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EMAGE:31021

Tmem132d ( MGI:3044963)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31021 EMAGE:31021 EMAGE:31021 EMAGE:31021 EMAGE:31021
euxassay_010351_01 euxassay_010351_02 euxassay_010351_03 euxassay_010351_04 euxassay_010351_05
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euxassay_010351_06 euxassay_010351_07 euxassay_010351_08 euxassay_010351_09 euxassay_010351_10
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euxassay_010351_11 euxassay_010351_12 euxassay_010351_13 euxassay_010351_14 euxassay_010351_15
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euxassay_010351_21 euxassay_010351_22 euxassay_010351_23 euxassay_010351_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
mandible
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 11 12 16 17 19 20 21 22
radius
weak weak
regionalweak expression: see section 01
olfactory cortex marginal layer
weak weak
regionalweak expression: see section 12 13 16 17 18
humerus
weak weak
regionalweak expression: see section 02 03 04 20 21 22 23 24
fibula
weak weak
regionalweak expression: see section 01 22 24
pelvic girdle skeleton
weak weak
regionalweak expression: see section 16
rib
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21
rest of cerebellum mantle layer
weak weak
regionalweak expression: see section 02 03 04 05 06 07 12 13 14 15 16 17 18 19
axial skeleton
weak weak
regionalweak expression: see section 07 08 09 10 11 12 13 14 15
tibia
weak weak
regionalweak expression: see section 01 22 24
scapula
weak weak
regionalweak expression: see section 20 21
femur
moderate moderate
regionalmoderate expression: see section 05 20 21 weak expression: see section 03 04 06 07 08 09 19 22 23
maxilla
weak weak
regionalweak expression: see section 06 07 08 09 11 12 16 19 20 21 22
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37612
Entity Detected:Tmem132d, ( MGI:3044963)
Sequence:sense strand is shown

>T37612
GACATCACGGAGCTGATAACTGACTTCATGCAGGTAGAGGAGCCCAGGATTGCTAAGCTGCAAGGAGGGC
AGATCCTGACTGGCCAGGAGCTGGGCATGACCACTATTCAGATCCTGTCCCCTCTGTCGGATGCCATCCT
TGCCGAGAAGACCATCACTGTACTGGATGAGAAGGTGACCATCACAGACCTCGGGGTCCAGCTGGTAACA
GGGCTCTCGCTATCCCTGCAGCTCAGCCCCGGGAGCAACAGAGCCATCTTTGCCACTGCCATGGCTCAGG
AACTTCTGCAGAGGCCCAAACAGGAAGCAGCCATCAGCTGCTGGGTGCAGTTCAGCGACGGCTCTGTCAC
ACCCTTGGACATTTATGATGAGAAGGACTTCTCCCTGATGGCTACATCCTTGGATGAGAAGGTTGTCTCC
ATCCTCCAGGACCCCAAATTCAAGTGGCCTATCATTGCTGCAGAAAACGAAGGACAGGGGGCCCTGGTCA
AGGTTGAGATGCTCATCAGTGAATCTTGCCAGAAGTCTAAGCGGAAGAGCGTGCTGGCTGTGGGAACAGC
CAGCATCAAGGTTAAATTTGGACAGAATGATGCAAACCCCAACAGCAGTGAGAGTGGACACCTTGGGGCT
GGGCTTCATGTAGAGAATATCAATGACAGGAGGTCCAAAAAGCCTTTTCAGGAATGGGGGAGCCCCGAGG
GGCCATTCTACAGCAGCTCATCCATGGGGCTCATGGAGGGATGGGGCAGTACCACCAAGAGGCCAACTTT
CCAGAAGAAGGAAGGCCAGGAAAACCTGTTAGATGACATCATCCTCTCTCAGACCATGGCCACAGACCTC
ACCAGCTTCCCAGACCAAATGGACCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67707. Forward Primer - name:067707_F_cDNA_C630028F04Rik, sequence:GACATCACGGAGCTGATAACTG; Reverse Primer - name:067707_N_SP6_cDNA_C630028F04Rik, sequence:AGGTCCATTTGGTCTGGGAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_010351 same experiment
 EMAGE:30119 same embryo
 EMAGE:31022 same embryo
 EMAGE:30146 same embryo
 EMAGE:31059 same embryo
 EMAGE:30145 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS