EMAGE mouse gene expression spatial database

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EMAGE:30896

Supt5h suppressor of Ty 5 homolog (S. cerevisiae) ( MGI:1202400)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004056_01	euxassay_004056_02	euxassay_004056_03	euxassay_004056_04	euxassay_004056_05

euxassay_004056_06	euxassay_004056_07	euxassay_004056_08	euxassay_004056_09	euxassay_004056_10

euxassay_004056_11	euxassay_004056_12	euxassay_004056_13	euxassay_004056_14	euxassay_004056_15

euxassay_004056_16	euxassay_004056_17	euxassay_004056_18	euxassay_004056_19	euxassay_004056_20

euxassay_004056_21	euxassay_004056_22	euxassay_004056_23	euxassay_004056_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 06 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20

cervical ganglion

weak

regional

weak expression: see section 09 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 19 weak expression: see section 07

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 15

spinal cord

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18

thoracic ganglion

weak

regional

weak expression: see section 11 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6158
Entity Detected:	Supt5h, suppressor of Ty 5 homolog (S. cerevisiae) ( MGI:1202400)
Sequence:	sense strand is shown >T6158 GGAAGCTTCAGGTGGCAAGCGATCGCTGCGGAAGATGTCGGACAGCGAAGACAGCAACTTCTCCGAGGAG GAGGACAGCGAGCGCAGCAGCGAGGCGGAGGAGGCCGAGGTTGAGGAAGACCAGCGAAGTGCTGCAGGCA GTGAAAAAGAGGAGGAGCCTGAGGAAGAGGAGGAGGAAGAGGAAGAATACGATGAGGAAGAAGAGGAGGA AGATGATGATAGGCCCCCCAAGAAACCACGCCATGGGGGCTTCATTTTAGATGAGGCTGATGTAGATGAT GAATATGAAGATGAAGACCAGTGGGAAGATGGAGCTGAAGATATCCTGGAGAAAGAAGAGATTGAAGCCT CCAATATTGATAACGTTGTGCTGGATGAAGACCGCTCTGGGGCTCGTCGTCTGCAGAATCTCTGGAGGGA CCAGAGAGAAGAAGAACTGGGCGAGTATTACATGAAGAAATATGCTAAGTCGTCTGTGGGAGAGACGGTG TATGGAGGGTCTGACGAGCTCTCAGATGACATCACTCAGCAGCAGCTGCTCCCAGGAGTCAAGGACCCCA ACCTGTGGACTGTCAAGTGTAAGATTGGGGAGGAACGAGCCACGGCAATTTCCTTGATGCGGAAATTCAT CGCTTACCAGTTCACAGACACACCCCTGCAGATTAAGTCAGTGGTTGCCCCTGAGCACGTGAAGGGCTAC ATCTATGTGGAGGCCTACAAACAGACCCATGTGAAGCAGGCCATTGAAGGTGTGGGCAACCTGCGGCTTG GCTACTGGAACCAGCAGATGGTGCCTATTAAGGAGATGACCGATGTACTCAAGGTGGTGAAGGAGGTGGC CAACCTGAAGCCGAAGTCCTGGGTCCGCCTCAAGCGAGGCATCTACAAAGATGACATTGCTCAGGTGGAC TATGTGGAGCCCAGCCAGAACACCATTTCCCTGAAGATGATCCCGCGCATCGACTACGACCGCATCAAAG CCCGAATGAGCCTGAAAGACTGGTTTGCCAAAAGGAAGAAGTTCAAGCGGCCTCCACAGAGGCTCTTTGA TGCAGAGAAGATCAGGTCCTTGGGGGGTGATGTTGCCTCTGATGGTGACTTCCTCATCTTTGAGGGGAAC CGCTACAGCCGAAAGGGCTTCCTGTTCAAGAGCTTTGCCATGTCTGCTGTGATTACAGAAGGTGTGAAGC CCACACTGTCAGAGCTGGAAAAGTTTGAAGATCAGCCTGAGGGCATCGACCTGGAGGTTGTGACTGAAAG CACAGGGAAGGAGCGGGAACACAATTTCCAGCCTGGGGACAATGTGGAGGTGTGCGAGGGCGAGCTCATC AACCTACAGGGCAAGGTCCTCAGCGTGGATGGCAACAAGATCACTATCATGCCCAAGCACGAGGACCTCA AGGACATGCTTGAGTTCCCCGCTCAGGAACTTCGGAAGTACTTCAAGATGGGGGACCACGTAAAAGTGAT TGCTGGCCGATTTGAGGGTGACACAGGTCTCATTGTGCGGGTGGAAGAGAACTTTGTTATTCTCTTTTCA GACCTCACCATGCATGAGTTGAAAGTGCTTCCCCGAGACCTGCAGCTCTGCTCAGAGACAGCCTCAGGCG TGGATGTTGGGGGCCAGCATGAGTGGGGTGAGCTGGTGCAGCTGGATCCCCGTACTGTGGGTGTCATCGT GCGGCTAGAGCGAGAAACCTTCCAGGTTCTCAACATGCACGGGAAGGTGGTGACCGTTAGGCACCAGGCC GTGACGCAGAAGAAGGACAACCGCTTCGCTGTGGCTCTGGACTCGGATCAGAACAACATCCATGTGAAGG ACATTGTCAAGGTCATCGATGGCCCTCACTCAGGCCGAGAGGGTGAGATCCGCCATCTGTACCGCAGCTT TGCCTTCCTGCACTGCAAGAAGCTGGTGGAGAACGGCGGCATGTTTGTCTGTAAGGCACGGCACCTGGTG TTGGCTGGGGGCTCAAAGCCCCGGGATGTGACCAATTTAACTGTTGGTGGCTTCACTCCTATGAGCCCCC GGATCAGCAGCCCTATGCACCCCAGTGCTGAAGGTCAGCATGGTGGCTTTGGAAGCCCAGGTGGCATGAG CAGGGGTCGAGGGAGAAGAGACAATGAACTCATAGGCCAGACTGTGCGTATCTCCCAGGGACCCTACAAA GGCTACATTGGTGTGGTGAAGGATGCCACAGAGTCCACGGCCAGAGTAGAACTGCATTCTACCTGCCAGA CCATCTCTGTGGATCGCCAGCGGCTCACCACGGTCGACTCCCAGCGTCCAGGTGGCATGACCTCTACATA TGGACGGACTCCCATGTATGGCTCTCAGACACCCATGTATGGCTCTGGCTCCCGAACACCTATGTATGGC TCCCAGACACCTTTGCAGGATGGCAGCCGCACTCCACATTATGGCTCACAGACACCCCTGCATGATGGTA GCCGCACTCCTGCTCAGAGTGGGGCCTGGGACCCCAACAACCCAAACACGCCTTCCAGGGCTGAGGAGGA ATACGAATATGCCTTTGACGACGAACCCACCCCATCCCCACAAGCATATGGGGGAACCCCGAATCCCCAA ACACCCGGCTACCCAGACCCATCATCCCCCCAGGTCAATCCACAGTACAATCCACAGACACCAGGGACGC CAGCCATGTACAACACAGACCAGTTCTCCCCCTACGCTGCCCCCTCTCCACAGGGGTCTTACCAGCCCAG CCCCAGCCCCCAGAGCTACCACCAAGTGGCACCAAGTCCAGCAGGCTATCAGAACACCCATTCCCCAGCC AGCTATCACCCGACACCGTCACCTATGGCCTATCAGGCTAGTCCCAGCCCAAGCCCTGTTGGCTACAGTC CAATGACCCCTGGAGCACCTTCTCCTGGAGGTTACAATCCCCACACACCGGGCTCAGGCATTGAGCAGAA CTCTAGCGACTGGGTGACCACTGATATCCAGGTGAAGGTTCGAGACACCTATCTGGACACACAAATAGTG GGGCAGACAGGCGTCATCCGCAGTGTCACGGGAGGCATGTGCTCCGTGTACCTGAAGGACAGTGAGAAGG TGGTCAGCATCTCCAGTGAACATCTGGAGCCCATCACGCCCACCAAGAACAACAAGGTGAAGGTGATTCT GGGTGAGGACCGAGAGGCTACCGGGGTCCTACTGAGCATTGATGGTGAGGATGGCATCATCCGCATGGAC CTAGAGGACCAGCAGATCAAGATTCTAAACCTCCGCTTCCTAGGGAAGCTCCTGGAAGCCTGACGCCCAA GTGTGCAGTGCAGACATGGAGCCAGCAGCCCCTCTGGCCTTGTTTGGCCTAGCATGGAACCCTGAGTCTG GAGCAAGCCAGGGCAATCACTCTGTATTTCCTTTTCCTCCTTCCCTGATACTCTAGGAGTCAGAGCAGAG TCTGAGGAATGTTCCTGCCTTCTTCTGCAGCCCCAGCTTGACTTTGTGTTCAGGTCTTTCAATAAAGAGA AGCTGTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3496691 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496691 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004056 same experiment
	EMAGE:30945 same embryo
	EMAGE:30025 same embryo
	EMAGE:30012 same embryo
	EMAGE:29973 same embryo
	EMAGE:30028 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source