EMAGE mouse gene expression spatial database

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EMAGE:29746

Mars ( MGI:1345633)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006165_01	euxassay_006165_02	euxassay_006165_03	euxassay_006165_04	euxassay_006165_05

euxassay_006165_06	euxassay_006165_07	euxassay_006165_08	euxassay_006165_09	euxassay_006165_10

euxassay_006165_11	euxassay_006165_12	euxassay_006165_13	euxassay_006165_14	euxassay_006165_15

euxassay_006165_16	euxassay_006165_17	euxassay_006165_18	euxassay_006165_19	euxassay_006165_20

euxassay_006165_21	euxassay_006165_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8894
Entity Detected:	Mars, ( MGI:1345633)
Sequence:	sense strand is shown >T8894 CCGAGGCACGATGAGACTGTTCGTGAGCGAGGGTTCCCCGGGGAGCCTGCCCGTGCTGGCTGCGGCCGCG AGGGCCCGGGGTCGGGCGGAGCTGCTCATCAGCACCGTAGGCCCCGAAGAGTGTGTGGTACCATTCCTTA CCCGGCCTAAGGTCCCTGTCTTGCAGCTGGATAGTGGCAACTACCTCTTCTCTGCTAGTGCAATCTGCCG ATATTTTTTTCTGTTATGTGGCTGGGAACAAGATGATCTCACCAACCAGTGGCTGGAATGGGAGGCAACA GAACTGCAGCCAGTTCTGTCTGCTGCCCTACACTGTCTAGTGGTTCAAGGCAAGAAAGGGGAAGATATAC TTGGCCCACTTCGGAGAGTCCTGACTCACATTGATCACAGCTTGAGTCGTCAAAACTGTCCTTTCCTGGC TGGGGACACAGAATCTCTAGCTGACATTGTTTTGTGGGGAGCACTGTATCCTTTACTGCAAGACCCAGCT TACCTCCCTGAGGAGTTGGGTGCCCTGCAAAGTTGGTTCCAGACACTGAGTACCCAGGAACCGTGTCAGC GAGCTGCAGAGACGGTGCTAAAACAGCAGGGTGTCCTGGCACTTCGTCTGTACCTCCAGAAACAGCCACA GCCTCAGCCCCCGCCTCCTGAGGGGAGAACTGTCAGCAACGAGCTGGAGGAAGAGGAACTGGCTACCTTG TCTGAGGAGGACATCGTTACAGCTGTTGCCGCGTGGGAGAAGGGTCTGGAAAGCCTGCCTCCGCTAAAGC TCCAGCAGCATCCAGTGTTGCCTGTGCCTGGAGAGAGGAATGTTCTCATCACCAGTGCCCTCCCCTATGT CAACAATGTCCCCCACCTTGGAAACATCATTGGCTGTGTGCTCAGTGCTGATGTCTTTGCAAGGTATTGT CGCCTTCGCCAGTGGAATACCCTCTATCTGTGTGGTACAGATGAGTATGGTACTGCGACAGAGACCAAGG CCATGGAGGAGGGCCTAACCCCACGGGAAATCTGTGACAAGTACCATGCCATCCATGCTGACATCTACCG CTGGTTCGGCATATCGTTCGATACTTTCGGGCGCACTACCACTCCTCAGCAGACCAAAATCACCCAGGAC ATCTTCCAGAGGTTGCTGACCCGGGGGTTTGTGCTGCGAGATACTGTGGAGCAGCTTCGGTGTGAGCGGT GTGCACGTTTCCTGGCTGACCGCTTTGTGGAGGGTGTGTGTCCCTTCTGTGGCTATGAAGAGGCCCGAGG TGACCAGTGTGACAGGTGTGGCAAGCTCATCAATGCCATTGAGCTCAAGAAACCACAGTGCAAAATCTGC CGCTCCTGCCCTGTGGTGAGGTCCTCACAGCACCTGTTTCTAGACTTGCCTAAGTTGGAAAAGCGTCTGG AGGACTGGTTGGGGAAGACAGTGCCTGGCAGTGACTGGACACCCAATGCCAGGTTCATTATACGTTCCTG GCTTCGAGATGGCCTCAAGCCACGATGCATCACCAGAGACCTCAAATGGGGAACGCCTGTGCCCTTGGAA GGTTTTGAGGACAAGGTATTTTACGTCTGGTTTGATGCTACTATTGGCTACGTGTCCATCACAGCCAACT ACACAGACCAATGGGAGAAATGGTGGAAGAACCCAGAACAAGTGGACCTTTACCAGTTCATGGCCAAAGA CAATGTTCCCTTCCATGGCTTGGTCTTTCCGTGTTCAGTCCTAGGAGCTGAGGACAACTACACCCTGGTC AAGCACATCATTGCTACAGAGTACCTGAACTATGAGGATGGGAAATTCTCTAAGAGCCGGGGCATAGGAG TGTTTGGAGACATGGCCAAGGATACAGGAATCCCTGCTGACATCTGGCGATTCTATCTGCTATACATTCG GCCTGAGGGCCAGGACAGTGCCTTCTCCTGGACAGACTTGTTGATTAAAAACAATTCTGAGCTGCTCAAC AACCTGGGCAACTTCATCAACAGAGCTGGCATGTTTGTTTCTAAGTTTTTTGGCGGTTGTGTGCCTGAGA TGGCGCTAACCCCTGATGACAGACGCCTGGTGGCCCATGTCTCTTGGGAACTCCAGCACTATCACCAGCT GTTGGAAAAGGTTCGGATCCGGGATGCCTTGCGCAGTATCCTCACCATATCTCGCCATGGCAACCAATAC ATTCAAGTGAATGAGCCCTGGAAACGGATTAAAGGTGGTGAGATGGACAGGCAGCGGGCAGGCACAGTGA CAGGCATGGCAGTGAACATGGCTGCCTTGCTGTCTGTCATGCTGCAGCCATACATGCCCACAGTCAGCTC TACCATCCAGACCCAGCTGCAGCTCCCACCTGCAGCCTGCCGCATCCTTGCCACAAGCTTCATTTGTACC TTGCCAGCAGGCCACCGAATTGGCACAGTCAGTCCTTTGTTCCAAAAACTGGAAAATGACCAGATTGAAA ATTTGAGGCAGCGCTTTGGAGGGGGTCAGGCTAAAGGCTCCCCCAAGCCAGCAGCTGTGGAGGCAGTTAC AGCAGCAGGCTCGCAGCACATACAAACGCTGACGGATGAGGTGACCAAGCAGGGCAACGTCGTCCGGGAA CTGAAAGCACAGAAGGCAGACAAGAACCAGGTTGCTGCAGAGGTGGCTAAACTCTTGGATCTAAAGAAAC AGTTGGCTTTGGCTGAGGGGAAACCCATTGAAACTCCTAAAGGCAAGAAGAAAAAGTGAGAGGCTGTTGA CACAAAGTCACTTTACTGGACATGGACAGTAATAAACAATGTACCGTCTATGTGCAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416029 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416029 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006165 same experiment
	EMAGE:29747 same embryo
	EMAGE:29581 same embryo
	EMAGE:31540 same embryo
	EMAGE:29752 same embryo
	EMAGE:29578 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source