EMAGE mouse gene expression spatial database

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EMAGE:31121

Tex2 ( MGI:102465)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007131_01	euxassay_007131_02	euxassay_007131_03	euxassay_007131_04	euxassay_007131_05

euxassay_007131_06	euxassay_007131_07	euxassay_007131_08	euxassay_007131_09	euxassay_007131_10

euxassay_007131_11	euxassay_007131_12	euxassay_007131_13	euxassay_007131_14	euxassay_007131_15

euxassay_007131_16	euxassay_007131_17	euxassay_007131_18	euxassay_007131_19	euxassay_007131_20

euxassay_007131_21	euxassay_007131_22	euxassay_007131_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 20 21 22 23

liver right lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 moderate expression: see section 08 16 17

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v nerve

strong

regional

strong expression: see section 10

testis

strong

regional

strong expression: see section 06 07 08 09 16 17 18

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

thyroid gland

moderate

regional

moderate expression: see section 10 11 12 14 15

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 13 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 18 19 20

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18

vagus x ganglion

strong

regional

strong expression: see section 08 16 moderate expression: see section 09

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 16 17 18 19 20 21

vibrissa

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8409
Entity Detected:	Tex2, ( MGI:102465)
Sequence:	sense strand is shown >T8409 TGGGCGTCGGCCTCGGCTTCATGACTGCAGTCTGCATGATTTGGTTCTTTACACCACCAAGTGCTCACAA ACACCACAAATCACTCAAAGCTCTGCGGCACCAGAGCACGAGATCCCTGGACATCAAGGAGCCCGAAATA CTAAAGGGATGGATGAATGAGATTTACAACTATGATCCAGAAACTTACCACGCGACTTTGACGCACTCAG TCTTTGTCCGGCTCGAGGGTGGAACCTTAAGACTTTCAAAGCCCAACAAAAACATATCCAGGAGGGCAAG CTACAATGAGACAAAGCCAGAGGTCACCTACATCAGCCAGAAAATCTATGACCTCTCAGACAGCAAGATT TATCTTGTACCTAAAAGCTTGGCTCGAAAGCGAATCTGGAATAAGAAGTACCCCATTTGCATTGAACTTG GTCGGCAAGATGACTTTATGTCTAAGGCGCAGTCTGATAAGGAAGCTACTGAGGAGAAGCCGCCTCCTGA GAAAGAGCTGCCGAGCGAAGACCTCAAGAAGCCACCCCAGCCTCAAGAGGGGACGAAGTCTAGCCAGCGA GACCCAATACTGTATCTGTTTGGGAGAACGGGCCGAGAGAAAGAGGAGTGGTTCAGGCGGTTCATCCTGG CCTCGAGGCTGAAGTCAGAGCTCAGGAAGCCGGCGGGTGTTTCTGGAAGTAAATCAGGGCTTCTGCCCGC ACACAGCAGGCACAGCAGTCCCTCGGGGCACCTGAGCCACAGCCGCAGCAGCAGCAAAGGCAGCGTGGAG GAAATGATGTCCCAGCCAAAGCAGAAGGAACTGGTGGGCAGCGTGCGACAGAAGATGCTCCTAGACTACA GTGTGTACATGGGCAGGTGTGTGCCCCAGGACAACCGAAGCCCCCACAGGAGCCCCGTGCAGAGTGCAGA GAGCAGCCCCACAGCTTCGAAGAAGTTGCCAGAGGCCCCACCCTCTGAGGAGGAAGAACAGGAAGCCTGG GTGAATGCTTTGCTTGGAAGAATATTTTGGGACTTCTTGGGCGAGAAATACTGGTCTGATGTGGTGTCGA AGAAAATCCAGATGAAACTCAGCAAAATTAAGCTCCCCTACTTTATGAATGAACTAACACTGACAGAGCT TGACATGGGTGTAGCTGTGCCAAAAATCCTTCAGGCCTTCAAGCCTTATGTTGATCACCAAGGACTCTGG ATCGATTTGGAAATGTCTTACAATGGGTCCTTTCTGATGACTCTGGAAACCAAAATGAACTTGACCAAAC TAGGTAAAGAGCCTCTTGTTGAAGCCCTGAAGGTTGGAGAAATTGGCAAAGAAGGGCCCAGGGCATACTG TCTGGCTGACAGTGATGAAGAATCCTCCAGTGCTGGCTCCTCTGAGGAAGATGACCCACCAGAACCCACT GCTGGAGACAAACAGCCGCTCCCCGGGGCTGAAGGGTACGTTGGAGGTCATCGAACAAGCAAGATTATGA GGTTTGTTGATAAGATCACCAAATCAAAATATTTCCAAAAAGCAACAGAGACAGAATTCATTAAAAAGAA GATCGAAGAAGTCTCTAATACACCACTGCTGCTAACTGTGGAAGTGCAAGAGTGTCGAGGGACCTTGGCG GTCAACATCCCCCCACCACCGACTGACCGGATATGGTATGGTTTCCGGAAGCCACCGTATGTGGAGCTGA AAGCTCGGCCAAAACTTGGAGAGAGAGAAGTGACTTTAGTTCACGTGACAGAGTGGATTGAGAAGAAACT GGAGCAGGAGCTTCAGAAAGTGTTTGTCATGCCAAACATGGATGACGTTTATATTCCTATAATGCACTCG GCCATGGACCCTCGCTCCACTTCCTGCCTCTTGAAAGAGCCACCTGTGGAGACCTCTGATCAGCTGTGAT TGGTGAAGATGGGACATTCCCAGTATTATGAGACCTAGCTCAAGGTGTGGGATCTGTAGCTGCCATCTGT TCTGTGGGGGTGGCCTTTCCTGGTGCCACAGCTACTGTCTCTTAAAGGACTGCTTCCGCCCTCTGCCTGC TGGCCCATTCCACTGAGGTGTCATTCCCTGCATCTGGTGACTAGTATCTGGTTTGTCTGATGCAAAGCTT TGACTGGCAAAGGAGACTTGAACTATAATGGTAGAGCCAGAGTTACAGCTGACTCGCACCATGGCTTTTT TTTTTTTTTTTTAAGTCTACCAGGTTTTATGACAGCAAATGAGCACATTCAGAGCAAAGCTGTACAACTA GAAGGCTTCCCTTTGCCTTTTGACTCCTCCAAAGCTTTTCCTCAGGCAGCTGGTGCACAATGGAACATTT TTCCACTCTACACTTTGTACGCATGACTGCGGCCCTCCATCCTTGGAGAATGTCACTGTGGTAACCTGTG TGGAGTGTGGTTTTGTGAGCCATTTCATTGGTGGTAAAATAGGCATAGCAACTTTTTTCATATTAAATTG TGCATTTTGGAAGCAAGTAGCCATAGAACATACACACCCACACTTTCACTGGGGTGGGCTTCATGTGAAC ATCAGGCAAAGCCGTGAGATCAAACCATCCCAAAGACGTACAATCGCCTTGCTTTTCAACCAATCCTGAC TCTTCTGAGACATGAGCGAGTGTGACAATGTCAAAACACTGTGCACAACGACCGATTGTCCCTCCCTGGT ACTGATGGGACGGGGCCAGAAAGTGGGAGCCAAAGTGTTCTTTTCTGGTTTTTCTGTGTGCTGTAGGATG ATCGAACGGGTTCGGGAGGTTTGTGGGCAGTCCCCTTCTCTGGTGGCCAAATGGACTTTCCTTTCTCCTC AGTGGCAAGCACTACATCCACACATGCACTTGTGACCTTTCTTTAATGTTGCCAACTTACGTTTGTGCAT TTGTGTCAGAATGTTTAGTTTACAAACTCGGGGGCTCTAATTGTATAATTTGCTTTGAGGAGATCTACTA GGTTTTCTTGTTTGTTTAAAGCTGCATCCAATGTCAAAATTACCTTGAGTAACTTCTGATAATTTACTGT CTGTGGACAAAGCTAAGTTTTTTTCTTAAACAGCACCTTGCTTGAAGATGACTTTAAAGAAATTAATATA TTGAAAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5687258 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5687258 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007131 same experiment
	EMAGE:29728 same embryo
	EMAGE:30859 same embryo
	EMAGE:31730 same embryo
	EMAGE:31821 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source