EMAGE mouse gene expression spatial database

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EMAGE:31020

Cckar ( MGI:99478)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011441_01	euxassay_011441_02	euxassay_011441_03	euxassay_011441_04	euxassay_011441_05

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euxassay_011441_11	euxassay_011441_12	euxassay_011441_13	euxassay_011441_14	euxassay_011441_15

euxassay_011441_16	euxassay_011441_17	euxassay_011441_18	euxassay_011441_19	euxassay_011441_20

euxassay_011441_21	euxassay_011441_22	euxassay_011441_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

trachea

moderate

regional

moderate expression: see section 13 14 15

cerebral cortex mantle layer

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23

lung

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

thyroid gland

moderate

regional

moderate expression: see section 12 13 16 weak expression: see section 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30132
Entity Detected:	Cckar, ( MGI:99478)
Sequence:	sense strand is shown >T30132 AACAAACGCTTTCGCCTGGGCTTCATGGCCACCTTCCCCTGTTGCCCGAATCCTGGTCCCACCGGGGTGA GAGGAGAAGTGGGAGAGGAGGAAGATGGAAGGACCATAAGGGCATCCCTGTCCCGGTATTCCTACAGCCA CATGAGCACCTCTGCCCCACCCCCCTGAGCTGTACCTGGTTCGCTGCGGGCAGCAGAAAGGAGGGGACTG GGAGCAAGGAGGAAGAGGAAGTGGGAGGGAAGGAGAAGGAAGACCCATTTCTCACGAGGACTCTTCCGAT GTCTGCTTTTCACCCTTCACAAAGTTCCAGAAGGAACTTAAGGCGGGGTCATGAAAACGCTAAGACATTT TCCATAATGTTTACCATGGGAGAAACCTCAGCAGGCCAGCGATAGGGACCCCACTGGGCTCGTGCTACAA ATGTCACTGCCAAGTCCACGTTCAAAATGGGGGTGTAGACCTTGATGAGCGTTAGAATTTTTTGGAACGC CCCAGATGACGTCTCTCCATACTAACACACTGCTCTGCTCTGTTTTCTTCTGCTCCTGAAGAATTCTGGT CCTCCCCATGCCTACTTCTCACACATACTACAGCTACACACCTTCTCATGGCCACCAATGACATGTGACC ACGCACGCGCACACACCTCCTCTGGCCTCTTCTGAATAAGGGAGAAGTCTGACAAATTTTAAAAGCTTGA GGTTGTACAAGTATACTCAGGGGTGAAAGGTTGAGGTTGTCATCAGAAATACATGAAGAATGTGTCTAAA AGACAGGTGTTAGGATTTTTATTTCTACAAAGGACAGGGTATGCGAGGCCAGTAGCTAGAACTGTTTTCT GTCAGCAATGTCAAGCTGCAGAAGGATGATCTCTACAGTATAATCTCTCCTCTGAAGCTAATCCCCACAA CCTTTTTTTTTCTTTAACCTCAACTCACTTTCTTCTGTTCACCTATATTTCCTCCTTTCCAGAAATGCCT CCGGGTAAGAAGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4236240), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 9259. Forward Primer - name:ABA_MGC_046_02_H11_F_TGGACCCCAGCCATAGAA_ABA_FR_046, sequence:AACAAACGCTTTCGCCTG; Reverse Primer - name:_R_SP6_ABA_MGC_006_02_E11_AACAAACGCTTTCGCCTGGGCTTC, sequence:TCCCTTCTTACCCGGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011441 same experiment
	EMAGE:29337 same embryo
	EMAGE:29326 same embryo
	EMAGE:30996 same embryo
	EMAGE:31027 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source