EMAGE mouse gene expression spatial database

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EMAGE:31184

Stat3 signal transducer and activator of transcription 3 ( MGI:103038)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004868_01	euxassay_004868_02	euxassay_004868_03	euxassay_004868_04	euxassay_004868_05

euxassay_004868_06	euxassay_004868_07	euxassay_004868_08	euxassay_004868_09	euxassay_004868_10

euxassay_004868_11	euxassay_004868_12	euxassay_004868_13	euxassay_004868_14	euxassay_004868_15

euxassay_004868_16	euxassay_004868_17	euxassay_004868_18	euxassay_004868_19	euxassay_004868_20

euxassay_004868_21	euxassay_004868_22	euxassay_004868_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 19 20

ventral grey horn

strong

regional

strong expression: see section 06 07 09 10 11 12

hindlimb digit 1 mesenchyme

strong

regional

strong expression: see section 04 moderate expression: see section 18 19

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 05 06 19 20

axial skeleton tail region

strong

regional

strong expression: see section 11

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 15

hindlimb digit 2 mesenchyme

strong

regional

strong expression: see section 04 moderate expression: see section 05 06 19 20

pons mantle layer

strong

regional

strong expression: see section 07 16

dorsal grey horn

strong

regional

strong expression: see section 07 08

choroid invagination

strong

regional

strong expression: see section 19

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 11 12 13 14

cerebral cortex ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 13 14

facial vii ganglion

strong

regional

strong expression: see section 04 05 18 19 20

hand mesenchyme

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 21 22 23

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 05 06 19 20

thymus primordium

strong

homogeneous

strong expression: see section 08 09 10 11 12

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6568
Entity Detected:	Stat3, signal transducer and activator of transcription 3 ( MGI:103038)
Sequence:	sense strand is shown >T6568 CCCACGCGTCCGCGCTGAGGTACAACCCCGCTCGGTGTCGCCTGACCGCGTCGGCTAGGAGAGGCCAGGC GGCCCTCGGGAGCCCAGCAGCTCGCGCCTGGAGTCAGCGCAGGCCGGCCAGTCGGGCCTCAGCCCCGGAG ACAGTCGAGACCCCTGACTGCAGCAGGATGGCTCAGTGGAACCAGCTGCAGCAGCTGGACACACGCTACC TGGAGCAGCTGCACCAGCTGTACAGCGACAGCTTCCCCATGGAGCTGCGGCAGTTCCTGGCACCTTGGAT TGAGAGTCAAGACTGGGCATATGCAGCCAGCAAAGAGTCACATGCCACGTTGGTGTTTCATAATCTCTTG GGTGAAATTGACCAGCAATATAGCCGATTCCTGCAAGAGTCCAATGTCCTCTATCAGCACAACCTTCGAA GAATCAAGCAGTTTCTGCAGAGCAGGTATCTTGAGAAGCCAATGGAAATTGCCCGGATCGTGGCCCGATG CCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACGGCAGCCCAGCAAGGGGGCCAGGCCAACCAC CCAACAGCCGCCGTAGTGACAGAGAAGCAGCAGATGTTGGAGCAGCATCTTCAGGATGTCCGGAAGCGAG TGCAGGATCTAGAACAGAAAATGAAGGTGGTGGAGAACCTCCAGGACGACTTTGATTTCAACTACAAAAC CCTCAAGAGCCAAGGAGACATGCAGGATCTGAATGGAAACAACCAGTCTGTGACCAGACAGAAGATGCAG CAGCTGGAACAGATGCTCACAGCCCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCTCT TGTCAGCAATGGAGTACGTGCAGAAGACACTGACTGATGAAGAGCTGGCTGACTGGAAGAGGCGGCAGCA GATCGCGTGCATCGGAGGCCCTCCCAACATCTGCCTGGACCGTCTGGAAAACTGGATAACTTCATTAGCA GAATCTCAACTTCAGACCCGCCAACAAATTAAGAAACTGGAGGAGCTGCAGCAGAAAGTGTCCTACAAGG GCGACCCTATCGTGCAGCACCGGCCCATGCTGGAGGAGAGGATCGTGGAGCTGTTCAGAAACTTAATGAA GAGTGCCTTCGTGGTGGAGCGGCAGCCCTGCATGCCCATGCACCCGGACCGGCCCTTAGTCATCAAGACT GGTGTCCAGTTTACCACGAAAGTCAGGTTGCTGGTCAAATTTCCTGAGTTGAATTATCAGCTTAAAATTA AAGTGTGCATTGATAAAGACTCTGGGGATGTTGCTGCCCTCAGAGGGTCTCGGAAATTTAACATTCTGGG CACGAACACAAAAGTGATGAACATGGAGGAGTCTAACAACGGCAGCCTGTCTGCAGAGTTCAAGCACCTG ACCCTTAGGGAGCAGAGATGTGGGAATGGAGGCCGTGCCAATTGTGATGCCTCCTTGATCGTGACTGAGG AGCTGCACCTGATCACCTTCGAGACTGAGGTGTACCACCAAGGCCTCAAGATTGACCTAGAGACCCACTC CTTGCCAGTTGTGGTGATCTCCAACATCTGTCAGATGCCAAATGCTTGGGCATCAATCCTGTGGTATAAC ATGCTGACCAATAACCCCAAGAACGTGAACTTCTTCACTAAGCCGCCAATTGGAACCTGGGACCAAGTGG CCGAGGTGCTCAGCTGGCAGTTCTCGTCCACCACCAAGCGGGGGCTGAGCATCGAGCAGCTGACAACGCT GGCTGAGAAGCTCCTAGGGCCTGGTGTGAACTACTCAGGGTGTCAGATCACATGGGCTAAATTCTGCAAA GAAAACATGGCTGGCAAGGGCTTCTCCTTCTGGGTCTGGCTAGACAATATCATCGACCTTGTGAAAAAGT ATATCTTGGCCCTTTGGAATGAAGGGTACATCATGGGTTTCATCAGCAAGGAGCGGGAGCGGGCCATCCT AAGCACAAAGCCCCCGGGCACCTTCCTACTGCGCTTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTC ACTTGGGTGGAAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCATACACCAAGCAGCAGC TGAACAACATGTCATTTGCTGAAATCATCATGGGCTATAAGATCATGGATGCGACCAACATCCTGGTGTC TCCACTTGTCTACCTCTACCCCGACATTCCCAAGGAGGAGGCATTTGGAAAGTACTGTAGGCCCGAGAGC CAGGAGCACCCCGAAGCCGACCCAGGTAGTGCTGCCCCGTACCTGAAGACCAAGTTCATCTGTGTGACAC CAACGACCTGCAGCAATACCATTGACCTGCCGATGTCCCCCCGCACTTTAGATTCATTGATGCAGTTTGG AAATAACGGTGAAGGTGCTGAGCCCTCAGCAGGAGGGCAGTTTGAGTCGCTCACGTTTGACATGGATCTG ACCTCGGAGTGTGCTACCTCCCCCATGTGAGGAGCTGAAACCAGAAGCTGCAGAGACGTGACTTGAGACA CCTGCCCCGTGCTCCACCCCTAAGCAGCCGAACCCCATATCGTCTGAAACTCCTAACTTTGTGGTTCCAG ATTTTTTTTTTTAATTTCCTACTTCTGCTATCTTTGGGCAATCTGGGCACTTTTTAAAATAGAGAAATGA GTGAGTGTGGGTGATAAACTGTTATGTAAAGAGGAGAGCACCTCTGAGTCTGGGGATGGGGCTGAGAGCA GAAGGGAGCAAGGGGAACACCTCCTGTCCTGCCCGCCTGCCCTCCTTTTTCAGCAGCTCGGGGTTGGTTG TTAGACAAGTGCCTCCTGGTGCCCATGGCATCCTGTTGCCCCACTCTGTGAGCTGATACCCCAGGCTGGG AACTCCTGGCTCTGCACTTTCAACCTTGCTAATATCCACATAGAAGCTAGGACTAAGCCCAGAGGTTCCT CTTTAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3593588 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593588 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004868 same experiment
	EMAGE:31606 same embryo
	EMAGE:30901 same embryo
	EMAGE:31615 same embryo
	EMAGE:31026 same embryo
	EMAGE:30216 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source