EMAGE mouse gene expression spatial database

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EMAGE:29578

Coro2b ( MGI:2444283)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006159_11	euxassay_006159_12	euxassay_006159_13	euxassay_006159_14	euxassay_006159_15

euxassay_006159_16	euxassay_006159_17	euxassay_006159_18	euxassay_006159_19	euxassay_006159_20

euxassay_006159_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8895
Entity Detected:	Coro2b, ( MGI:2444283)
Sequence:	sense strand is shown >T8895 GATGCTCGGCCGCGGCTGACATCAGCCAGCGGCCTCCCGAGCGCGCGGTCCCCAGCCCGGCCCAGCCGCC TGCCGCCGCCCGCGCCCAGCCCCACCGCCCGCTTTCTGTATGACTGTCACAAAGATGTCCTGGCGACCGC AGTACCGCAGTTCCAAGTTTCGGAACGTGTATGGGAAAGCGGCCAACAGGGAGCACTGCTTTGACGGGAT CCCCATCACCAAGAATGTACATGACAACCACTTCTGTGCAGTCAACGCCCGCTTCCTGGCCATAGTCACT GAGAGCGCAGGAGGTGGCTCCTTCCTCGTCATCCCCCTGGAGCAGACAGGCAGAATCGAGCCAAACTACC CCAAGGTATGCGGTCATCAGGGCAACGTGCTGGACATCAAATGGAACCCCTTCATTGACAACATCATCGC CTCTTGCTCCGAGGACACTTCGGTGCGGATCTGGGAGATCCCTGACGGTGGGCTGAAGCGGAACATGACC GAGGCGCTCCTGGAGCTCCACGGACACAGCCGCCGTGTGGGGCTGGTTGAGTGGCACCCCACGACCAACA ACATTCTCTTCAGCGCTGGCTATGACTACAAGGTCCTCATCTGGAACCTGGACATAGGCGAGCCAGTGAA AATGATTGACTGCCACACCGACGTGATCCTCTGCATGTCCTTCAATACAGACGGCAGCCTGCTCACCACC ACATGCAAGGACAAGAATCTGCGTGTGATTGAACCCCGATCCGGCCGTGTTCTACAGGAGGCAAACTGCA AAAACCACAGAGTGAATCGGGTAGTGTTCCTGGGCAACATGAAGCGGCTCCTCACGACAGGGGTCTCCAG GTGGAACACAAGACAGATCGCCCTCTGGGACCAGGAGGATCTGTCCATGCCAATGATCGAAGAAGAAATT GATGGACTCTCAGGCCTCCTGTTCCCATTCTATGATGCTGATACCCACATGCTCTACCTGGCTGGAAAGG GGGATGGAAACATCCGGTACTATGAGATCAGCACGGAGAAGCCCTACCTGAGCTACCTCATGGAGTTCCG CTCCCCAGCCCCACAGAAAGGCCTAGGTGTCATGCCCAAGCATGGGTTGGATGTGTCTGCCTGCGAGGTC TTCCGCTTCTACAAGCTGGTGACCCTCAAGGGCCTGATTGAACCCATCTCCATGATTGTACCCCGGAGGT CAGATTCCTACCAGGAGGACATTTATCCCATGACACCAGGCACAGAGCCAGCGCTGACCCCTGATGAATG GCTGGGAGGCATCAACCGAGATCCCGTGCTGATGTCTCTGAAGGAAGGCTATAAGAAGTCTTCAAAAGTG GTATTTAAGGCCCCCATCAGAGAAAAGAAGAGCGTTGTCGTCAACGGCATAGATTTATTAGAAAATGTCC CACCCAGGACAGAGAATGAGCTCCTCAGGATGTTCTTTAGGCAGCAGGATGAGATTCGAAGGCTGAAGGA GGAGCTGGCACAGAAGGACATCCGGCTCCGTCAGCTCCAGCTCGAACTGAAAAACCTGCGCAACAACCCC AAGAACTGCTAGCCCCGCCTCGGGCTGTGTTAGCCCCGCCTTGGGCTGAGTAAGCCCCGCCTCGGGCTGT TTTCCAAGCCGATCTCTCCGTTGTTTTTACTGGTCCCTAAACTGCCTCTTATCTGTAGAGCCCAGAGACA GGGCCTGGACTGGAGCTGGGGACCAGTTGGTAGACCCTACCTACCACCTCAAGAACTGGAAGCTGACCTT TGACCTCCTGACTTCATGCTAACATCGGTCCCCCAGCCTCTCCTGGAAAGCCCACCCCTGACTGGCTGCG GCTTCTCCTTGCAGCCCCAAGAGCCAGGCTTTCCCCCTTGGCTCCCGGCAAGTCTCCTCAGAGGGACAGG ATGAGTGGGATTACAGCCTGGTTTAGATCTTGAATGTGTCCCCTGCAGAGGGGCTGCCAGGACCTATCTA CACAGGAACCAGTCGCTCTGACCCCAGCATTCCTTCTGAAACACAGAGAGGGCTGGATTGTGGGGAGAGC GCTCTGCCCACCTTCTGCCTCCTTCTGCTCCTGCTGCCGAGCAAGCTCAAGTCATCTCACCCAATGGTTG AGCGCCTGGCAGCCCGCCCTTTTCTTTCTGAACTCCCTCCCACCCCTTTGTCTTCAGGGAATGAAGAGGA TGTGCTCCATGGCCATAATGACCCTTTGCCTACCTACCCTGTGGTTCTCTTCAAAGCTCTTTTACACCAC TTTTCCATTTAATTCGTGAAGCAGGCAGAGTAAGGATTAGTGTCCCTACCTGTCAAATGACAGAACCGAG GGGTCACTCAGAGGGTCAGCAGCAGACCAAGTACCCTTGGTATCCAGAGCTTTCGGCCAGGGCCCTTCCC ACTGTATTGAGATGCCAATCCCCTTGTGGGCTTCCCCAGAAAGAGACCTGGAAGCCACTTCCACTGTCTG CCCAACACCAGTAGTGCCAACACGCAGGCTGAGGCTCTCACACTCAGTGCCCTGGACCCTCCTGGCCTGC GCTCCTTGCTCTCACTCTCTGCTGGGGGGGGGGGGGGGGCTCCATCTGACAGTGAGGCCTCTTCCTGGAA GCTGAGGCAGAGAGAAAGAATAGAGTTTGGCCCTTGGGCAGGCAGAGCAAGATGGCTTCCAGGGACGCTC TGCCTGTGTCTCCCTCCACCCCGCTCCCTTCCCACTCCCAACCCTGCTATCTCAGCCACTTTCAGCCTTA CACACACAGGATGACCACAGCCACTCCACCCACATAGCACTTTAAAGTTTACCCAGGTTTTTGACACATA GATCTCACCGACTCGTGCATCTGCCCTACTTTACAGTGAAGGTAACCAGGAAATGGCCCTCTGCACGCAG CTCTGGGATGCGGCATCTCTTTGGTCCTCTACACGCTTGCTTTAGCCCCTCTCCCCTCCCTCTCTCCATT CTCCACCTAACACATGGAGCTCAAAAGCACTGGTGTCAGGACAGTCACCGCCCTTTGGGTAGAGGAGATG TGGGGACCTCGGTCACCGGCTGGGATCTGCTATACATAGGACACTTCTTGCCTGCCCACCCTGCAAAACT TCCCAACTGCAGATGCCCTCCAGGCGAGCTGAGGTGAGATTTCCCCAGCCCTGGGGCCTCTCCTCTCCAA CCACCGACGTTCCCCACTCCCAGCGTGCTAAAGCGAGTTCTCCTCCCCACCACCACAGCTAGACTGTCAG CTCTCATCTCCCCTCCACACTTCTGGAATTTTCTTTCTCTCCATTGACCTTTGTGGTCTTCTGTGATTAT TTATGCTGCCTCCCAAGGTTAGAATTGAAATAAAATGTTTTCAGGTTATCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416110 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416110 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006159 same experiment
	EMAGE:29747 same embryo
	EMAGE:29581 same embryo
	EMAGE:31540 same embryo
	EMAGE:29752 same embryo
	EMAGE:29746 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source