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EMAGE:31010

Dab1 disabled homolog 1 (Drosophila) ( MGI:108554)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31010 EMAGE:31010 EMAGE:31010 EMAGE:31010 EMAGE:31010
euxassay_001879_01 euxassay_001879_02 euxassay_001879_03 euxassay_001879_04 euxassay_001879_05
EMAGE:31010 EMAGE:31010 EMAGE:31010 EMAGE:31010 EMAGE:31010
euxassay_001879_06 euxassay_001879_07 euxassay_001879_08 euxassay_001879_09 euxassay_001879_10
EMAGE:31010 EMAGE:31010 EMAGE:31010 EMAGE:31010 EMAGE:31010
euxassay_001879_11 euxassay_001879_12 euxassay_001879_13 euxassay_001879_14 euxassay_001879_15
EMAGE:31010 EMAGE:31010 EMAGE:31010 EMAGE:31010 EMAGE:31010
euxassay_001879_16 euxassay_001879_17 euxassay_001879_18 euxassay_001879_19 euxassay_001879_20
EMAGE:31010 EMAGE:31010 EMAGE:31010
euxassay_001879_21 euxassay_001879_22 euxassay_001879_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
strong strong
homogeneousstrong expression: see section 10 11 14 15 16
ventral grey horn
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 07 09 11 12 13 14 15 16
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 13 14 15 16 17 18 moderate expression: see section 09 11 12
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15
telencephalon ventricular layer
strong strong
homogeneousstrong expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20
upper lip
moderate moderate
regionalmoderate expression: see section 06 07 08 09
telencephalon marginal layer
strong strong
homogeneousstrong expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 16 17 18 19 20 21 22
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15
lower lip
moderate moderate
regionalmoderate expression: see section 06 07
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1141
Entity Detected:Dab1, disabled homolog 1 (Drosophila) ( MGI:108554)
Sequence:sense strand is shown

>T1141
CCTCGAGCCTGTTGGCCTACTGGATCACCAGTCAAGCCAACGCTTAACCAACAAATACCTTGAGCTCTCT
CAGCTGGGTCTAAGACGCCCCTGGATTTGAAACCGTTACTGTGTTTTGCTTTTGCTACCTGTTGACCACT
GTAGAGACCACAGATTGGGAGTGGCAGGCATCAGAGAATTGTGACAAGGTTGGCACTGACTCGTTTGAGG
CCAAAGCAAGGGATCCTTTGTGACCACAGTGTCATCCGTATGCCTTCTGGCTGTGGCGACGTGCGTGCGC
GCGTGCGTGGCATCTCTCCACGGGGCCTGGTCTGCCATCCTGTCTCCCCTGTCCTTTCTTTGTCCTGTTT
GAGAATTAATGGGTGTACAAATTTTTGTATTGCTTTTGACTTTTTTAGAAATGGGTGAAGAAGTCTAAAT
GGGGGAGGGAAAGCCTCTTATGAAGTGAATTAACTTGAATCACAAGGCAATGACGTGGTTGGTGATTCAT
TATGATCAAGAATGTTGCCAAAACAACTCTGTAGTTGTCCTGTGTCATGGTGAAGAAAGATGGCTTTTGG
CTAGAACCTTCATTC
Notes:The probe template was PCR amplified from IMAGE:2135921 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2135921 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001879 same experiment
 EMAGE:31802 same embryo
 EMAGE:30575 same embryo
 EMAGE:31794 same embryo
 EMAGE:29315 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS