EMAGE mouse gene expression spatial database

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EMAGE:30638

Mylk myosin, light polypeptide kinase ( MGI:894806)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004987_01	euxassay_004987_02	euxassay_004987_03	euxassay_004987_04	euxassay_004987_05

euxassay_004987_06	euxassay_004987_07	euxassay_004987_08	euxassay_004987_09	euxassay_004987_10

euxassay_004987_11	euxassay_004987_12	euxassay_004987_13	euxassay_004987_14	euxassay_004987_15

euxassay_004987_16	euxassay_004987_17	euxassay_004987_18	euxassay_004987_19	euxassay_004987_20

euxassay_004987_21	euxassay_004987_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8039
Entity Detected:	Mylk, myosin, light polypeptide kinase ( MGI:894806)
Sequence:	sense strand is shown >T8039 AAGAGGTAATCCAGAGGGGAACTCGTGTCTCTGCCTGGGTTTCCAAGAGAGCCTTTATGTGAAAGCATGT CTGGCCAGTTACCTGGGAAGACCATGGGGGACGTGAAACTGTTTGCCTCGTCACACATGTCTAAAACCTC CCACAGTGTGGATCCCTCGAAGGTCAGCTCCATGCCTCTCACCGAGGCCCCAGCTTTCATTCTGCCCCCT CGGAACCTCTGCGTCAAAGAAGGAGCCACAGCCAAGTTCGAAGGGAGGGTCCGGGGCTACCCAGAGCCCC AGGTGACATGGCACAGAAAAGGGCAAGCCATCACCAATGGTGGCCGCTTCCTGCTGGACTGTGGAGTGCG GGGGACTTTTAGCCTTGTCATCCACACTGTCCGCGAGGAGGACAAGGGCAAATACACCTGTGAAGCTAGC AATGGCAGTGGCGCCCGCCAGGTGACCGTGGAGCTGACTGTGGAAGGGAATTCTATGAAGAAGCGTGATC AGCCTGTTCTTTCTAAAGCCTCAGGATTTCCAGGGGAGACTCGTCCTAGCATCTGGGGTGAGTGCCCACC AAAGTTTGCCACCAAGCTGGGCAGAGCTGTGGTCAAAGAAGGGCAGATGTGGCGATTCTCCTGCAAGATC ACCGGCCGGCCTCCACCACAGGTCACCTGGCTCAAGGGAAATGTCCCGTTGCAGCCAAGTGCCCGCGTGT CAATGTCAGAGAAGAATGGCATGCAGATCCTGGAGATCCGTGGGGTCACTCGGGATGACCTGGGCGTGTA CACCTGCATGGTGGTGAATGGGTCAGGGAAAGCCTCCATGTCGGCGGAGCTCTCCATCCCAGGTTTGGAC AATGCCTCTAGGTTGGCTGTGAGAGGAACCAAAGCCCCCAGTCCAGACATCAGGAAGGAGGTGACCAATG GAGTCTCCAAAGACCCAGAGACTGTGGCTGAAAGTAAGAACTGTCCAAGTCCCCAGAGGAGTGGCTCCTC AGCCCGGGCCACCAACAGTCACCTCAAGTCCCCGCAGGAGCCCAAGCCAAAGCTGTGTGAGGATGCTCCC AGAAAGGTCCCGCAGTCCTCCATCCTGCAGAAGTCCACCAGCACCATCACTCTGCAGGCCTTGAAAGTCC AGCCAGAAGCGAGAGTACCAGCCATAGGGTCCTTCTCTCCTGGGGAAGACAGGAAGAGTCTGGCTGCCCC TCAGCAAGCCACACTCCCTACCAGACAGTCTAGCCTGGGAGGTTCCGTTGGGAACAAGTTTGTTACCGGG AACATCCCCAGAGAGAGCCAAAGGGAGTCAACATTCCCCAGATTTGAGAGTCAGCCCCAAAGCCAGGAGG TCACTGAAGGTCAGACAGTCAAGTTCATCTGCGAGGTTTCAGGTATACCAAAGCCTGACGTGGGCTGGTT CCTGGAAGGCATCCCCGTGAGGAGACGGGAAGGCATCACTGAGGTTTATGAAGATGGTGTATCCCATCAC CTGTGCCTGCTGAGAGCCCGGACGAGAGACAGTAGGAAGTACAGCTGCACAGCATCCAATAGCCTTGGCC AGGTGTCCTGCAGCTGGAGCCTGCTGGTGGACCGGCCGAACTTGGCACAGACAGCCCCTTCATTCTCTAG TGTTCTGAAGGACAGTGTTGTCATTGAGGGCCAGGATTTTGTGCTACGGTGCTCAGTGCAGGGGACTCCA GCACCCCGGGTCACGTGGCTGCTCAATGGACAGCCCATCCAGTTTGCTCACTCCATCTGTGAGGCAGGCG TGGCCGAGCTCCACATCCAGGATGCCCTGCCAGAGGACCGTGGCACCTACACCTGCCTGGCTGAGAATGC CATGGGACAGGTGTCCTGCAGCGCCACTGTCACCGTCCAAGAGAAGAAAGGTGAAGGAGAGAGGGAACAC CGTCTGTCCCCAGCTCGCAGCAAGCCCATCGCACCCATCTTCCTGCAGGGCCTCTCCGACCTCAAAGTCA TGGATGGAAGCCAGGTCACTATGACAGTCCAGGTGTCAGGGAACCCACCTCCGGAAGTCATCTGGCTTCA CGACGGGAATGAGATTCAGGAGTCCGAGGACTTCCACTTCGAGCAGAAGGGTGGCTGGCACAGCCTGTGT ATCCAGGAGGTGTTCCCGGAGGACACGGGCACGTACACCTGTGAGGCCTGGAACAGTGCCGGGGAGGTCC GCACCCGGGCCGTCCTCACCGTGCAAGAGCCTCATGATGGCACCCAGCCCTGGTTCATCAGCAAGCCTCG TTCGGTGACAGCCACCCTGGGCCAGAGTGTCCTCATCTCCTGCGCCATAGCTGGAGACCCCTTTCCCACC GTGCACTGGCTCCGGGATGGCAGAGCCCTCTCCAAGGACAGTGGCCACTTTGAGCTGCTCCAGAATGAGG ACGTGTTCACCCTGGTTCTAAAGAACGTGCAGCCCTGGCATGCCGGCCAGTATGAGATTCTGCTCAAGAA CCGGGTTGGCGAGTGTAGTTGCCAAGTGTCACTGATGCTTCACAACAGCCCTTCCAGAGCCCCACCACGG GGGCGGGAGCCTGCCAGCTGCGAGGGCCTCTGTGGTGGTGGAGGAGTTGGTGCTCATGGTGATGGTGACC GTCATGGGACCCTGAGGCCCTGCTGGCCAGCAAGAGGGCAAGGTTGGCCTGAGGAGGAAGACGGAGAAGA CGTGAGGGGGCTGTTGAAGAGACGGGTGGAGACCAGGCTGCACACAGAAGAGGCCATCCGCCAGCAGGAG GTGGGGCAGCTGGATTTTCGAGACCTCCTGGGAAAGAAGGTGAGCACCAAGACCGTCTCAGAAGACGACC TGAAGGACATCCCAGCCGAGCAGATGGATTTCCGCGCCAACCTTCAGCGGCAAGTGAAGCCAAAGACCAT ATCCGAGGAAGAGAGGAAGGTGCATAGCCCTCAGCAGGTTGACTTCCGGTCTGTCCTCGCCAAGAAGGGG ACCCCTAAGACCCCCGTTCCTGAGAAGGCACCTCCAAAAGCTGCCACCCCAGACTTTCGCTCTGTGCTAG GTGGCAAGAAGAAGTCACCCTCGGAGAATGGGGGCAACAGCGCTGAGGTCTTGAATGTCAAGGCTGGGGA AAGCCCCACACCTGCAGGCGATGCGCAGGCCATCGGGGCCCTGAAACCCGTAGGCAATGCCAAGCCTGCT GAGACCCCGAAACCTATAGGTAATGCCAAGCCTACCGAGACCCTGAAGCCTGTGGGCAACACCAAGCCTG CCGAGACCCTGAAACCCATAGCCAACGCACAGCCTTCAGGGTCCCTGAAACCTGTAACCAACGCACAACC TGCTGAGCCCCAGAAGCCTGTGGGCAATGCCAAGTCTGCTGAGACCTCGAAACCAGCTGGGAAAGAAGAA GTCAAGGAGGTAAAGAATGATGTGAACTGTAAGAAAGGGCAGGTCGGAGCCACGGGCAATGAAAAGCGAC CCGAGAGCCAAGGCTCGGCCCCAGTTTTCAAGGAAAAGCTACAAGATGTCCATGTGGCAGAGGGGGAGAA GCTGCTACTCCAATGCCAGGTGATCTCTGATCCCCCCGCCACTGTCACCTGGTCGCTGAATGGGAAGACA CTCAAGACCACAAAATTCATCGTCCTCGCCCAAGAAGGCTCACGTTTCTCTGTCTCCATCGAGAAGGCAC TGCCTGAGGACCGAGGTTTGTACAAGTGTGTAGCCAAGAACAGCGCTGGCCAGGCAGAGTGTTCCTGCCA AGTCACTGTGGACGATGCTCAGACCAGTGAGAACACCAAGGCCCCGGAGATGAAATCCCGGAGACCCAAG AGCTCTCTTCCCCCTGTGCTAGGAACAGAGAGCGACGCAACTGTGAAAAAGAAACCTGCCCCCAAGACAC CCACAAAAGCAGCTATGCCCCCTCAAATCATCCAGTTCCCCGAGGACCAGAAGGTGCGCGCAGGAGAACC TGTGGAGCTGTTTGGGAAAGTGGCTGGCACCCAGCCCATCACCTGCAAGTGGATGAAGTTCCGAAAGCAG ATCCAGGAGAGCGAGCATATCAAGGTGGAGAATGGCGAGAGCGGCAGCAAGCTCACCATCCTGGCCGCGC GGCAGGAGCACTGTGGCTGCTACACACTGGTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAAGTCAA CCTCACGGTCGTGGATAAGCCAGACCCCCCGGCTGGCACACCTTGTGCTTCTGACATTCGGAGTTCCTCA CTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAATGTGGAGATCT GGGACACAGAGGACAAGGTGTGGAAAGAGCTAGCCACGTGCCGCAGCACCTCTTTTAATGTCCAAGACCT GCTGCCTGACCGCGAGTACAAATTCCGAGTGCGTGCAGTCAACGTCTATGGAACCAGCGAGCCAAGCCAA GAGTCCGAGCTCACAGCTGTGGGAGAGAAACCTGAAGAGCCAAAGGATGAAGTGGAAGTGTCCGATGATG ACGAAAAGGAACCTGAGGTTGATTACCGGACGGTGACTGTTAACACTGAACAGAAAGTGTCTGATGTGTA TGACATTGAAGAGCGACTAGGATCTGGGAAGTTTGGACAAGTCTTCCGACTTGTGGAAAAGAAAACTGGA AAAATCTGGGCAGGGAAATTTTTCAAGGCCTATTCCGCCAAGGAGAAAGACAACATCCGTCAGGAGATCA GCATCATGAACTGCCTCCACCACCCCAAGCTGGTCCAGTGTGTGGATGCCTTCGAAGAAAAGGCCAACAT TGTCATGGTTCTGGAGATCGTGTCCGGGGGTGAGCTGTTTGAGCGTATCATTGACGAGGACTTCGAGCTG ACGGAGCGGGAGTGTATCAAGTACATGAGGCAGATCTCGGAAGGGGTGGAGTACATCCACAAGCAGGGCA TTGTGCACCTGGACCTCAAGCCCGAGAACATCATGTGTGTCAACAAGACAGGCACCAGGATCAAGCTCAT TGACTTCGGCCTGGCACGGAGACTCGAGAACGCTGGGTCTCTGAAGGTCCTCTTTGGGACCCCAGAGTTT GTGGCTCCTGAAGTGATCAACCATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGTCATCT GCTATATTCTGGTCAGCGGACTTTCTCCCTTCATGGGTGACAATGATAACGAGACCTTAGCCAACGTCAC TTCAGCCACCTGGGACTTCGATGACGAGGCGTTCGATGAGATCTCCGATGACGCAAAGGATTTCATCAGT AACTTGCTGAAGAAAGATATGAAAAACCGTCTGGACTGCACCCAGTGCCTTCAGCACCCATGGCTGATGA AAGACACCAAGAACATGGAGGCCAAGAAGCTGTCCAAGGACCGGATGAAGAAATACATGGCAAGAAGGAA GTGGCAGAAAACGGGCAATGCTGTGAGAGCCATCGGGAGACTGTCCTCTATGGCAATGATCTCAGGGCTC AGTGGCAGGAAATCTTCAACAGGGTCACCAACCAGCCCAATCAATGCGGAGAAACTAGAGTCTGAAGACG ATGTCTCCCAAGCTTTCCTGGAGGCTGTTGCTGAGGAAAAGCCCCATGTGAAACCTTATTTCTCTAAGAC CATCCGTGATCTGGAGGTCGTGGAGGGAAGCGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGAC CCTGAGGTGGTCTGGTTCAAGGATGACCAATCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGACG AAGACGGTAACTGCTCGCTCATCATCAGCGACGTCTGTGGGGATGATGACGCCAAGTACACCTGCAAGGC TGTCAACAGCCTCGGAGAAGCCACCTGCACTGCGGAGCTCATCGTGGAGACCATGGAGGAAGGGGAAGGG GAGGAGGGGGGAGAGGAGGAGGAGGAGGAGGAGTGAAGCCAAGCCAAACAGAAGCAGTTCCTAAGTCATT TTAAAAAGGACTAATTCCTTAAAGCTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30056142 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30056142 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004987 same experiment
	EMAGE:32011 same embryo
	EMAGE:31949 same embryo
	EMAGE:31058 same embryo
	EMAGE:31650 same embryo
	EMAGE:31973 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source