EMAGE mouse gene expression spatial database

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EMAGE:31150

Ada adenosine deaminase ( MGI:87916)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 12 13 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5961
Entity Detected:	Ada, adenosine deaminase ( MGI:87916)
Sequence:	sense strand is shown >T5961 GCCGCAGTCTCTGAGAGCCATCGGGAAGCGACCCCGCCAGCGAGCCAACGCAGACCCAGAGAGCTTCGGC GGAGAGAACCGGGAGCACGCTCGGAACCATGGCCCAGACGCCCGCATTCAACAAACCCAAAGTAGAGTTA CACGTCCACCTGGATGGAGCCATCAAGCCAGAAACCATCTTATACTTTGGCAAGAAGAGAGGCATCGCCC TCCCGGCAGATACAGTGGAGGAGCTGCGCAACATTATCGGCATGGACAAGCCCCTCTCGCTCCCAGGCTT CCTGGCCAAGTTTGACTACTACATGCCTGTGATTGCGGGCTGCAGAGAGGCCATCAAGAGGATCGCCTAC GAGTTTGTGGAGATGAAGGCAAAGGAGGGCGTGGTCTATGTGGAAGTGCGCTATAGCCCACACCTGCTGG CCAATTCCAAGGTGGACCCAATGCCCTGGAACCAGACTGAAGGGGACGTCACCCCTGATGACGTTGTGGA TCTTGTGAACCAGGGCCTGCAGGAGGGAGAGCAAGCATTCGGCATCAAGGTCCGGTCCATTCTGTGCTGC ATGCGCCACCAGCCCAGCTGGTCCCTTGAAGTGTTGGAGCTGTGTAAGAAGTACAATCAGAAGACCGTGG TGGCTATGGACTTGGCTGGGGATGAGACCATTGAAGGAAGTAGCCTCTTCCCAGGCCACGTGGAAGCCTA TGAGGGCGCAGTAAAGAATGGCATTCATCGGACCGTCCACGCTGGCGAGGTGGGCTCTCCTGAGGTTGTG CGCGAGGCTGTGGACATCCTCAAGACAGAGAGGGTGGGACATGGTTACCACACCATCGAGGATGAAGCCC TCTACAACAGGCTACTGAAAGAAAACATGCACTTTGAGGTCTGCCCCTGGTCCAGCTACCTCACAGGCGC CTGGGATCCCAAAACGACGCATGCGGTTGTTCGCTTCAAGAATGATAAGGCCAACTACTCACTCAACACA GACGACCCCCTCATCTTCAAGTCTACTCTAGACACTGACTACCAGATGACCAAGAAAGACATGGGCTTCA CTGAGGAGGAGTTCAAGCGACTGAACATCAACGCAGCGAAGTCAAGCTTCCTCCCAGAGGAAGAGAAGAA GGAACTTCTGGAACGGCTCTACAGAGAATACCAATAGCCACCACAGGCTGACGGGCAGGTCCCCTGAAGA TGGCAAGGCCACTTCTCTGAGCCTCATCCTGTGGATAAAGTCTTTACAACTCTGACATACTGACCTTCAT TCCTTCCAGACCTTGGAGAGGCCAGGTCTGTCCTCTGATTGGATATCCTGGCTAGGTCCCAGGGGCCTTG ACAATCATGCACATGAATTGAAAACCTTCTTTCTAAAGCTAAAATTATGGTGTTCAATAAAGCAGCTGGT GACTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3484465 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484465 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003318 same experiment
	EMAGE:31198 same embryo
	EMAGE:31152 same embryo
	EMAGE:29396 same embryo
	EMAGE:31466 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source