EMAGE mouse gene expression spatial database

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EMAGE:29251

Mtrr 5-methyltetrahydrofolate-homocysteine methyltransferase reductase ( MGI:1891037)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005598_11	euxassay_005598_12	euxassay_005598_13	euxassay_005598_14	euxassay_005598_15

euxassay_005598_16	euxassay_005598_17	euxassay_005598_18	euxassay_005598_19	euxassay_005598_20

euxassay_005598_21	euxassay_005598_22	euxassay_005598_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4947
Entity Detected:	Mtrr, 5-methyltetrahydrofolate-homocysteine methyltransferase reductase ( MGI:1891037)
Sequence:	sense strand is shown >T4947 CCCACGCGTCCGGTCTCACTGTTAGAGTTGCAGCTGATGACCTGAGGTGATGAGAAGGTTTTTGTTACTT TATGCTACACAGCGAGGACAGGCAAAGGCCATAGCAGAAGAAATAAGTGAGCAGGCTGTGTCACACGGGT TTTCCGCAGATCTTCACTGTATCAGTGAGTCGGAAAAGTATGATCTGAAAACAGAAACTGGCCCTCTGGT GATGGTTGTATCTACCACGGGTACAGGAGACCCACCTGACACAGCCCGGAAGTTTGTTAAAGAAATACAC AACAAGACACTCCCCACGGACTATTTTGCTCACCTGCGGTATGGATTATTGGGCTTGGGTGACTCAGAAT ACACATACTTCTGCAATGGAGGGAAAGTAATTGATAAACGACTTCAGGAGCTTGGAGCCCAGCGTTTCTA CGACACCGGACATGCCGATGACTGTGTCGGTTTAGAGCTTGTGGTAGAGCCGTGGATTGATGGACTCTGG GCTGCTCTCACAAAGCACTTTAAGTCACTTGGAGGACAAGAAAACATGAGTGACACACTCTCGCGAGCAT CAGATGCCCCCTTGAGCACTGCCATGAAGCCGGAGTTGTTGCACATCCAGTCACAATTTGAACTGCTGAG ATTAGAGGACGTGGGGGAGAGGGATTCTGAGCTTCGGGAACAAAATGAGACAAACAGGGGTCAGCAGGGT CGGATCGAAGACTTTGACTCCTCGCTTGTGCACTCGGTCCCCCCACTCTCCCAGAGCTCTCTAAGTATTC CTGCTGTGCCCCCAGAGTATTTGGAGGTCCATCTTCAAGAGTCTCTTGGCCAGGAGGAAAACCAAGCATC TGTGCCTTCAGGGGATCCAAGTTTTCAAGTTCCAATTTCAAAAGCCATTCGTCTGACTACAAATGATGCC GTAAAGAGCACTCTGCTGTTGGAACTGGACATTTCAAAAATAGAGTTTTCCCATCAACCTGGAGACTCCT TCAATGTGACCTGTCCAAACAGTGATCGTGAGGTAGAAGAACTGCTCCAAAGGCTGCAGCTGGCTGATAA ACGAGCGCACCGTGTCATCTTGAAAATTAAGACGGACACTAAGAAGAAAGGAGCTGCCCTGCCTGCGCAT GTGCCTGAGGGGCGTTCTCTCCAGTTCATCCTCACCTGGTGTCTTGAAATACGAGCAGTTCCTAAAAAGG CCTTTTTGCGGGCCCTTGCGGAGCACACCAGCAGTGCCACTGAAAAGCGGAGGCTGCAGGAGCTGTGCAG TAAACAAGGAGCCGCTGATTACAACCGCTTTATCCGAGATGCCAGCGTCTGCCTGCTGGACCTCCTGCTC ACCTTCCCGTCCTGCCAGCCTCCGCTCAGCCTCCTGCTGGAGCACCTTCCTAAACTTCAGCCTCGACCGT ACTCATGTGCAAGCTCCAGCTTACGCCACCCAGACAAGCTTCACTTCGTGTTTAACATCGTGGAGTTCCC ACCGAGCACCACTGCAGCCTCCCCGCGGAAGGGAGTGTGCACGGGCTGGCTAGCCACATTGGTTGCTCCA TTCCTTCAGCCAAACACAGACGTTTCGAATGCAGACAGTGGGGACACTCTGGCTCCTGAGATACGTATCT CTCCTCGGGCAACAAATGCTTTCCACTTACCAGAGGACCCGTCGGCCCCCATCATAATGGTGGGTCCAGG AACTGGCGTAGCCCCCTTTGTTGGCTTCCTCCAGCACAGAGAAAAACTTCAAGAACAACACCCAGATGGG AAATTTGGAGCAATGTGGCTGTTCTTTGGCTGCAGACACAAGGACAGAGACTACTTGTTCAGGGAGGAGC TCAGGCATTTCCTCAAGACTGGGGTCTTGACTCATCTGAAGGTCTCGTTTTCAAGAGACGCTGCCCCTGA CGGGGAGGAGGCCCCAGCAAAGTATGTGCAAGACAACCTCCAGCGGCACAGCCAGCAGGTGGCCAGGACC CTCCTTCAGGAGAATGGCTACATTTACGTGTGTGGAGACGCCAAGAATATGGCCAAGGACGTGAACGACA CCCTCATAGGAATCATAAGCAATGAAGCTGGTGTTGACAAACTAGAAGCAATGAAGACGCTGGCAACGTT AAAGCAAGAGAAACGATATCTGCAGGACATCTGGTCCTAAACTGGAACAGAGACGCTTGGAATTTGTTTT TGTTTTTGTTTGTTTGTTTGTTTTTTCTTGAAGTGCCAAAGGTCAATTTTACAGAAGATGAGTTTAAAAA TGTTTGGACATTTCTTGGAGGTGGCTGTGTGGGGAACGGCTTGCTTACAGGTACAGCAGGTACAACCGCC TGACAGTCTCACAGGTTGTATCCCACTCCTTACCTGTGGCCCCACCCAGCACATCACTACGTGGAGGGGG AAGAGGCAGCTCCCACCTGGCACCCATTGCACATAGGCTCCCCAGGAGGACAGATCATCCACGGCTCACC TGTGCCTGGGCACATCTTGGAGAGCTTGTGGACATGCTATCTTTCAATGAAGTATATTCAAAGGATATTT ATATTGGTGATATTTAAATTAACACAGACATGCAAAGCATTCTTTTGCATGTCTTTATTTAATTGTGGTT TTATTATCAAAATTATATTTCTGATAAAATATTTTAATTTTGAGGTAATCCTTACAAAATGAAGGTTTTC ATGCAACTAGAAATATATTTTGAAATATATTTGATATAGTACTCTGCCATTTTCATCATGTTAACTTTTA GTGTCACCATCTTACAGCTTAGGGGCATTTGCATTCCCCTGACAAAGGTATCATGTGTACAGCTCAGTAG TGTTCTGCCCTGTCCTTCTCATGGACTCCATTCATGTCTGCTCCTCACTAACAGACTTGGGTTTTCCCAA TACCTGACTATACTTTTGTGATTCAGTTCAGCTTAATGAACTCCAGTTACGCCGGGCGTGGTGGCGCACG TCTTTAATCCCAGCACTTGGGAGGCAGGCAGAGATAGGCGGATTTCTGAGTTTGAGGCCAGCCTGGTCTA CAGAGTGAGTTCGAGGACAACCAGGGCTACACAGAGAAACCCTGTCTCGAAAACCAAAAACAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5132457 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5132457 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005598 same experiment
	EMAGE:30923 same embryo
	EMAGE:29256 same embryo
	EMAGE:29796 same embryo
	EMAGE:31963 same embryo
	EMAGE:31962 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source