EMAGE mouse gene expression spatial database

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EMAGE:31181

Tnnt2 troponin T2, cardiac ( MGI:104597)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000294_01	euxassay_000294_02	euxassay_000294_03	euxassay_000294_04	euxassay_000294_05

euxassay_000294_06	euxassay_000294_07	euxassay_000294_08	euxassay_000294_09	euxassay_000294_10

euxassay_000294_11	euxassay_000294_12	euxassay_000294_13	euxassay_000294_14	euxassay_000294_15

euxassay_000294_16	euxassay_000294_17	euxassay_000294_18	euxassay_000294_19	euxassay_000294_20

euxassay_000294_21	euxassay_000294_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

right atrium cardiac muscle

strong

regional

strong expression: see section 05 06 07 08 09 11 12 13 14 15 16

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22

tail mesenchyme

strong

regional

strong expression: see section 09 10 11 12 13 14

left atrium cardiac muscle

strong

regional

strong expression: see section 05 06 07 08 09 11 12 13 14 15 16

heart ventricle

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13

tongue

moderate

regional

moderate expression: see section 09 10 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3683
Entity Detected:	Tnnt2, troponin T2, cardiac ( MGI:104597)
Sequence:	sense strand is shown >T3683 TGGCCTCGAGCCAGATTCGGCACGAGGGGAAGAGACAGACAGAGAGAGAGAAGAAGAAGAAGATCCTGGC AGAGAGGAGGAAGGCGCTGGCCATCGACCACCTGAATGAAGACCAACTGAGAGAGAAGGCCAAGGAGCTG TGGCAGAGTATTCACAACCTGGAGGCTGAGAAGTTCGACCTGCAGGAAAAGTTCAAGCAGCAGAAATACG AAATCAACGTTCTGCGAAACCGGATCAATGACAACCAGAAAGTCTCCAAAACTCGTGGGAAGGCCAAAGT CACCGGGCGTTGGAAATAGATGAAACTGTTCTCGTCAAAGCTGTGCCCCCTGCTTGTGTCCTTGCCCCGT GCATCCCAGCTCTGGGTCCTCCTTGGCACCCAATGCAGACTCCTGTTTGGATAGTGGGAGCTGGCTTAGC TAGAAGCCAGTACTCTGCCTGACCCCTATGCCCGCACTATGCCAGCAATAAAAAGCCAACACACTGCAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:353389 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:353389 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000294 same experiment
	EMAGE:29381 same embryo
	EMAGE:29850 same embryo
	EMAGE:29379 same embryo
	EMAGE:29411 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source