EMAGE mouse gene expression spatial database

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EMAGE:30911

Csrp3 cysteine and glycine-rich protein 3 ( MGI:1330824)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010953_01	euxassay_010953_02	euxassay_010953_03	euxassay_010953_04	euxassay_010953_05

euxassay_010953_06	euxassay_010953_07	euxassay_010953_08	euxassay_010953_09	euxassay_010953_10

euxassay_010953_11	euxassay_010953_12	euxassay_010953_13	euxassay_010953_14	euxassay_010953_15

euxassay_010953_16	euxassay_010953_17	euxassay_010953_18	euxassay_010953_19	euxassay_010953_20

euxassay_010953_21	euxassay_010953_22	euxassay_010953_23	euxassay_010953_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20

tongue muscle

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

foot

moderate

regional

moderate expression: see section 05 06 07 08 09 22 23 24

eye skeletal muscle

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 18 19 20 21 22 23 24

heart atrium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

leg muscle

strong

regional

strong expression: see section 07 09 10 11 21 22 23 moderate expression: see section 01 02 03 04 05 06 08 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 06

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06

hand

moderate

regional

moderate expression: see section 01 02 03 04 05

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36210
Entity Detected:	Csrp3, cysteine and glycine-rich protein 3 ( MGI:1330824)
Sequence:	sense strand is shown >T36210 AGAGTCTTCACCATGCCAAACTGGGGTGGAGGTGCAAAATGTGGAGCCTGTGAAAAGACGGTCTACCATG CAGAAGAAATCCAGTGCAATGGGAGGAGTTTCCACAAGACCTGTTTCCACTGCATGGCCTGCAGGAAAGC TCTGGACAGCACCACAGTGGCAGCTCATGAGTCAGAGATCTACTGTAAGGTGTGCTATGGGCGCAGGTAT GGCCCCAAGGGGATCGGGTTCGGACAAGGCGCTGGCTGCCTCAGCACAGACACTGGCGAGCATCTTGGCC TGCAGTTCCAACAATCCCCAAAGCCAGCTCGAGCAGCCACCACAAGCAACCCTTCCAAATTCTCTGCAAA GTTTGGAGAATCAGAGAAGTGCCCACGATGTGGAAAGTCGGTATACGCTGCTGAGAAGGTCATGGGAGGT GGCAAGCCCTGGCACAAGACCTGCTTCCGCTGTGCCATCTGTGGGAAGAGCCTGGAGTCTACAAATGTCA CTGACAAGGATGGGGAGCTCTACTGCAAAGTTTGCTATGCCAAAAATTTTGGCCCCACAGGCATTGGGTT TGGAGGGCTTACACAGCAAGTGGAAAAGAAGGAGTGAAGCTGCCTGCTTTCTCACAGCCCTATCAGTCAA CAGCAGTGCCACGTGATCCCACAGATGTAGTTTGTCCCCAACAGCCTCCTTTGTAGCTGGACAGCATTTC TCTTCAGAAGCAGTCATAGGCTGCACCGAAGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96599. Forward Primer - name:096599_F_cDNA_Csrp3, sequence:AGAGTCTTCACCATGCCAAACT; Reverse Primer - name:096599_N_SP6_cDNA_Csrp3, sequence:ACTTCGGTGCAGCCTATGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010953 same experiment
	EMAGE:30913 same embryo
	EMAGE:30912 same embryo
	EMAGE:31143 same embryo
	EMAGE:31086 same embryo
	EMAGE:29359 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source