EMAGE mouse gene expression spatial database

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EMAGE:30966

Mfng MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ( MGI:1095404)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004928_01	euxassay_004928_02	euxassay_004928_03	euxassay_004928_04	euxassay_004928_05

euxassay_004928_06	euxassay_004928_07	euxassay_004928_08	euxassay_004928_09	euxassay_004928_10

euxassay_004928_11	euxassay_004928_12	euxassay_004928_13	euxassay_004928_14	euxassay_004928_15

euxassay_004928_16	euxassay_004928_17	euxassay_004928_18	euxassay_004928_19	euxassay_004928_20

euxassay_004928_21	euxassay_004928_22	euxassay_004928_23	euxassay_004928_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 22 23 24

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 12 16 17

pituitary gland

strong

regional

strong expression: see section 09 10 11 12 13 14

stomach

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09

metanephros

weak

regional

weak expression: see section 04 05 06 07 08 14 15 16 17

midgut

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19

bladder

weak

regional

weak expression: see section 12 13 14

esophagus

weak

regional

weak expression: see section 09 10

vomeronasal organ

strong

regional

strong expression: see section 12 14 15

thymus primordium

weak

regional

weak expression: see section 08 09 10 11 12

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 18 19

hindgut

weak

regional

weak expression: see section 13 14

hypothalamus ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14 15

lung

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6922
Entity Detected:	Mfng, MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ( MGI:1095404)
Sequence:	sense strand is shown >T6922 CCACGCGTCCGGCCGGCTGGCAGGACGGTGGTGGCTCAGTCTCTGGGTCCAGAGACCCAGCTTCCTCCTT TCCCGTCGGTGTGTGCCCCCGATGCCTGCCAGACTTTTGCCTGTACCAGGGCCCCCTCCTGACCAGCCTC CCACCCCAGACTGATTGGGGAGTCGGGGGCTGAACTGTCTGGGGGCCTGCTACCAATGCACTGCCGACTT TTTCGGGGCATGGCGGGAGCCCTCTTTACCCTCCTGTGCGTGGGGCTCCTGTCTCTACGATACCACTCAA GTTTGTCCCAGAGGATGATACAGGGCGCGCTCAGGCTGAACCAACGGAACCCAGGACCCCTGGAGCTGCA GCTAGGCGACATCTTCATCGCAGTCAAGACTACCTGGGCCTTCCATCGCTCCCGCCTGGACCTGCTACTA GACACGTGGGTCTCCAGGATCAGGCAACAGACATTCATCTTCACTGACAGCCCAGATGAACGCCTCCAGG AGAGACTAGGCCCGCACCTCGTGGTCACCAACTGTTCTGCAGAGCACAGTCATCCTGCTCTGTCCTGCAA GATGGCTGCAGAGTTCGATGCCTTCTTGGTCAGTGGCCTCAGGTGGTTCTGCCACGTGGATGATGACAAC TATGTGAACCCCAAGGCTCTGCTGCAGCTGTTGAAAACATTCCCGCAGGACCGTGATGTCTATGTGGGCA AGCCCAGCCTGAACCGGCCCATCCACGCCTCTGAGCTGCAGTCAAAAAACCGCACGAAGCTGGTGCGGTT CTGGTTTGCCACAGGGGGTGCTGGTTTCTGCATCAACCGCCAACTGGCTTTGAAGATGGTGCCATGGGCC AGCGGCTCCCACTTTGTGGACACTTCTGCTCTCATCCGGCTCCCCGATGACTGCACTGTGGGCTACATCA TCGAGTGCAAGCTGGGGGGTCGCCTGCAGCCCAGCCCCCTCTTCCACTCACACCTGGAAACCCTGCAGCT GCTGGGGGCTGCCCAGCTTCCGGAGCAGGTCACCCTCAGCTACGGTGTCTTTGAGGGGAAACTGAATGTC ATCAAGCTACCGGGCCCCTTCTCCCATGAAGAGGACCCCTCCAGATTCCGCTCCCTCCATTGTCTCCTCT ACCCAGACACACCCTGGTGTCCGCTGCTGGCAGCGCCCTGAGCTCTGAACTGCCGCAAAGGATGATGGAT GCTACTGCCTGACCACTCCCTCACCTCCCAAGCAGTCCTGAGGGCTCTCAGTCCAGTGCACACTGGCAGA TGGTCCCTGGCAGGGCCTCTGGGTGACAGGAAAGGCTACGATATGGACACTGCTTGGTGTTGAGGGTGTC TTGGGCTGGTCATACCACAGAGGCGGCAGGAACGCTTGAGTTGGTGTGTGGGAGGGGATAGGGGTCCTCA CCCTCCTGAGGGTGACATGTGCCCTGCATGGAATCTAGCCTCTGGCTTTGGCCTCAGTCATTCCTTAGAG ACTAGAGTCATTGGGAGAAGGCCAGGGTCTGTGACCTGCGGCTGTGGAACTGGTCTCAGCTGCTCCTGGC GCTGCTCAGCTGGCCAATGGACCTAGACTTTAGAAGCCCAGAAGGCTCAGGTGCCAATTGCAACCGACCT TTGGTCACTGTCCCAGCCTTCTCTGCTGTGAGGAGCTGCCATGTGACCCAGTGGAACTGAGCAAAGCCAA TAGAAGGGTTTCTGGAATACATTCCCAGCCCAAGACCCCATCTTCCTACTATGAATGTGGGTGTGACGGC TGAATCTGGGGAAACTACTTTGGCACCATGAAGGAAAGGCCAAGACAATCTAAAGTTGTTCTCCCTAACA CCTCATCTGTAAGAAATTAAACCTTTATTTAAGTCTCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3980692 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3980692 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004928 same experiment
	EMAGE:30083 same embryo
	EMAGE:30201 same embryo
	EMAGE:30128 same embryo
	EMAGE:30951 same embryo
	EMAGE:30984 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source