EMAGE mouse gene expression spatial database

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EMAGE:30868

Ccdc3 ( MGI:1921436)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009735_01	euxassay_009735_02	euxassay_009735_03	euxassay_009735_04	euxassay_009735_05

euxassay_009735_06	euxassay_009735_07	euxassay_009735_08	euxassay_009735_09	euxassay_009735_10

euxassay_009735_11	euxassay_009735_12	euxassay_009735_13	euxassay_009735_14	euxassay_009735_15

euxassay_009735_16	euxassay_009735_17	euxassay_009735_18	euxassay_009735_19	euxassay_009735_20

euxassay_009735_21	euxassay_009735_22	euxassay_009735_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain floor plate

strong

regional

strong expression: see section 07 08 09 10 12 13 14 15 16

cerebral cortex mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 13 14 15 16 17 18

spinal cord floor plate

strong

regional

strong expression: see section 10 11 12 13 14

naso-lacrimal duct

strong

regional

strong expression: see section 03 04 05 06 14 15 16 17 18 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 11 12 13 14 moderate expression: see section 05 06 07

medulla oblongata floor plate

strong

regional

strong expression: see section 11

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

metencephalon floor plate

strong

regional

strong expression: see section 11 12

anterior naris epithelium

strong

regional

strong expression: see section 08 09 11 12 13

external naris epithelium

strong

regional

strong expression: see section 08 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30590
Entity Detected:	Ccdc3, ( MGI:1921436)
Sequence:	sense strand is shown >T30590 GCTCATGTGCTCCTCGGTGCAGAAGGCCCTGTTTGAGGAAGAGGACCATGTCAAGAAGCTGCAGCAGAAG GTGGCCACCCTGGAGAAGCGGAACAGGCAGCTCCGAGAGCGGGTCAAGAAGGTCAAGAGGTCTCTGAGGC AGGCACGGAAGAACAGCCGCCACCTGGAGCTGGTGAACCAAAAACTCAATGAGAAGCTAGGGGCCTCCAG TGCTCAGCAGCACATTAACGCTCTGGGCCGGGAGCCCGTGCGTGCCCCCTATCTGCATGGGTAGCAGGGG CAGGTGGATGCTGGCCTGCCTTGCCTGTGCCGGCTGACTGAGAACAACCGCAAGCTCTATATATGCACTT ACTTCAAACATTATAGTAGCTATCCATTTGGCATTTGGTAGAGTCTAACTGGGTAAATTGTCAGGTTTTG CTTTCTTTCCCATTGGGTCCTGGCCTTACTGGCCATTTGACTTCAGATCCCATGTGCCACATGTCCTCTT TCTGTCCTGGTGCCACACAAGAGCCTTAAAACCGGAAGGCAATAGTATCTTGAGTCTGGCCCTCAGCCTC TAGAGAGTTCCTTCGCTGGGGGGAGAACATTCTAGAACCTCGGTATACATGAAGATATCACAGGACTCTC TTGTTTCAGAAGGGAGTCGGTGTTGAGGCTCAGCAACCAGGGTTGGCACACGCATGCGTGTTCTAAAATG TTCTGGAATGTTCTGCCTTTTCTCGTGTTTTTAGAAATGGTGTCTACGTGTAAATGTGCAGGGCACCCAC CCGTGCTTCAGTCACCATTTCTCCCAAAGCTGAAAGATGTTAAGACTTAATGAGTTCATTACAGGTAAGG GAACTGGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5686139), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58400. Forward Primer - name:058400_F_IRAV113_h03_Ccdc3, sequence:GCTCATGTGCTCCTCGGT; Reverse Primer - name:058400_R_SP6_IRAV113_h03_Ccdc3, sequence:CGCCCAGTTCCCTTACC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009735 same experiment
	EMAGE:30010 same embryo
	EMAGE:30013 same embryo
	EMAGE:30875 same embryo
	EMAGE:30027 same embryo
	EMAGE:30015 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source