EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29919

Heph hephaestin ( MGI:1332240)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005638_01	euxassay_005638_02	euxassay_005638_03	euxassay_005638_04	euxassay_005638_05

euxassay_005638_06	euxassay_005638_07	euxassay_005638_08	euxassay_005638_09	euxassay_005638_10

euxassay_005638_11	euxassay_005638_12	euxassay_005638_13	euxassay_005638_14	euxassay_005638_15

euxassay_005638_16	euxassay_005638_17	euxassay_005638_18	euxassay_005638_19	euxassay_005638_20

euxassay_005638_21	euxassay_005638_22	euxassay_005638_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5557
Entity Detected:	Heph, hephaestin ( MGI:1332240)
Sequence:	sense strand is shown >T5557 GTAGAGCTGGAGTGAAGAAGACAGTCTGCTCTCAGTGGAGATCTCCCCAAGGCAAAGTAAGGACACCACG TACTAGATTATCATCTTACCTGTACTCTTCGTGAGTTTGGGGAGTCTGGTAAATAAGCTGAAGGCATAAC TGCTTGTACTTCTTTGAGAAGGAAGTTTGAACGAAGTCCAGTGTACACGCAGAAGTCCACTGAAAGAAGG TCACTCCTTCCAGGCTGAGGGTCCTAAGGAATGGATAAATGTGTTTCCAGGCATAGTATTGTGTGTAGTT ACATGTGACTGTACCTGTGTTTCACCAGTAAATATTCCATTTCCTGTTTCTCAAAGTACTGTGGGCCATG AAGGCAGGCCATCTTCTCTGGGCTTTACTGTTGATGCACTCCTTGTGGTCTATACCAACTGATGGGGCCA TTCGAAACTACTACTTGGGCATCCAGGATATGCAGTGGAACTATGCTCCCAAAGGAAGAAATGTCATCAC AAATCAGACTCTCAACAATGACACAGTGGCTTCCAGTTTCCTGAAGTCTGGCAAAAACAGGATAGGGAGT AGTTACAAGAAGACTGTTTATAAGGAATACAGTGATGGCACATACACTGAAGAAATAGCCAAGCCTGCCT GGTTGGGCTTCCTAGGACCACTGTTACAGGCTGAGGTGGGGGATGTCATCTTGATTCACCTGAAGAATTT TGCGAGCCGACCTTACACCATTCACCCTCACGGTGTTTTTTATGAGAAGGACTCAGAAGGCTCACTATAC CCAGATGGTTCTTCTGGGTATCTGAAAGCGGATGATTCTGTCCCCCCTGGGGGCAGCCATGTCTACAACT GGAGTATTCCAGAAAGTCATGCCCCCACTGAGGCAGACCCAGCATGCCTCACCTGGATTTACCACTCGCA TGTAGATGCTCCAAGAGACATTGCAACTGGTCTCATTGGACCTCTTATCACCTGTAAAAGAGGGACCCTG GATGGTAATTCCCCACCTCAGAGGAAGGATGTGGACCATAATTTCTTCCTCCTCTTCAGTGTGATAGATG AGAACCTTAGCTGGCACCTTGATGACAACATTGCTACTTACTGCTCAGACCCTGCCTCGGTGGACAAAGA AGATGGAGCCTTTCAAGACAGCAACAGGATGCATGCAATCAATGGGTTTGTCTTTGGGAACTTACCAGAG TTGAGCATGTGTGCACAGAAGCATGTGGCCTGGCACTTGTTTGGCATGGGCAATGAAATAGATGTCCACA CAGCTTTCTTCCATGGACAGATGCTGAGTATCCGTGGACACCACACTGATGTTGCAAACATTTTTCCAGC TACCTTTGTGACTGCTGAGATGGTGCCCCAAAAGTCTGGAACCTGGCTAATTAGCTGTGAAGTGAACAGC CACTTGAGAAGTGGCATGCAGGCCTTCTACAAGGTTGACTCTTGCTCCATGGACCCACCTGTGGACCAGC TCACTGGCAAAGTTCGTCAGTACTTCATTCAGGCCCATGAGATTCAATGGGACTATGGTCCAATAGGGTA TGATGGCAGAACTGGGAAGAGTTTGAGAGAGCCAGGAAGTGGCCCAGATAAGTACTTCCAGAAGAGCTCT AGTCGAATTGGAGGTACTTACTGGAAAGTTCGATATGAAGCCTTTCAAGATGAGACATTCCAGGAAAGGG TACATCAGGAAGAAGAAACACATCTTGGAATACTGGGACCAGTGATAAGGGCTGAAGTGGGTGACACCAT CCAGGTGGTCTTCTATAACCGTGCCTCCCAGCCATTCAGCATACAGCCCCATGGTGTCTTTTATGAGAAA AACTCTGAGGGCACCGTGTACAATGATGGCACATCTCATCCCAAAGTAGCCAAGTCATTTGAAAAAGTCA CATACTACTGGACGGTTCCTCCCCATGCTGGGCCCACTGCTCAGGATCCTGCCTGTCTAACCTGGATGTA CTTCTCTGCTGCAGATCCCACAAGAGATACAAATTCTGGCCTGGTGGGCCCTCTACTGGTGTGCAAGGCT GGGGCCTTGGGTGCAGATGGCAAGCAGAAAGGAGTGGATAAAGAATTTTTTCTCCTCTTCACTGTGTTTG ATGAGAATGAGAGCTGGTACAACAGTGCCAATCAGGCAGCTGGTATGTTGGATTCCCGACTGCTCTCAGA GGATGTCGAGGGCTTCCAGGACTCCAATCGAATGCATGCTATTAATGGATTTCTGTTCTCTAACCTGCCC AGGCTGGACATGTGCAAGGGTGATACTGTGGCCTGGCACCTGCTTGGCCTGGGCACAGAGACTGATGTAC ATGGGGTAATGTTCGAGGGCAACACTGTGCAGCTTCAGGGCATGAGGAAAGGTGCAGTCATGCTCTTTCC TCACACCTTTGTGACGGCCATCATGCAGCCTGACAATCCTGGAATATTTGAAATCTACTGCCAAGCAGGC AGCCACCGAGAGGAAGGGATGCAGGCAATTTATAATGTCTCTCAGTGTTCTAGTCATCAAGACAGCCCAC GCCAACACTACCAAGCTTCAAGAGTCTACTATATCATGGCAGAAGAGATAGAGTGGGATTACTGCCCTGA TAGAAGCTGGGAACTGGAATGGCATAACACATCTGAGAAAGACAGCTATGGCCATGTTTTCCTGAGCAAT AAAGATGGGCTCCTGGGTTCCAAATATAAGAAAGCTGTATTCAGGGAATACACTGATGGTACTTTCAGAA TACCTCGGCCAAGGTCTGGACCAGAGGAGCACTTGGGAATCCTGGGTCCACTTATCAGAGGAGAGGTTGG TGATATCTTGACTGTAGTGTTCAAGAATAAGGCCAGTCGACCATATTCTATACATGCCCATGGAGTTCTA GAATCTAACACTGGCGGGCCACAGGCTGCTGAGCCTGGTGAAGTACTTACTTACCAGTGGAACATCCCAG AAAGATCTGGTCCTGGTCCTAGTGACTCTGCTTGTGTTTCCTGGATTTATTATTCTGCAGTGGATCCCAT CAAGGACATGTATAGTGGTCTGGTTGGACCCCTAGTCATCTGCAGAAATGGTATCTTGGAACCCAATGGA GGCCGGAATGATATGGACCGGGAATTTGCCTTGTTGTTTTTGATCTTTGATGAGAACCAATCTTGGTATC TGAAGGAGAATATTGCAACATATGGACCTCAAGAATCAAGTCATGTTAACTTGAAGGATGCCACCTTCCT AGAGAGCAATAAAATGCATGCTATCAATGGGAAACTCTATGCAAACCTCAGGGGTCTTACTGTATACCAA GGAGAACGAGTAGCCTGGTACATGCTAGCCATGGGCCAAGATACTGACATTCACACTGTACACTTCCATG CAGAGAGTTTCCTCTATCAGAATGGGCAAAGTTACAGGGCAGATGTGGTGGATCTCTTCCCAGGAACATT TGAAGTTGTGGAGATGGTAGCCAGCAACCCTGGGACATGGCTGATGCACTGCCATGTGACTGACCATGTT CATGCTGGCATGGAGACCATCTTTACGGTCTTGTCTCATGAAGAACATTTCAGCACTATGACCACTATTA CTAAAGAGATTGGAAAAGTGATTCTAAGGGACATTGGAGGTGACAATGTGAAGATGCTGGGCATGAACAT TCCCATAAAGGATGTAGAGATTCTGTCTTCTGCTTTGATTGCCATATGTGTGCTTCTGTTGCTCATTGCT CTGGCTCTTGGTGGTGTAGTCTGGTACCAGCATCGACAAAGAAAGCTTCGGCGCAACAGGAGGTCCATTC TTGATGATAGCTTCAAGCTTCTCTCTCTCAAGCAATAATAGCTAAAGCCTGAAGATATCTTCAGAAAACA CATCTGGGATGTATCCCATGTTCCCAGAAAGCTGTGTACTAGTTAAAAACTCTTTTGTCAAGGGGTAGGG ATAGTGGAGAGGCAGAGGATGGAATCAGAATTATCTGGGCATTTCCTTATTTATTTACATTGAATTCATA TGTTCTCACCTTTTTAAAAATTTCCATACAACTTGAGAATTAGGTAAGTTCCTTTGTATACAAATTTCAG CAGCTAGGATATGTCACCCTTCAGTACTACAAAGTACAGAAATTCCTAGAATATAATCATTCTCACAGAA TAGATACCAAGAAAAAGCTATCTATTAGGTTTCTAATTCCAGGACCTAGATAGACAAGTACACTTTTAAT TGAGGCCAAATGATGAGCCACAATGGTAACCCATAACTAAATAGAGGACAAAGAACATAAGTATGATGCA AAATGAGAGGTAGGATGTGTAGCAGGATTCCAAATTTTAGCTTAATTGTCTTTGACAATGGACTATTTGG AGAAATGAATGAATAGGTTGTGGCTGTCAACCTCTGGAACCAGAAGTGCCTGTAAGACAGTCCTTCAAGT TTTTACACAGAAGAGAATTTTGAGTGTCCTCTCTATGTTTCAAATAGCAAATCTTGTTCATTAAAATACT TATTAAAAGATTTGAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6474714 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6474714 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005638 same experiment
	EMAGE:30904 same embryo
	EMAGE:29782 same embryo
	EMAGE:29781 same embryo
	EMAGE:29942 same embryo
	EMAGE:29798 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source