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EMAGE:31085

Cgref1 ( MGI:1915817)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31085 EMAGE:31085 EMAGE:31085 EMAGE:31085 EMAGE:31085
euxassay_012517_01 euxassay_012517_02 euxassay_012517_03 euxassay_012517_04 euxassay_012517_05
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euxassay_012517_21 euxassay_012517_22 euxassay_012517_23 euxassay_012517_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
mandible
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22
ventral grey horn
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16
heart ventricle
moderate moderate
spottedmoderate expression: see section 10 11 12 13 14 15 16 17 18
vault of skull
strong strong
regionalstrong expression: see section 23 24
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 23 24
clavicle
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 17 18 19 20
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 18 19 20 21 22 23
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 18 19
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 16
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 19 20
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 08 17 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22
scapula
strong strong
regionalstrong expression: see section 03 04 23
femur
strong strong
regionalstrong expression: see section 01 16 17 18
maxilla
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 20 21 22 23 24
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31373
Entity Detected:Cgref1, ( MGI:1915817)
Sequence:sense strand is shown

>T31373
CCTGAGCAGCTCCGACATCTGCAGAATTATCTCAAGGGACTAGAGAAGATGGAAGAGGATCCTGAGCACA
TGGACCGGGAGCAAGTCCTGCTTTCGCTCTTTGCTCTTCATGACTATGACCAGAATGGACAGCTGGACGG
CCTGGAGCTACTGTCCATGCTGACAGCAGCTCTGGCCCCTGGAGCTGCACACTTTCCCATCAACCCGGTG
ATCCTAGTAGTAGACTCGGTGCTTGAGACTCAGGACCTGGATGGAGACGGGCTCATGACTCCTGCAGAGC
TCATCAACTTCCCAGAAGTCCCCAAACACACAGAGTCCCTTCCCCCAGCTCTCCAGGAGCCACAACCTGC
TGGAAGTCAGCCACTTTTAGCCAACAGTCCACTGCAGTCAGAAACTCAGCAGTCTCTGGGGACTAAAGAA
GAAATTAGGGGCCAGGTAGAGGCCAAGAGGGCGTCCTTGGAGCCTGAACAGGAGGCTGGACATCAGACAG
AGGGAAAAGTAGATACCCTAAGCCCTGAAGAGGAGGCTAGGGGACAGGCAGAGTCTGAAGGAGATGTCCC
AGGTCCCAGAGAAGGTGCTGAGGAACAGGTGGAGATCAAGGACAATGAAGGAGAAGCCAAAGAACTGCTG
GTGGAAACACTGGAGAGCCTAAACACTCCAAATGAGGCTGAGGCTCATAGCATCCAATTGGAGAACGATG
AGATATGAGCCCGACGGCATAGGCTCAAGCCCCTCAGAATCTCAGTGCAGAGCAGAAGCATGGTGTTGAA
TATGGTGGGGTTAGAGCCACCTCTGACATGGGGACGGTGGTGCAAGCTAGTAACCCCAGAAGCATTGAGA
GCTCCGGGCTAGCCTAGGCCACATAGCAAGTTTGAAGCCAGGCTGGGCTACTGCGTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4206019), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22866. Forward Primer - name:022866_F_IRAV55-58_E19_Cgref1, sequence:CCTGAGCAGCTCCGACAT; Reverse Primer - name:022866_R_SP6_IRAV55-58_E19_Cgref1, sequence:TTACGCAGTAGCCCAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012517 same experiment
 EMAGE:31062 same embryo
 EMAGE:31054 same embryo
 EMAGE:30139 same embryo
 EMAGE:30148 same embryo
 EMAGE:30103 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS