EMAGE mouse gene expression spatial database

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EMAGE:31062

Hmgcl 3-hydroxy-3-methylglutaryl-Coenzyme A lyase ( MGI:96158)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31407
Entity Detected:	Hmgcl, 3-hydroxy-3-methylglutaryl-Coenzyme A lyase ( MGI:96158)
Sequence:	sense strand is shown >T31407 AGGCTTTGAGGAAGCGGTAGCTGCGGGTGCCAAGGAAGTGAGCGTCTTCGGTGCTGTGTCTGAGCTCTTC ACCCGGAAGAACGCGAACTGCTCTATAGAGGAGAGTTTCCAGCGCTTTGCTGGAGTCATGCAGGCGGCCC AGGCCGCCAGCATCTCTGTGAGAGGGTATGTCTCCTGTGCCCTCGGATGCCCCTACGAGGGGAAGGTCTC CCCGGCTAAAGTTGCTGAGGTTGCCAAGAAGTTGTACTCGATGGGCTGCTATGAGATCTCCCTTGGGGAC ACCATCGGCGTAGGTACCCCAGGACTCATGAAAGACATGCTGACTGCTGTCATGCATGAAGTGCCTGTGA CAGCATTGGCTGTCCACTGCCATGATACCTATGGTCAAGCTCTGGCCAACACCTTGGTGGCCCTGCAGAT GGGTGTGAGTGTTGTGGACTCCTCTGTGGCAGGACTTGGAGGCTGTCCCTATGCAAAAGGGGCATCAGGG AACTTGGCTACTGAGGACCTGGTCTACATGCTGAATGGCTTAGGGATTCACACGGGTGTGAACCTCCAGA AACTTCTAGAAGCTGGGGACTTCATCTGTCAAGCCCTTAACAGAAAAACTAGTTCCAAAGTGGCACAGGC CACCTGCAAGCTCTGAGACCCTCGTTCGCCTGAACCAGAACGGAGGGAATTGGGTGTATACAATGATTCC TGGATGAAGAGATGGAATGAGAGCAAATGAGCCGGCATCACAGAGGTCCCTGTCCCTGATGATAAGGGCT AGAGCTGCCTGGCCTGGGCCAGCTCCCCAGAGCTGTGCCTAAGCACTTGCTTGGGATGGCCCTGGGTGAG TCTGCCTGCCAACAGAGCTGATGTCCACCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5049759), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 24462. Forward Primer - name:024462_F_IRAV55-58_N24_Hmgcl, sequence:AGGCTTTGAGGAAGCGGT; Reverse Primer - name:024462_R_SP6_IRAV55-58_N24_Hmgcl, sequence:GCGGTGGACATCAGCTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012527 same experiment
	EMAGE:31054 same embryo
	EMAGE:31085 same embryo
	EMAGE:30139 same embryo
	EMAGE:30148 same embryo
	EMAGE:30103 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source