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EMAGE:31169

Prkar2b ( MGI:97760)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31169 EMAGE:31169 EMAGE:31169 EMAGE:31169 EMAGE:31169
euxassay_012279_01 euxassay_012279_02 euxassay_012279_03 euxassay_012279_04 euxassay_012279_05
EMAGE:31169 EMAGE:31169 EMAGE:31169 EMAGE:31169 EMAGE:31169
euxassay_012279_06 euxassay_012279_07 euxassay_012279_08 euxassay_012279_09 euxassay_012279_10
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euxassay_012279_11 euxassay_012279_12 euxassay_012279_13 euxassay_012279_14 euxassay_012279_15
EMAGE:31169 EMAGE:31169 EMAGE:31169 EMAGE:31169 EMAGE:31169
euxassay_012279_16 euxassay_012279_17 euxassay_012279_18 euxassay_012279_19 euxassay_012279_20
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euxassay_012279_21 euxassay_012279_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
neural retina
moderate moderate
regionalmoderate expression: see section 01 20 21 22
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 17 18
spinal cord
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 17 18
testis
strong strong
regionalstrong expression: see section 06 07 08 17 18 19 20
tongue muscle
weak weak
regionalweak expression: see section 08 09 10 11 12 13
telencephalon
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 16 17 18 19 weak expression: see section 10 11 14
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 19 20
submandibular gland primordium
weak weak
regionalweak expression: see section 07 08 14 16 17
midbrain
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
diencephalon
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 14 17 18 19 20 21
adrenal gland
strong strong
regionalstrong expression: see section 09 10 14 16 17 18
vibrissa
moderate moderate
regionalmoderate expression: see section 17 18 19 weak expression: see section 02 03
liver lobe
moderate moderate
regionalmoderate expression: see section 01 03 04 05 12 13 14 15 16 17 19 20 21 22 weak expression: see section 02 06 07 08 09 10 11 18
hindbrain
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30438
Entity Detected:Prkar2b, ( MGI:97760)
Sequence:sense strand is shown

>T30438
CAGGAGAACGAGCGCAAGGGCGCCGCGCGCTTCGGCCATGAGGGCAGGACCTGGGGGGACGCGGGCGCAG
CCGCCGGGGGCGGCACCCCCAGTAAGGGTGTCAACTTCGCCGAGGAGCCCATGCGCTCCGACTCCGAGAA
CGGCGAGGAGGAGGAGGCCGCGGAAGCAGGGGCGTTCAACGCTCCAGTTATAAACCGGTTCACAAGGCGT
GCCTCGGTCTGTGCAGAAGCTTATAATCCTGATGAAGAGGAAGACGATGCAGAGTCCAGGATAATACATC
CCAAAACCGATGATCAGAGAAACAGATTGCAAGAGGCTTGCAAAGACATCCTGCTGTTTAAGAACCTGGA
TCCAGAGCAGATGTCCCAAGTTTTAGATGCCATGTTTGAAAAATTGGTCAAAGAAGGGGAACACGTAATT
GACCAAGGTGATGACGGTGACAACTTTTACGTCATTGACAGAGGAACATTTGATATTTATGTAAAATGTG
ATGGCGTTGGAAGATGCGTCGGTAACTATGATAACCGTGGGAGTTTTGGAGAACTGGCCTTAATGTACAA
TACACCCAGAGCAGCTACAATCACTGCTACCTCTCCTGGTGCTCTGTGGGGTTTGGACAGGGTGACCTTC
AGGAGAATAATTGTAAAAAACAATGCCAAAAAGAGGAAGATGTACGAGAGCTTTATCGAGTCCCTGCCGT
TCCTCAAGTCTCTAGAGGTTTCTGAACGCCTGAAGGTGGTGGATGTGATTGGCACCAAAGTTTACAATGA
TGGAGAGCAGATCATTGCTCAGGGAGATTTGGCAGACTCTTTCTTCATTGTAGAATCTGGAGAAGTGAAA
ATTACTATGAAAAGAAAGGGTAAATCAGAGGTTGAAGAGAACGGAGCTGTGGAAATCGCTCGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6506827), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58615. Forward Primer - name:058615_F_IRAV105_e07_Prkar2b, sequence:CAGGAGAACGAGCGCAAG; Reverse Primer - name:058615_R_SP6_IRAV105_e07_Prkar2b, sequence:ACCGAGCGATTTCCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012279 same experiment
 EMAGE:31191 same embryo
 EMAGE:31473 same embryo
 EMAGE:32005 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS