EMAGE mouse gene expression spatial database

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EMAGE:29256

Cflar CASP8 and FADD-like apoptosis regulator ( MGI:1336166)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005593_01	euxassay_005593_02	euxassay_005593_03	euxassay_005593_04	euxassay_005593_05

euxassay_005593_06	euxassay_005593_07	euxassay_005593_08	euxassay_005593_09	euxassay_005593_10

euxassay_005593_11	euxassay_005593_12	euxassay_005593_13	euxassay_005593_14	euxassay_005593_15

euxassay_005593_16	euxassay_005593_17	euxassay_005593_18	euxassay_005593_19	euxassay_005593_20

euxassay_005593_21	euxassay_005593_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4121
Entity Detected:	Cflar, CASP8 and FADD-like apoptosis regulator ( MGI:1336166)
Sequence:	sense strand is shown >T4121 CCTCGATTCAGTTTTGGTGAAATGGCGGCCATTCTCATCTTCTCGGAGCCCTTCTCTTTGCTCACACTCG CGATTCTGCCCCCGCAAGAGTACTCTGGGGACGTCCTTATCGCTGGGAGAGAGCGGTCGCATCTCATTCC TGAACCTGTTGGGCTCGCTGGGCGCGTCACCTGGCTAGCAGCTTGGGCCAGCTCCGCAGGCTAACTTTCC TGGACGCCTACTGAAGAGTCTGCTCCTCTCTGTGTTCTTTCCAGTGTCTCGTTCGATCCAGTTTTCTGGT GGTCTCCAGCGAAGACAGGCGACAAAGCCGTTGTTGAGTGGGATGGGCCGGCGACCGCCCGGTAGTGTCT CTATTGCAAGAACTCTGAGAGAAATGAAGAGAGTCCTCAGCAATGATGTTGGCTTCTCGTGGTTCCCAGA GCCCTGCTTAATGGATGGAGACTGGACGAGAACCTGGCTGCTGTGGTTCTGAACATGGCCCAGAGCCCTG TGTCTGCCGAGGTCATTCACCAGGTGGAAGAGTGTCTTGATGAAGACGAGAAGGAGATGATGCTCTTCCT GTGTAGAGATGTGACTGAGAACCTGGCTGCACCTAACGTCAGGGACCTCCTGGATAGCTTAAGTGAGAGA GGCCAGCTCTCTTTTGCTACCTTGGCTGAATTGCTCTACAGAGTGAGGCGGTTTGACCTTCTCAAGAGGA TCTTGAAGACAGACAAAGCAACCGTGGAGGACCACCTGCGCAGAAACCCTCACCTGGTTTCTGATTATAG GGTCCTGCTGATGGAGATTGGTGAGAGCTTAGATCAGAACGATGTATCCTCCTTAGTTTTCCTTACAAGG GATTACACAGGCAGAGGCAAGATAGCCAAGGACAAGAGTTTCTTGGATCTGGTGATTGAATTGGAGAAAC TGAATCTAATTGCTTCAGACCAATTGAATTTGTTAGAAAAATGCCTGAAGAACATCCACAGAATAGACTT GAACACAAAGATCCAGAAGTACACCCAGTCCAGCCAAGGAGCAAGATCAAATATGAATACTCTCCAGGCT TCGCTCCCAAAATTGAGTATCAAGTATAACTCAAGGCTCCAGAATGGGCGAAGTAAAGAGCCAAGATTTG TGGAATACCGTGACAGTCAAAGAACACTGGTGAAGACATCCATCCAGGAATCAGGAGCTTTTTTACCTCC GCACATCCGTGAAGAGACTTACAGGATGCAGAGCAAGCCCCTAGGAATCTGCTTGATCATTGATTGTATT GGCAACGACACAAAATATCTTCAAGAGACCTTCACTTCCCTGGGCTATCATATCCAGCTTTTCTTGTTTC CCAAGTCACATGACATAACCCAGATTGTTCGCCGATATGCAAGTATGGCCCAACATCAAGACTATGACAG CTTTGCATGTGTTCTGGTGAGCCTAGGAGGCTCCCAAAGCATGATGGGCAGAGATCAAGTTCACTCAGGG TTCTCCTTGGATCATGTCAAGAACATGTTCACGGGGGACACGTGCCCTTCTCTCAGAGGGAAGCCAAAGC TCTTTTTTATTCAGAACTATGAGTCGTTAGGTAGCCAGTTGGAAGATAGCAGCCTGGAGGTAGATGGGCC ATCAATAAAAAATGTGGACTCTAAGCCCCTGCAACCCAGACACTGCACAACTCACCCAGAAGCTGATATC TTTTGGAGCCTGTGCACAGCAGACGTATCTCACTTGGAGAAGCCCTCCAGCTCATCCTCTGTGTATCTGC AGAAGCTCTCCCAGCAGCTGAAGCAAGGCAGGAGACGCCCACTCGTGGACCTCCACGTTGAACTCATGGA CAAAGTGTATGCGTGGAACAGTGGTGTTTCGTCTAAGGAGAAATACAGCCTCAGCCTGCAGCACACTCTG AGGAAGAAACTCATCCTGGCTCCTACGTGAGAACCCCAGACCGTTGGTGTTCTTGGTATATCATCCAGGG TGGCGGCTTGGAGCAGAGCTTGGCGGTTACGGCTGCTTCTGGCTGCTTCTGGCTCTGCCGTGAGTCCTGG CCTAGGGTTCTCCTGTGCACAGGCATGAGCCGTAACCCTGTGCCTGGGAAACGTCTCACTCCCGCCGCCG TGCCTTTACCTCTCTAAACTTCCCTACTTACATTCCTTAGTCGGATGTTTTGCCAGAGTGTGGAGAACAG TAAGACATAAACCTATTGTTTGTTTGTTTTTTTGGGGGGGAGGTTATCTACCAAGTTATACCAAGTTATT GTATGGGTGTATAGTGTATAGTGGTTCAAGATTTGACACTGAATGTAACTTGAGACTTACCTGAGTTTGT CATGCGACTGGGTAAATTGTTTCTATGGCACATCTAATCATTTAATAAGTAATTACCTCATTAAGTACCC ATTGCTTCAGGACTTTCACATTGGCCACCAATTTCTGTGACCCAGCTCCACATTTATATTCTCTTTCTGC AAAACCAAATTTCATTATGTCTGTTTAATATCTACAGTCTAATGCTTTGTAAGACATCTAGATAGAAAAA TAGTTACCCATGAGCACAGGAGGGCTGGCCTGACCCTCACCAGCTGTGCAGTGGCTTCGGTGAAGGAGAA TGAGCCCTACTCCTTGAAGGTTGTAGTGCTTGGGAGAGCAGTCTGTACCTTGCCTGGGCAGCACAGTAGA GCCAGCCCCAAGAACACAACAGTGAGTGGGGGAGCTTGCCCTGGTTGGCTCAGGATCAGGAACAGGAGGG ATGACCAACTTGGGGCTTTGAGGTGGCCCACCCCAGCATCCATATCATCTGTGAACTGCCAGAGCCTGTG AAGGGGCGGGTCCTGTAGAACTAAGGCTGCAGGATCTCCATGACACAGGGCAACAACAGGGTATCTGAGA AGGGTCCCCGTGAGGGTCCAGTATTTATAGTGCACCAGAAGCCAGAGGCCTCGGATCAGACAATGACCCA TTGCACTGAGTAAAGATGTAAGTGAATGAGTGAAGATGTGTGGGCACACGGAAATACTGAGGGACACACA CAAGCTTTTATGGAGATGTTTGTTTGTTTGTTTGTTTGTTTTTTGTTTCTTTGGCAGGAACAGATTGCAA GGGCAGAGAGTAGATAAGGAAGCTGGAGACATGAGTGGGGTTGGGTGCATGATATAGAATTCACAAAGAA TCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4460554 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4460554 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005593 same experiment
	EMAGE:30923 same embryo
	EMAGE:29251 same embryo
	EMAGE:29796 same embryo
	EMAGE:31963 same embryo
	EMAGE:31962 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source