EMAGE mouse gene expression spatial database

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EMAGE:30423

Ppp2r4 protein phosphatase 2A, regulatory subunit B (PR 53) ( MGI:1346006)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001946_01	euxassay_001946_02	euxassay_001946_03	euxassay_001946_04	euxassay_001946_05

euxassay_001946_06	euxassay_001946_07	euxassay_001946_08	euxassay_001946_09	euxassay_001946_10

euxassay_001946_11	euxassay_001946_12	euxassay_001946_13	euxassay_001946_14	euxassay_001946_15

euxassay_001946_16	euxassay_001946_17	euxassay_001946_18	euxassay_001946_19	euxassay_001946_20

euxassay_001946_21	euxassay_001946_22	euxassay_001946_23	euxassay_001946_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3979
Entity Detected:	Ppp2r4, protein phosphatase 2A, regulatory subunit B (PR 53) ( MGI:1346006)
Sequence:	sense strand is shown >T3979 AAGAGGTAATCCCACCGGCATGGCTGCCGCCTTCGCGCGAGTGACTGTAATTCTCGGCTTCCGGTCCTAG CCAGTCAGCGCTCATTTCCCTGCTGGAAGCTTGGCGGAGCCGGGGAGAGCTGCGGGGTGAACATTGAGAG GGACCGGCGAGCATCCGCTCCGGCTCCTCACGGAGAAGATGGCCGAGGGCGAGCGGCAGCCGCCGCCAGA TTCTTCAGAAGAGACCCCTCCAACTACTCAGAACTTTATCATTCCAAAAAAGGAGATCCACACAGTTCCA GATATGGGCAAATGGAAGCGCTCTCAGGCATATGCTGACTACATTGGCTTCATCCTTACCCTCAATGAAG GTGTGAAGGGGAAGAAGCTGACCTTCGACTACAAAGTCTCTGAGGCCATCGAGAAGCTGGTGGCACTTCT TGATACGCTGGATAGGTGGATTGATGAAACCCCGCCAGTGGACCAGCCTTCCCGGTTTGGGAACAAAGCC TACAGAACCTGGTATGCCAAACTTGATCAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCCACCCACC TGGCTGCTGCTGTGCCTGAAGTGGCAGTTTACCTGAAGGAGGCTGTGGGGAACTCCACACGAATTGACTA TGGCACAGGGCATGAGGCTGCCTTTGCTGCTTTCCTCTGTTGTCTCTGCAAGATTGGTGTACTCCGGGTG GACGACCAGGTGGCTATTGTCTTCAAGGTGTTTGATAGGTATCTTGAGGTTATGCGGAAGTTGCAGAAGA CATACAGGATGGAGCCTGCAGGCAGCCAGGGCGTATGGGGTCTGGATGACTTCCAGTTCCTGCCCTTCAT CTGGGGCAGCTCACAGCTCATAGACCACCCCCACCTGGAGCCCAGACATTTCGTGGATGAGAAGGCGGTG AGCGAGAACCACAAGGACTACATGTTTCTCCAGTGCATCCTGTTCATCACTGAGATGAAGACTGGCCCCT TTGCGGAACACTCCAACCAGCTGTGGAACATCAGTGCTGTCCCCTCCTGGTCTAAAGTGAACCAGGGCCT CATTCGAATGTATAAGGCAGAGTGCCTGGAGAAGTTCCCTGTGATCCAGCACTTCAAGTTCGGGAGCCTG CTGCCCATCCATCCCGTCACATCAGGCTAGGAGAGGCTGAACTACCAGAGCCACCCTGGCCAGGTTCCTG TGCTCTCTGTGACCCAGCACCCCTCCCGCCCACCTTCTGTTCTTTCTGTTTGATGAGAGGCTGTTTACTG AGATGGGTGCTAAGCAGGGCCTGAGTTGGGGGAATTGACCACAGTGTGCCCAGTTTTTGACTGTCCTCCG TCTGGTGGCCGAGTAAGAAGAGAGGGGCCTGGACGTGGTCCCCGGCCCTGCCCATCCCTGTGCTCCTGCT TCCTCCCACGGTCAGGCCCATGTGGAGGAGACAAGGACACCCTGCTCTTGTCATTGCTCCCCCTTTCCTC CCCATGATGTAATGGTTCCCTCATTAAGAGCAGGAGTTCCGTGCCTCCAGGGCCGGCTGGTGGGTTCCCC GCTAGCTGTGTTCCACTGGGTATCTGCTATGGAGCTGAAGGGTGAGGCTGTCATCAGCTGTTGGGAGGGC TGGGGTTGGGGGAGGTTGGTTTGTAGGGGCCATGGATGATGAGGCTGGTCTTCTCAAGGTACCATCCTAG GCCCTGGGGGACCTGTGTCCATCCTCATGCTGGCTTTGTTCCCTGGCCCTTGGCTTTGCTAGGTTTCCTG ACTGTTAACATAACACTCCCCTTCCTGGGGATAGCCTAGCTATGGAAGCTGCCCTGGGCTCCTGGGAAAG CCCATTGCTTGGGTGAGATCTGACTTTCCAGAGCTGTCAAGCAGGGAGAGGAAGAGACCCCGGGAGGCAG TAGGACTGTTGTGTCTGTGGAGAACCTAGTTTTCTCCAGTCAGTCTCAGGCCTGCTGATTCTGCTGCCTC TGCCTGCTGAGTGTCTGAAGCTTCCCCCGAGGCCTTGGCTTGCTGCTCCGCTCCCTTGGGTCGGTTTGTG GCTCGTGTGGGAGAGCAAAGAAGGTAGCAGCCCCCTCCTCCCACTTCCCTCTGTGAGATGAGGGAAGTGC TGGCCTGGGCCTAGGCATAGACTCAGAATGGGTAGGGCTGCTAAGGGGACCTAGCCACAGTCACCAGTTT TCTCTCAGCGTGTGGCTGAACTGGAACCTGCAGGCTTTCCTGTCCCACTTGGGCCTGCCCTTCTTCACTG CCCATCCTGTGTTTGTGCCCGCTTAGCTTTGTGGAGTGGACACTGAGAAGCACAACTCAGGGGCTTGGCC AGGAATTAACTATGGTCTGGGCAGAGCGGGGATTGAAGTCATCAGAGTCACAGGCAGTTCCCCAGGAAAA GCCCAGTGCCAGCTTCCTTCCAGGGCCAGGCCACTGCCTTCCTGGCTCCCTTCCCTGTCCCTGCGTCCCC AGCCCTGCGCCTTCCTTGCTATTTGGATTAAAGCCTCTGTTTTACACCTGAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30058414 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30058414 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001946 same experiment
	EMAGE:29507 same embryo
	EMAGE:30421 same embryo
	EMAGE:30396 same embryo
	EMAGE:30400 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source