EMAGE mouse gene expression spatial database

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EMAGE:30915

Abce1 ( MGI:1195458)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006203_01	euxassay_006203_02	euxassay_006203_03	euxassay_006203_04	euxassay_006203_05

euxassay_006203_06	euxassay_006203_07	euxassay_006203_08	euxassay_006203_09	euxassay_006203_10

euxassay_006203_11	euxassay_006203_12	euxassay_006203_13	euxassay_006203_14	euxassay_006203_15

euxassay_006203_16	euxassay_006203_17	euxassay_006203_18	euxassay_006203_19	euxassay_006203_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 14 weak expression: see section 07 13

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20

thymus primordium

moderate

regional

moderate expression: see section 07 08 09 10 11

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 16 17

frontal bone primordium

moderate

regional

moderate expression: see section 01 02 03 04

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 weak expression: see section 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T615
Entity Detected:	Abce1, ( MGI:1195458)
Sequence:	sense strand is shown >T615 GTTGGCCTACTGGGTTTAAGGCTGCCATTACGATTCGATCTCTAATAAATCCAGATAGATATATCATTGT GGTGGAGCATGATCTAAGTGTATTAGACTATCTCTCTGACTTCATCTGCTGTCTATATGGGGTACCGAGT GCTTATGGTGTTGTCACGATGCCTTTTAGTGTAAGAGAAGGCATAAATATATTTTTGGATGGCTATGTTC CAACAGAGAACCTGAGGTTCAGGGATGCGTCGCTTGTTTTTAAGGTAGCTGAGACAGCAAATGAAGAAGA AGTTAAAAAGATGTGCATGTATAAATATCCCGGGATGAAGAAAAAGATGGGAGAGTTCGAGCTAGCAATT GTAGCTGGAGAGTTCACGGACTCTGAGATCATGGTGATGCTGGGGGAGAATGGTACAGGTAAAACTACAT TTATCAGAATGCTTGCTGGAAGGCTTAAACCAGATGAAGGAGGAGAAGTGCCAGTTCTAAATGTCAGTTA TAAGCCACAGAAAATCAGTCCCAAATCAACAGGAAGTGTTCGCCAGTTACTGCATGAAAAGA
Notes:	The probe template was PCR amplified from IMAGE:1886091 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1886091 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006203 same experiment
	EMAGE:31791 same embryo
	EMAGE:30805 same embryo
	EMAGE:31810 same embryo
	EMAGE:31776 same embryo
	EMAGE:30874 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source