EMAGE mouse gene expression spatial database

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EMAGE:30984

S100a16 ( MGI:1915110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004932_01	euxassay_004932_02	euxassay_004932_03	euxassay_004932_04	euxassay_004932_05

euxassay_004932_06	euxassay_004932_07	euxassay_004932_08	euxassay_004932_09	euxassay_004932_10

euxassay_004932_11	euxassay_004932_12	euxassay_004932_13	euxassay_004932_14	euxassay_004932_15

euxassay_004932_16	euxassay_004932_17	euxassay_004932_18	euxassay_004932_19	euxassay_004932_20

euxassay_004932_21	euxassay_004932_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

weak

regional

weak expression: see section 10 11 16 17

stomach

strong

regional

strong expression: see section 01 02 05 moderate expression: see section 03 04 06

metanephros

moderate

regional

moderate expression: see section 15 16 weak expression: see section 04 05 06 07 13 14

rectum

strong

regional

strong expression: see section 12 13 14

urethra of male

strong

regional

strong expression: see section 12 13 14 moderate expression: see section 15

midgut

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18

bladder

strong

regional

strong expression: see section 11 12 13

esophagus

strong

regional

strong expression: see section 08 09 moderate expression: see section 10

submandibular gland primordium

moderate

regional

moderate expression: see section 04 05 16 weak expression: see section 06 07 14 15

thymus primordium

weak

regional

weak expression: see section 07 08 09 10 11

vibrissa

strong

regional

strong expression: see section 03 04 05 06 07 19 20 21

telencephalon ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 10 15 16 17

hindgut

weak

regional

weak expression: see section 11 12 13 14 15

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

naris

strong

regional

strong expression: see section 11 12 moderate expression: see section 14 15

adrenal medulla

weak

regional

weak expression: see section 04 05 06 07 13 14

lung

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6920
Entity Detected:	S100a16, ( MGI:1915110)
Sequence:	sense strand is shown >T6920 GGGTGATGCGTGTAGCCTGTGCCAGGCGGCAACTGTGTCCAGAGGCCCAGAGTCTGACTCCAGCTGGGGC AGAACTGGGCCAGGATCTTCCTTAGGCCCAGAGAGGAGCTGAGGCAGTGAGATGGCTGACTGCTATACAG AGCTGGAGAAGGCAGTTGTTGTCCTGGTGGAAAACTTTTATAAATATGTATCCAAGCACAGCCTGGTCAA AAACAAGATCAGCAAGTCCAGCTTCCGAAAGATGCTCCAGAGAGAGTTGAACCACATGCTGACGGACACA GGGAACCGAAAGGCAGCTGACAAGCTCATCCAGAACCTGGATGCCAACCACGACGGGCGCATCTGCTTTG ACGAATACTGGACCATGATTGGTGGAATCACCAGCCCCATGGCCAACCTCATCCGCCAACAGGAGTGCCA GCAGGAGAGCCAGCAGGAGTGCCAGCAGGAGAGCCAGCAGGAGAGCCAGCAGGAGAGCCAGCAGGGCAGC AGCTAGAGGCCCCTCGTGGGCCCCTTTTCATGTCCTGACCCATGTCCTGCCAGTGTTCTTTCCATGCTTT AAGTTGACTCTTTCTTCCTCCCTTCTCCCTCCAGACCTGTTCCTACCCTTGCTGAACAGCACAAGATGGG TGGGTCGGGGGCTATCAAGGCCACTGTGGTAGTGCTTCCCTGAGCATTCAATCCCTGGATCCTGTAGGCC CTGGGAGGGGGTCCCCTTTGTGAAATATCAATGCTGTTTGGGGCCCTCCCCCCAAGACTTCTCTCACTTG TTGAGTCTGTGTGCTCTCGCAATGTTCCTGTCTAATTCTTGACTGATTCTGGGTCTGTCCTGACCTCTAG AGGTGAGCTTGCTGCTTGAGGTCTCCCAGCTCCTGACTGTGGCAGCTGTGGTAGTGGCAGTACCAGAATT AGCCGCTTCTTTTCCTCCCAACCTCTTAGCCGGGTAGAGAGGCATTCCCAGGGGCTCCTGTCCAGCTGTC TCTGTCTGGGAATGATCTAACCTGGGTTGAGCCTCCTCTCTGCTCTGCCACCGAAATAAACGTTGAATTT GGAGTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3980274 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3980274 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004932 same experiment
	EMAGE:30083 same embryo
	EMAGE:30966 same embryo
	EMAGE:30201 same embryo
	EMAGE:30128 same embryo
	EMAGE:30951 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source