EMAGE mouse gene expression spatial database

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EMAGE:30859

Alg2 ( MGI:1914731)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_007102_06	euxassay_007102_07	euxassay_007102_08	euxassay_007102_09	euxassay_007102_10

euxassay_007102_11	euxassay_007102_12	euxassay_007102_13	euxassay_007102_14	euxassay_007102_15

euxassay_007102_16	euxassay_007102_17	euxassay_007102_18	euxassay_007102_19	euxassay_007102_20

euxassay_007102_21	euxassay_007102_22	euxassay_007102_23	euxassay_007102_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8408
Entity Detected:	Alg2, ( MGI:1914731)
Sequence:	sense strand is shown >T8408 GCGGAGAGCGCAAGCGCGGCTCATCTACCATGGCCGAAAACCTGTACCGAGCCCGTTCCCGGGTTTACAG CCCATCTGTGCTGTTTCTGCACCCAGACATGGGTATAGGCGGAGCCGAGCGCCTAGTGCTGGACGCGGCG CTGGCGCTGCAGGAGTACGGCTGTGATGTGAAGATATGGACCGCGCACTACGACCCGAACCACTGCTTCA TCGAGACCCGCGAGCTCTCGGTGCAATGCGCAGGGGACTGGCTGCCTCGCAGCCTGGGCTGGGGCGGCCG CGGCGCCGCCATCTGCTCCTACGTGCGCATGGTCTTTCTGGCGCTCTACGTGCTGTTTCTCTCCGGCGAG GAGTTCGACGTGGTGGTGTGCGACCAGGTGTCTGCCTGTATCCCGGTGTTCAAACTGGCCAGACGGCGTA AGAGGGTCCTATTTTACTGTCACTTCCCAGATCTGCTGCTTACTCAGAGAAATTCAGCTCTGAAGAAGTT CTACAGGGCCCCCATCGACTGGATCGAGGAATACACCACAGGCATGGCAGACCGCATCTTGGTCAACAGC CAGTACACTGCTTCCGTCTTTAAAGAAACCTTCAAGACCCTGTCTCACAGAAATCCTGATGTGCTCTACC CATCTCTGAATATCGGCAGCTTTGACTTGGCTATTCCTGAAAAGATAGATGACCTCGTCCCCAAGGGGAA GCAATTCCTGTTCCTCTCTATCAACCGATACGAAAGGAAGAAAAATCTGCCCTTGGCACTGAGATCCTTG GTGCAGCTTCGGAATCGGTTACCATCTCAAGAGTGGGATAAGGTTCATCTCTTCATGGCCGGTGGTTATG ACGATAGGATCCCGGAGAACGTGGAGCACTATAAGGAGTTGAAGAAAATGGTCCAAGAGTCAGACCTTGA GCGTCATGTGACCTTCCTGCGGTCCTTCTCGGACAGACAGAAGATCTCACTCCTCCACGGCTGCTTGTGT GTGCTCTACACTCCGAGCAACGAGCACTTTGGCATCGTCCCTCTGGAGGCCATGTACATGCAGTGCCCAG TCATCGCTGTTAATAACGGTGGGCCCCTGGAGTCCATTGTCCACAAGGTCACGGGGTTCCTGTGTGAGCC AGACCCAGTGCACTTCTCAGAAGCCATGGAGAAGTTCATCCACAAACCATCCTTAAAAGCCACGATGGGC CTCGCTGGAAAAGCCAGGGTGGCGGAGAAGTTTTCAGCTGATGCATTTGCAGACCAGCTGTACCAGTATG TCACGAAGCTGGTGTAGTCAGGAGAGTACAGTGTAGAGTCTGTATGCTACATGCACTGCGATCCCTCTTA TGGGTTGTAGAAACGCCTTTGAAACCAAAAGAAAGGGGGAAACAAAGGAGCTAGAATCTAGTGCTAGTGA GATTTTCTTTTTTAAGTAGACTTGAGTCTTGAATGTGAGCCACCTGTCCACCACACCTACCTGTCATTTC TGAAATATTCTTAATGCTGTAATCATTCCGTATCTCGTGAGTGTTGTTGAAAGTATGGTAGGAATCTGCT GCTAGCAGACTATTTTAACTCTATTTTCCTGGATTATTGTTCCTTTATATATAAAGGTTTGATCATCCAG TGCCTTAATTGGCTTTAGTAGTGTAGGTCTTACCATTGTCACAGTCAATTGATTTGACTTCAGAGCGTAA TGGGAACGAGGCTGCTGCAGCTCCCAAGCTCGGTTTACTTAAAGTGTTCTCTAGTAGCTGCTAGAAAATT TTTGTTAGAATTTTGGCTGGATCTATAACAAGAGTACTCAGTATTATTTATTGTTTTGGTTTTTGTTTTG TTTTGTTTTTTACTGCAACTTGACAATAAAATATGTTTGGCATCAGAAGAGAATAGAACATTAGATCTGA GCCCGATGCTCTGACTTCATCTTGTTAAATACATAAACCTGAAGTCAGAAGACTAGGATAAATGAGATAG TTTACATGAAGGAACACCATCACACATGTTTTGGCCTACCGTGTCACGCTTGGCCTTATTCCTTTGAGCC TGTGCCAGAACAGTGTATTGAGGCAGGGAGCAAAGACAAAAAGGGTCCCATATCCCTTTCTAGAAAATAT CCCTTGATGACCTGATTCTCTTCCACTAGCCCTCACTACTAAAAGTCCTGTCACCTCCTAGTGTGGCTCT CAGTGTGGGCCGGGGACCAGGCCTCAGGGCACAGGCCTTTGGGAACTGTATTAGAGTTCTCTAAAATAGC AAACAGCACTTAACAGAATGAATTCCCAGAAGAAGAAGAACAAATGGCGTACAGGCTTCTGTCCAGCTAA TCCAACAATGAACAGAAAGTCCAGAAATCCAGTAGTTGTTCAGTCCAGGAGGCTGAATGTCTCAGCTGAT CTTCAGTATACACTGGAATCCCAAAGTAGGCTCTGACGCCGGTGAAGGGATGGACTTGCCAGCAAGGTAA GGACAAGCAAGCCAAGAGCAAACGCTCCCTACTTCCATGTCCTTGTATAGGCTGCCAGCAGAAGGCATGG CGCAGACTAGAGGTACGTTTTCCCACTTGAAGATCAGAATTAAAGGAAGACCTGCCAACTTCAAGTTAAG CAAAAATCCCTCCCAGGTGTGCCCTCCATTGTTAGATTTTAGTTCAATCCAGATGGAGTCAAGTTGACAA CCAAGAATGGCCACCACCGGGGACACTCCGCATCTGAACTATATACTAAGCTTCATATTTCAGACGTGTT TCTTAATGTTGTTGGTGTCTCCAGCCCTCGTAGTGTTTGTATCGTATTCTCTGCAGTTTAGCATGCCAGT GTTTCCTGTCCTTCACGTAAATGCCTTTGTGTGAAGCTTGCTTGACACCCCGCCCCCACCCCACCTCTCA ACTCCCCTATCCCTGTGACACTGCCCAGTAATAACTGTTCGTTGTTGTTTACCTGTTGCTTGTAAGTGCA AGTATTAAAGCAATTTTAAAGCTAAATTCACCTGTAAGAATATAATAAATATCTGTAATCAGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5686826 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686826 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007102 same experiment
	EMAGE:29728 same embryo
	EMAGE:31121 same embryo
	EMAGE:31730 same embryo
	EMAGE:31821 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source