EMAGE mouse gene expression spatial database

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EMAGE:31131

Vkorc1 ( MGI:106442)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000753_01	euxassay_000753_02	euxassay_000753_03	euxassay_000753_04	euxassay_000753_05

euxassay_000753_06	euxassay_000753_07	euxassay_000753_08	euxassay_000753_09	euxassay_000753_10

euxassay_000753_11	euxassay_000753_12	euxassay_000753_13	euxassay_000753_14	euxassay_000753_15

euxassay_000753_16	euxassay_000753_17	euxassay_000753_18	euxassay_000753_19	euxassay_000753_20

euxassay_000753_21	euxassay_000753_22	euxassay_000753_23	euxassay_000753_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 19 20 21 22 23 24

upper jaw molar

weak

regional

weak expression: see section 07 08 09 10 11 19 20 21 22 23 24

upper jaw incisor

weak

regional

weak expression: see section 09 10 11 12 13 14 17 18 19 20 21

chondrocranium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 weak expression: see section 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1021
Entity Detected:	Vkorc1, ( MGI:106442)
Sequence:	sense strand is shown >T1021 TCTCAGNCTGTTGGCCTACTGGTTCTTCCCTCCTGTCCCTGGGCTGTACTGTCGACATGGGCACCACCTG GAGGAGCCCTGGACTCGTGCGGCTTGCACTGTGCCTCGCTGGCTTAGCCCTCTCACTGTACGCACTGCAC GTGAAGGCGGCGCGCGCCCGCGATGAAAATTACCGCGCGCTCTGCGATGTGGGCACGGCCATCAGCTGTT CCCGCGTCTTCTCCTCTCGGTGGGGCCGGGGCTTTGGGCTGGTGGAGCACATGCTAGGAGCGGACAGCGT CCTCAACCAATCCAACAGCATATTTGGTTGCCTGTTCTACACCTTACAGCTGTTGTTAGGTTGCTTGAGG GGACGTTGGGCCTCTATCCTACTGGTGCTGAGTTCCCTGGTGTCCGTCGCTGGTTCCGTGTACCTGGCCT GGATCCTGTTCTTTGTGTTATATGATTTCTGCATTGTGTGCATTACCACCTATGCCATCAATGTGGGTCT GATGTTGCTTAGCTTCCAGAAGGTACCAGAACACAAGACCAAAAAGCACTGA
Notes:	The probe template was PCR amplified from IMAGE:2076623 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2076623 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000753 same experiment
	EMAGE:31112 same embryo
	EMAGE:31133 same embryo
	EMAGE:31129 same embryo
	EMAGE:31116 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source