EMAGE mouse gene expression spatial database

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EMAGE:30744

Sdha succinate dehydrogenase complex, subunit A, flavoprotein (Fp) ( MGI:1914195)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003237_01	euxassay_003237_02	euxassay_003237_03	euxassay_003237_04	euxassay_003237_05

euxassay_003237_06	euxassay_003237_07	euxassay_003237_08	euxassay_003237_09	euxassay_003237_10

euxassay_003237_11	euxassay_003237_12	euxassay_003237_13	euxassay_003237_14	euxassay_003237_15

euxassay_003237_16	euxassay_003237_17	euxassay_003237_18	euxassay_003237_19	euxassay_003237_20

euxassay_003237_21	euxassay_003237_22	euxassay_003237_23	euxassay_003237_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4856
Entity Detected:	Sdha, succinate dehydrogenase complex, subunit A, flavoprotein (Fp) ( MGI:1914195)
Sequence:	sense strand is shown >T4856 CGGACGCGTGGGCGGACGCGTGGGTTGCGGGAAGGCAGACATGGCCGGGGTTGGCGCAGTTTCGAGGCTT CTTCGCGGGCGGCGCTTGGCGTTAACTGGGGCGTGGCCAGGAACACTCCAAAAACAGACCTGCGGCTTTC ACTTCTCTGTTGGTGAGAACAAGAAGGCATCAGCTAAAGTTTCAGATGCAATTTCTACTCAATACCCAGT GGTGGATCATGAATTTGATGCTGTGGTTGTAGGCGCTGGAGGGGCAGGCTTGCGAGCTGCATTTGGCCTT TCTGAGGCAGGGTTTAATACTGCATGCCTTACAAAGCTCTTTCCTACCCGATCACATACTGTTGCAGCAC AGGGAGGTATCAATGCTGCTCTGGGGAACATGGAAGAGGACAACTGGAGATGGCATTTCTATGACACTGT GAAAGGCTCCGACTGGCTGGGGGATCAGGATGCCATCCATTACATGACAGAGCAAGCTCCTGCCTCTGTG GTTGAGCTAGAAAATTATGGTATGCCATTTAGCAGAACTGAAGATGGGAAGATTTATCAGCGTGCATTTG GTGGACAGAGCCTCAAGTTTGGGAAAGGCGGGCAGGCTCATCGGTGTTGCTGTGTGGCTGATCGGACAGG CCACTCACTCTTACACACGTTGTATGGAAGATCTCTGCGATATGACACCAGTTATTTTGTGGAATATTTT GCACTGGATCTTCTGATGGAAAATGGGGAGTGCCGTGGTGTCATTGCACTGTGCATAGAAGATGGGTCCA TACACCGAATAAGAGCAAAAAACACTGTTATTGCTACTGGGGGCTACGGGCGAACCTACTTCAGCTGTAC ATCTGCCCATACCAGCACAGGGGACGGCACAGCCATGGTCACTAGGGCTGGTTTGCCTTGCCAGGACTTA GAATTTGTTCAGTTCCACCCCACAGGTATATACGGTGCTGGCTGCCTCATCACAGAAGGGTGTCGTGGAG AGGGGGGGATTCTCATCAACAGTCAAGGTGAAAGGTTCATGGAGAGATACGCACCTGTTGCCAAGGACCT GGCATCAAGAGATGTTGTGTCTCGATCCATGACTCTTGAGATCCGTGAAGGAAGAGGCTGTGGCCCTGAG AAAGATCACGTCTACCTGCAGTTGCATCATCTGCCTCCTGAGCAACTGGCCACACGTCTGCCCGGAATTT CAGAAACGGCCATGATCTTCGCTGGTGTGGATGTCACTAAGGAGCCCATCCCAGTCCTCCCCACTGTGCA TTACAACATGGGTGGGATTCCCACTAACTACAAGGGACAGGTGCTGAAGCATGTGAATGGCCAGGATCAG ATTGTGCCTGGTCTGTATGCCTGTGGGGAGGCTGCCTGCGCCTCAGTGCATGGTGCCAACCGGCTTGGAG CAAATTCTCTCTTGGACCTTGTAGTCTTTGGCAGAGCCTGTGCCCTGAGCATTGCAGAATCTTGCAGGCC TGGAGATAAAGTTCCTTCAATTAAGGCAAATGCTGGAGAAGAATCGGTTATGAATCTTGACAAGTTGAGA TTTGCCGATGGAAGTATAAGAACATCAGAACTGCGCCTAAACATGCAGAAGTCGATGCAGAACCATGCTG CAGTGTTCCGTGTGGGAAGTGTATTGCAAGAAGGCTGTGAAAAAATCAGTCAGCTCTATGGAGACCTAAA GCATCTAAAGACATTTGACAGGGGAATGGTTTGGAACACAGACCTGGTGGAGACCCTGGAGCTGCAGAAT CTGATGCTGTGCGCACTGCAGACCATATATGGTGCAGAAGCTCGGAAGGAGTCCCGGGGAGCCCATGCCA GGGAAGATTACAAAGTGCGGGTCGATGAGTATGATTACTCCAAGCCCATCCAGGGACAGCAGAAGAAGCC GTTTGGGGAACACTGGAGGAAGCACACGCTCTCATATGTGGACATCAAGACTGGCAAGGTTACTTTGGAG TACAGACCTGTTATCGACAAGACCTTGAATGAGGCTGACTGTGCCACCGTACCTCCTGCTATCCGTTCCT ACTGATGAAACCTGACAGCTACAGGACCAGCTTATGTGATTATATCATAGCTTACCTGCGTTTCCCCTCA TACTTGTCTTGTTAAAATTCTGCTCTCATGAACAAGGAGTCACTTCATAGATTGTGATCAACAGCTTGGT AGTACTTGCTGTAAGGGATTGAGTTGCGCCACACTTTGTCTCTTTATTCTTGTGCAGTGATAAAACTGGC ATAATTCTTAAGTGAAGTACAAATGAGCAATCTTTTATTTCCAAATAAAACCATGTAGTAGTGGATTTTA GTTCTATCTATTGGTCTGAAATAGCTTCCCCTGTCTAGCTGCAGCACAAGTATTGTTTTGTTCATTCTCT GTGTGAAGTAGGGCAGGTCCTTCATAATTTGAAGGAAAGTTTGGATATGGTAAGTATCGAGCCAGTGCCT TGTTTAGGTTCCTGGTGGGTAGAGGTATATGCATATGTCCATGTTTATTTGAGGGAGTATGTGCTGGCTA GCTTTGTCAACTTGACAAAAATCTAGCATCACCTAAGAAGAGAGTCTCGATGAAGGATTGCTTCTGTTTG CTTGGTCCATGGGCATGTCTCTGAGGGAATGGCTTGATTAATTTAATTGCTAAGGAAGACAGCCCACTGT GGGCACCATCATTCTCTGGTCAAATGAGTCTGGACTGTATAAGAGCAAGCTGAGCCTTCCCCATGCATGC AGTTATTCATTGTCTACTTCTCACTGTGAATGTAACTAGTTTCTTCAAGTTCATGCCCTGACTTCCTTGA AATGAAGGACTTTAACCAGCAATTGTAAGCCAAATAAACCCTTTCTCAAAGTTAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5067510 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5067510 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003237 same experiment
	EMAGE:32141 same embryo
	EMAGE:32180 same embryo
	EMAGE:32189 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source