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EMAGE:29726

Prps1 phosphoribosyl pyrophosphate synthetase 1 ( MGI:97775)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T471
Entity Detected:Prps1, phosphoribosyl pyrophosphate synthetase 1 ( MGI:97775)
Sequence:sense strand is shown

>T471
CCTCGAGNCTGTTGGCCTACTGGAAGATGGCGGAGTAGCAACGCGGTACTGTTGGTGTTCAGACTGCCTG
CTGACTTCCGTTCCCGTGTTGGCAGCGGCGGCGGCGGCGACCACTGAGCACGTTGAGGTAGTTACCCAAG
ATGCCGAATATCAAAATCTTCAGCGGGAGCTCCCACCAGGACTTATCCCAGAAAATCGCTGACCGCCTGG
GCCTTGAGCTAGGCAAGGTGGTGACTAAGAAATTCAGCAACCAGGAGACCTGTGTGGAAATTGGCGAAAG
CGTTCGTGGAGAAGATGTCTACATTGTTCAAAGTGGGTGTGGTGAGATAAATGACAGTCTAATGGAGCTT
TTGATCATGATTAATGCTTGTAAGATCGCTTCAGCCAGCCGGGTTACTGCAGTCATCCCATGCTTCCCTT
ACGCCCGCCAAGATAAGAAGGATAAGAGCCGGGCTCCAATCTCAGCTAAGCTTGTTGCAAATATGCTATC
TGTAGCAGGTGCAGATCATATTATCACCATGGATCTACATG
Notes:The probe template was PCR amplified from IMAGE:1451528 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1451528 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006197 same experiment
 EMAGE:31779 same embryo
 EMAGE:29976 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS