Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31189

Arid3b AT rich interactive domain 3B (BRIGHT-like) ( MGI:1930768)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31189 EMAGE:31189 EMAGE:31189 EMAGE:31189 EMAGE:31189
euxassay_012254_01 euxassay_012254_02 euxassay_012254_03 euxassay_012254_04 euxassay_012254_05
EMAGE:31189 EMAGE:31189 EMAGE:31189 EMAGE:31189 EMAGE:31189
euxassay_012254_06 euxassay_012254_07 euxassay_012254_08 euxassay_012254_09 euxassay_012254_10
EMAGE:31189 EMAGE:31189 EMAGE:31189 EMAGE:31189 EMAGE:31189
euxassay_012254_11 euxassay_012254_12 euxassay_012254_13 euxassay_012254_14 euxassay_012254_15
EMAGE:31189 EMAGE:31189 EMAGE:31189 EMAGE:31189 EMAGE:31189
euxassay_012254_16 euxassay_012254_17 euxassay_012254_18 euxassay_012254_19 euxassay_012254_20
EMAGE:31189 EMAGE:31189 EMAGE:31189
euxassay_012254_21 euxassay_012254_22 euxassay_012254_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lens
weak weak
regionalweak expression: see section 22 23
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30396
Entity Detected:Arid3b, AT rich interactive domain 3B (BRIGHT-like) ( MGI:1930768)
Sequence:sense strand is shown

>T30396
ATCACACCCGGGAGACCTTCTGGTAGTTCTGTTCTGGGTCCCTTAGCCAGAGTTCCACCAGCCACACCGG
TGGCTCGAATGTCTGAGCAGAGCAACGTGAACTCCGAGCCTGAGGAGGAGGAAGGAGGCCTGGAAGATGA
AGATGGAGATGATGATGTTGCAGAGGTGGCTGAAAAAGAGGCCCAGGCTGCTTCCAAATATTTCCACATG
CAAAAAGTAACTCGCCAAGAACCCAGAGCAACACCCATGTCCAGCCTGCTTCCAGTACCCGGACTTTCAC
CACAGGGACAACAAACTAAAGAAGACCATACCAAAGATGCTTCCAAGGCCCCGCCCTCTGTGCCCACAGC
TGGACAGCCGAGCTGGAGTCTGGATGAGCAGCTTAAGCAGAATGGTGCTTTGGCCTGGAGTGACGATGCT
GATGGAGGCAGGGGAAGAGAGATCTCTCGAGATTTTGCCAAGCTGTATGAACTAGACGGTGATCCTGAAA
GGAAAGAATTTCTGGATGACCTCTTCATCTTCATGCAGAAGAGAGGCACCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6394061), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58532. Forward Primer - name:058532_F_IRAV103_b07_Arid3b, sequence:ATCACACCCGGGAGACCT; Reverse Primer - name:058532_R_SP6_IRAV103_b07_Arid3b, sequence:TGGGGTGCCTCTCTTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012254 same experiment
 EMAGE:31095 same embryo
 EMAGE:31165 same embryo
 EMAGE:29388 same embryo
 EMAGE:31167 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS