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EMAGE:21682

Pogz pogo transposable element with ZNF domain ( MGI:2442117)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682
"Pseudo-wholemount" of euxassay_005129. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005129_01 euxassay_005129_02 euxassay_005129_03 euxassay_005129_04
EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682
euxassay_005129_05 euxassay_005129_06 euxassay_005129_07 euxassay_005129_08 euxassay_005129_09
EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682
euxassay_005129_10 euxassay_005129_11 euxassay_005129_12 euxassay_005129_13 euxassay_005129_14
EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682
euxassay_005129_15 euxassay_005129_16 euxassay_005129_17 euxassay_005129_18 euxassay_005129_19
EMAGE:21682 EMAGE:21682 EMAGE:21682 EMAGE:21682
euxassay_005129_20 euxassay_005129_21 euxassay_005129_22 euxassay_005129_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21682Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21682_wholemount_strong.wlz
21682_wholemount_moderate.wlz
21682_wholemount_weak.wlz
21682_wholemount_possible.wlz
21682_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21682_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6315
Entity Detected:Pogz, pogo transposable element with ZNF domain ( MGI:2442117)
Sequence:sense strand is shown

>T6315
AATACTCAAGCTATGCATCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGA
ATTCGCCCTTAAAGTTGTGCTTTAAAACGTGCATGAACCACCTTGAAACATCTAGAATGGATAGATTTTT
CTGTCCTCACCACCCTCCACCAAAATATAACAGAAACAATATTTTTTTGCACTTGTAACCCTTGACCCCT
TTCCTGATTTTTCACATCATCCTTCACTCTGGACAAAGGAGCACATGTGTCCCTGGCAAGCAGAGGTACT
GAGTGAGGTGATGAGTGTTTCAGGTGGAAGCCATTCCTAGTCTCTGTCTTCAACAAGCCCGATGGCGTAG
TCCCTTTCCTCTAGAACTTTGTCCTGTTAAGCTCGCTGACGGCACACCTGCTCATCACAGGTTTAACTGG
CATGTTAAGTGTTGAACTAGAAAGACAGACTGCTTCAACTCCTGCTTACACGTGGCTTTCCTGTTCCTCT
CATATTCAGAGAAACGGATGTGAAAAGCATAGAGGTGTGCACAGAGAAAGACATGAATCTTTATTTTTCA
CTGCCAACTTCAAGGAAAAATTTTTCTACAGTTTGCATGAGGGATTTTTT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AAAGTTGTGCTTTAAAACGTG; Reverse Primer - name:unspecified, sequence:CTTTGAAGTGTAGTCCGTCAC. The reverse primer contains a 5' extension containing an T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using T7 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005129 same experiment
 EMAGE:21685 same embryo
 EMAGE:21683 same embryo
 EMAGE:21684 same embryo
 EMAGE:21681 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS