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EMAGE:31058

Trh thyrotropin releasing hormone ( MGI:98823)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hypothalamus mantle layer
strong strong
single cellstrong expression: see section 07 08 09 10 11 14 15 16
trigeminal v ganglion
strong strong
single cellstrong expression: see section 02 03 04 05 06 07 17 18 19
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8025
Entity Detected:Trh, thyrotropin releasing hormone ( MGI:98823)
Sequence:sense strand is shown

>T8025
GGATTCTGGAGCCTTGCAGACTCTACCCAGCCAGTTTGCACTCTTCGGCTCAGCATCTTGGAAAGCTCTG
CAGAGTCTCCACCTTGCAGACTCCAAGATGCAGGGACCTTGGCTGATGATGGCTCTGGCTTTGATCTTCG
TGCTAACTGGTATCCCCAAATCCTGCGCCTTGCTGGAAGCAGCCCAGGAGGAAGGTGCTGTGACTCCTGA
CCTTCCAGGCCTGGAGAAAGTCCAGGTCCGGCCAGAACGTCGATTCTTGAGGAAAGACCTCCAGCGTGTG
CGAGGGGACCTTGGTGCTGCCTTAGATTCCTGGATCACAAAACGCCAGCATCCGGGCAAAAGGGAGGAGA
AGTTGGAAGACGAAGCCGAAGAGAGGGGAGATTTGGGAGAAGTGGGAGCCTGGAGACCCCACAAACGACA
GCACCCTGGCCGACGTGCCAACCAAGACAAGGATTCATGGTCAGATGAGGGGGACAGTGACTGGCTGCCC
CCGTCCTGGCTACCAGATTTCTTTCTGGATTCCTGGTTCTCAGATGCCCCCCAAGTCAAGCGGCAGCACC
CTGGCAGGCGATCCTTCCCCTGGATGGAGTCTGATGTCACCAAGAGGCAGCATCCAGGCCGGAGGTTCAT
AGATCCTGAGCTCCAAAGAAGCTGGGAAGAAACAGAGGGAGAGGAGGGTGGCTTAATGCCTGAGAAACGT
CAGCATCCTGGCAAAAGGGCAGTGGGTCACCCTTGTGGGCCCCAGGGGATTTGTGGCCAAACAGGCCTGC
TCCAGCTTCTAGGTGACCTGAGTAGGGGCCAGGAGACCCTGGCAAAGCAAACCCCACAACTGGAAGCCTG
GGTCAGGGAACCTCTGGAGGAGTAAGGTTAGAGTCAGGCTTTAGGTCTAGGCTGATGTAAGCCCTGTGTA
TCCTATCCCAGTCCCTTCACTGGTTTTCTTCTTAGATGCTAACCCTGGGCCCTCTGTACATAGCATCCAC
CCAAACCCCTCCCTTACCCACTTCAGGAACTTCAAAGAGCCAATCAGGAAGTTAAAACCCTACTTATCCC
TTCAAGCAAGGAGGTGGGGGCCCCTCTTCTCTGACAGCCCCACAGTCTCTCACCCCTTCCACACTGGCCA
AGACGGTTCTTCCACGCCTCCTAAGGGACCTCCTCAGAAAGGGTAGGATTGAAAAGTTTGGGTGTTAAAA
TCCAACTTCTGTCATCTGCCCCATATGGCAAGACTGACCTGGTATAACTTCAGCGCATCCTCCAAGGTTG
GGGGCCCTAAGCAGTTTGGGGGATATTCAGATATGTTGGGGGGGGTCACATCTTGTATATAACCCTGCTC
TTGTTAATCTCAATGACAATAAAATATATCCAGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30010395 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30010395 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006097 same experiment
 EMAGE:30638 same embryo
 EMAGE:32011 same embryo
 EMAGE:31949 same embryo
 EMAGE:31650 same embryo
 EMAGE:31973 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS