE:21667 same embryo
	EMAGE:21669 same embryo
	EMAGE:21668 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source