EMAGE mouse gene expression spatial database

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EMAGE:30901

D16Ertd472e ( MGI:1196400)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005119_01	euxassay_005119_02	euxassay_005119_03	euxassay_005119_04	euxassay_005119_05

euxassay_005119_06	euxassay_005119_07	euxassay_005119_08	euxassay_005119_09	euxassay_005119_10

euxassay_005119_11	euxassay_005119_12	euxassay_005119_13	euxassay_005119_14	euxassay_005119_15

euxassay_005119_16	euxassay_005119_17	euxassay_005119_18	euxassay_005119_19	euxassay_005119_20

euxassay_005119_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cranial musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

foot

strong

regional

strong expression: see section 05 06 16 17

lower jaw molar

strong

regional

strong expression: see section 16 moderate expression: see section 06

upper jaw molar

strong

regional

strong expression: see section 16 moderate expression: see section 06

thymus primordium

moderate

regional

moderate expression: see section 07 08 09 10 11

vibrissa

strong

regional

strong expression: see section 05 06 07 08 16 17 18 19 20 moderate expression: see section 21

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 08 09 10 11 12 13

upper lip

strong

regional

strong expression: see section 09 10 11 12 13 14

midbrain marginal layer

moderate

regional

moderate expression: see section 07

lower jaw incisor

moderate

regional

moderate expression: see section 09 10 12 13

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

lower lip

strong

regional

strong expression: see section 08 09 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6875
Entity Detected:	D16Ertd472e, ( MGI:1196400)
Sequence:	sense strand is shown >T6875 CGGATCGTGGCGCGCGGACGGTGGCCCGCGGAGCCGGACGGTAGAGGTCCTTCTCTGAGCCGCTCTGGTG CTCAGGACCAGTGCCGGGACGGACGGACAAACGAGACGGATCCCACGCCGTGCCGGACGCCCACGATCCT CGAGTGGCGTCCCCGGGACCCTCCGGTCCGTGGCTGAAGAGTCTCCGACGGGCACTATGAACGAAGAGGA GCAGTTTGTAAGCATTGACTTGAATGATGACAACATTTGCAGTGTTTGTAAACTGGGAACAGACAAAGAC ACACTCTCCTTCTGCCACATTTGCTTCGAGCTAAATCTCGAGGGAGTGCCAAAGTCGAATCTTTTACACA CCAAATCAGTGAGAGGCCATAAAGACTGCTTTGAAAAATACCATTTAATAGCAAACCAGGATTGTTCTCG TTCCAAGCTTTCCAAAAGTACTTATGAAGGAGTTAAAACCATCGTGAGTAAGAAGATAAACTGGATTGTG CAGTATGCCCAAAATAAAAATCTGGATTTGGAGTCCGAGTGTTCCAAAACTTCCCAGCATCCCCTGTTGA ATTTCAGGCATAAGCCAGAGAAGAAATTGCTCCCACAGTTTGACTCTCAGGTGCCAAAGTACTCGGCAAA AGGCTCAGCCGGAAACGCAGGCAGCATCTCAAGCTATGCGCAGAGAATCCTGGAGCACAGAGAAAATACA GACTTCGGGCTGGGTTTATTGGAAGACGCGGATGCATTGTGGACTCACAGTCACAGCCAGGCACAGAAAA CAGAAGAGACAAGCTCTGGTCCGGAGGGGACTATCCAGACCCAGAATCCACATTACAGCCGTGAAGAATT GAATTCGATGACTCTTGCTGAAGTAGTACAACTGAGTGCGAAGCTCCAACAGCGAATCCAAGAGGTTTTT GAAGAGTTAACACACCAGGTGCAAGAGAAGGACTCTCTGGCGTCAGAGCTCCATGTCCGTCACGTGGCTA TCGAGCAACTCCTCAAGAACTGCTCCAAGTTGCCATGCCTCCAAGTGGGACGGACAGGGACGAGGTCACA CCTACCCATGAACCACTGAAGGGACCGGAGCTGTCCTGCTTTCATCCTCTACTGCTCTGCTAAGCATATG GACCGAGAAAACATGGGAAGGAAGTCATGGTCCATTTTTATGGTTGTTGTGCTTGCAAAACCTAAGATAA TACTCATTATCTTTATCTAATAAAGCAGATCACTCTAGTCCAGGGTTACTCAATCATTTGGCTTCATCGG GGGACTTCTTATAATTACTAAATGCCCCTCCCTATCTTTTACCATATGTGACTACTTAAATATTCTGTTA TAGTGTTTTTACTGACCAATTTTTATTGTAACTCAGTTGTCAAAAACAATAGAAGCCTAGTAAGGTCTTA TGTTCACCTAAACTACTAGAGAGAATTTTTAAAAGGGAAATAGAATTCGTAATATCACCTTATTTATGAG CAATATTTTAATATAAAATTTTATATAGAGAATTGCTATTTTGGGTATTTTTCATTGTCTTTATAAGTCT GTATTTGAATGGCCATGTGTATTTGCACTTCCTGTGTGAATATGTTCACCTCTGTCAAGATCGCTGCATT TTGGCAATACGGTGTTTTTATAACTTATCTTTCTTTCCAAAGATACATGTGTTTCGGATAGTATTCGGAG AAGCAGTCTACCTAGCTGAGCTGTTTTAACTACTTAACAGAATATGAAAGTGGAATTAATCGTTCCTTTA AAGCAAATATCCAAGTAAACAAAGCAGCAGATTATTGTTTTCTAATGGAAACAGTGCTGAATTCGCTTAG GAAGAAAAGACTATTCTAGTTTGTATGTGTCAGTCTCCATAAAAGTAGTGGCCCTGCCCTGCTTCTTAAT TCTCAACACTTTCTTGGATGTGTTAGTTTTGGACAAAGTAGGTCAACTATCTTCAGACTGCACTGGTCTT CCTTTGGGGGAAAAAATAAAATTATTATGTGTGCCACTTCACATGGAGTTCCCGTAATCTACGAACTGTC CAAATTCTGCTATCCACATCACTGAAATTAGTCCAAAAAATAATAATAACAAACCATTAAAGAAAGAAAA GGAAAAGCATTTCAAGGTACTTTTCTAAGTTTTAAAACTCTAAAGGGATAGACGATTACAGCACCACCAC CCCCCAAAAAAACTTCAATAAAAGGATAGTATAAGCTACTTTGGTTTCATATTCTTAGCTACTAAGTTAA TGATGACACAAAAGCTAGTGCAAAATTAAGATGAACTCTTTAGCTGATGCTTAGAATGTATTTGTGTGGC TGATAGGGTTTGCCTGCAGAAATCTAAGGAAAGAAAACCCATTCATTGACTTGGCAGCCCCTCACTTGGC AGCCCCTCATGCATAAAGATGGTTTCTGCCTTGAAGTCGGCAATCTGTATCTGGGCATGGGTCAGACTTT AATATTGAACATAAATAATATTTGACTAAGCAAACAGCTGCCTCACTGTAGAAAAAGCACCTTTTCCTGA TGCTAGGATGTGTTAAACTGGCCTGGCTGTGTGCATTAGTCAATGTCCCATTGGTTTTGCTCTGTCCTGA AGAAAACTTACCTTTGAAGCTTTTTGGAAGGAGTTTTTGGGGAAAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3966397 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3966397 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005119 same experiment
	EMAGE:31606 same embryo
	EMAGE:31615 same embryo
	EMAGE:31184 same embryo
	EMAGE:31026 same embryo
	EMAGE:30216 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source