EMAGE mouse gene expression spatial database

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EMAGE:31116

Myoz2 ( MGI:1913063)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000748_01	euxassay_000748_02	euxassay_000748_03	euxassay_000748_04	euxassay_000748_05

euxassay_000748_06	euxassay_000748_07	euxassay_000748_08	euxassay_000748_09	euxassay_000748_10

euxassay_000748_11	euxassay_000748_12	euxassay_000748_13	euxassay_000748_14	euxassay_000748_15

euxassay_000748_16	euxassay_000748_17	euxassay_000748_18	euxassay_000748_19	euxassay_000748_20

euxassay_000748_21	euxassay_000748_22	euxassay_000748_23	euxassay_000748_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 13 14 15 17 18 19

diaphragm

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23 24

tail

weak

regional

weak expression: see section 13 14 15 16 17 18 19

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 weak expression: see section 07 22 23 24

tongue

moderate

regional

moderate expression: see section 12 13 14 15 16 17 weak expression: see section 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1022
Entity Detected:	Myoz2, ( MGI:1913063)
Sequence:	sense strand is shown >T1022 TCCTCGAGCCTGTTGGCCTACTGGGTTTGAGTCAGATCGAGGGACCATGCCGTTCCAGGTTCAAGGATAA AACCCATTGGGCCCAAGTGCCGTCATATTCCACCTTCAGTGCCTTCCTCCACAATTGGGATTCACCCCTG CTGAAAAGCGCAGCCTGACAGCAAGGGAACAAAAAACTATGCTATCACATAGTGCCATGGTGAGGCAAAG GAAACAGCAAGCATCAGCCATCACGAAGGAAATCCATGGACATGATGTTGACGGCATGGACCTGGGCAAA AAAGTTAGCATCCCCAGAGACATCATGATAGAAGAATTGTCCCATTTCAGTAATCGTGGGGCCAGGCTGT TTAAGATGCGTCAAAGAAGATCTGACAAATACACCTTTGAAAATTTCCAGTATGAATCTAGAGCACAAAT TAATCACAATATCGCCATGCAGAATGGGAGAGTTGATGGAAGCAACCTGGAAGGTGGCTCACAGCAAGGC CCCTCAACTCCGCCCAACACCCCCGATCCACGAAGCCCCCCAAATCCAGAGAACATCGCACCAGGATAT
Notes:	The probe template was PCR amplified from IMAGE:2076683 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2076683 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000748 same experiment
	EMAGE:31131 same embryo
	EMAGE:31112 same embryo
	EMAGE:31133 same embryo
	EMAGE:31129 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source