EMAGE mouse gene expression spatial database

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EMAGE:21746

Slc29a1 solute carrier family 29 (nucleoside transporters), member 1 ( MGI:1927073)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019687. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019687_01	euxassay_019687_02	euxassay_019687_03	euxassay_019687_04

euxassay_019687_05	euxassay_019687_06	euxassay_019687_07	euxassay_019687_08	euxassay_019687_09

euxassay_019687_10	euxassay_019687_11	euxassay_019687_12	euxassay_019687_13	euxassay_019687_14

euxassay_019687_15	euxassay_019687_16	euxassay_019687_17	euxassay_019687_18	euxassay_019687_19

euxassay_019687_20	euxassay_019687_21	euxassay_019687_22	euxassay_019687_23	euxassay_019687_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21746_wholemount_strong.wlz

21746_wholemount_moderate.wlz

21746_wholemount_weak.wlz

21746_wholemount_possible.wlz

21746_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21746_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 phalanx

weak

regional

weak expression: see section 7

hindlimb digit 2 phalanx

weak

regional

weak expression: see section 7 4

hindlimb digit 3 phalanx

weak

regional

weak expression: see section 7 8 4

hindlimb digit 4 phalanx

weak

regional

weak expression: see section 4

hindlimb digit 5 phalanx

weak

regional

weak expression: see section 4

vertebral axis musculature

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

thymus primordium

strong

regional

strong expression: see section 1 2 3 4 5 6 7

tongue

weak

regional

weak expression: see section 3 4 5 6 7 8 9

liver

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9

liver lobe

strong

regional

strong expression: see section 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

testis

weak

regional

weak expression: see section 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56008
Entity Detected:	Slc29a1, solute carrier family 29 (nucleoside transporters), member 1 ( MGI:1927073)
Sequence:	sense strand is shown >T56008 AACTCTCCACCCACCAACAGAAACCAGTCTATCAAAGCCATACTTAAGAGTATCTGTGTCCCGGCTCTGT CTGTCTGCTTCATCTTCACGGTTACCATTGGGTTGTTCCCTGCTGTGACTGCTGAGGTGGAATCCAGCAT CGCAGGCACAAGTCCCTGGAAAAGCTACTTCATTCCCGTGGCCTGTTTCTTGAATTTCAATGTCTTTGAC TGGCTAGGCCGGAGCCTCACTGCTGTCTGCATGTGGCCTGGCCAGGATAGCCGCTGGCTGCCGGTTTTGG TCGCCTCGAGGATTGTGTTTATTCCCCTGTTGATGCTCTGCAACGTGAAGGCTCGCCACTGCGGCGCGCA GCGGCACCACTTCGTCTTTAAGCATGACGCCTGGTTCATCGCCTTCATGGCTGCCTTTGCCTTCTCCAAT GGCTACCTCGCCAGCCTCTGCATGTGCTTCGGGCCCAAGAAAGTCAAACCAGCTGAGGCGGAGACAGCAG GAAACATCATGTCCTTCTTTCTGTGTCTGGGCCTGGCTCTGGGAGCTGTGTTGTCCTTCTTGTTAAGGGC ACTTGTGTGACCCTGTGGGGACAGAAGAACTACACTGCCTGCTTCCTGCTCACTTCCTTCCCTGCCAGGG ACGAGCAGGGGTCGAGAGGGGCTGTTCTTCTAGCTGACTTCTGCTTTCCTCTGGACTGTGCTTCGCCCAG CTGTCCAGGAGCCAGCGATGGCCTGCGGGTGGACTTGGGATTCAGGGTCAGAATGGCAAGGGCTCAATGG CCTCTGACTGACAGCTCCGACTGATGCCCGCTTACTCCAAGCACAAGAGACTCCAGGGCCAAGAGAGATC TGTCCGCCTGCCTATCACAGGATAGGGCGGAGGCGGATGGCTGATTGGTGTCGTGTGACC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AACTCTCCACCCACCAACAG; Reverse Primer - name:unspecified, sequence:GGTCACACGACACCAATCAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_019687 same experiment
	EMAGE:21745 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source