EMAGE mouse gene expression spatial database

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EMAGE:31193

Acsl6 acyl-CoA synthetase long-chain family member 6 ( MGI:894291)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002680_01	euxassay_002680_02	euxassay_002680_03	euxassay_002680_04	euxassay_002680_05

euxassay_002680_06	euxassay_002680_07	euxassay_002680_08	euxassay_002680_09	euxassay_002680_10

euxassay_002680_11	euxassay_002680_12	euxassay_002680_13	euxassay_002680_14	euxassay_002680_15

euxassay_002680_16	euxassay_002680_17	euxassay_002680_18	euxassay_002680_19	euxassay_002680_20

euxassay_002680_21	euxassay_002680_22	euxassay_002680_23	euxassay_002680_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 23 24

olfactory cortex marginal layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19 20

spinal cord

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 17 18 weak expression: see section 07 08 11 13 14 15 16 19

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 19 20 21 22

not examined

regional

not examined expression: see section 01 02 03 04 23 24

midbrain

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 18

telencephalon marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5292
Entity Detected:	Acsl6, acyl-CoA synthetase long-chain family member 6 ( MGI:894291)
Sequence:	sense strand is shown >T5292 GCGGCGGCAGAGGCAGGATCTGAGGCTGCGGGGCTGCGGGCCTGGGAAGCAGTGCGGGCCGGGCTGGGCC GGCTCACCGACATGCTGACCTTCTTCTTGGTGTCGGGGGGTTCTCTCTGGCTGTTTGCCGAGATTGCTCT CTCACTTCTGGAGAAGATGCAGACCCAGGAGATCCTGAGGATCCTGCGGCTGCCCGAGCTATCGGACTTG GGCCAGTTTTTCCGCAGCCTCTCAGCTACCACCCTCGTGAGTGTGGGTGCACTGGCTGCCGTCCTTGCCT ACTGGCTCACTCACCGGCCAAAGGCCTTGCAACCACCATGCAACCTCCTGAAGCAGTCGGAAGAAGTGGA GGACGGTGGTGGAGCCCGGCGATCTGTGATTGGGGGTTGCACTCAATTGCTTACCCATTACTATGACGAT GCCCGGACCATGTACCAGGTGTTCCGCCGTGGGCTTAGCATCTCAGGGAATGGACCCTGTCTTGGCTTCC GGAAGCCAGAGCAACCTTACCAGTGGCTGTCCTACCAAGAGGTGGCCAAAAGGGCTGAATTTCTGGGGTC GGGGCTTCTCCAGCACGACTGTAAAGTGGGCACAGAGCAGTTCGTTGGTGTTTTTGCACAAAATCGGCCG GAGTGGATCATTGCCGAGCTGGCCTGCTACACGTATTCCATGGTGGTGGTACCGCTCTACGACACCCTGG GCCCAGGGTCTATCAGCTACATCATCAATACTGCGGACATTTGCACGGTAATCGTCGATAAACCCCACAA GGCAACACTTCTGCTGGAACACGTGGAGAGGAAGGAGACTCCGGGCCTCAAGCTGGTCATCCTCATGGAG CCGTTTGAGGACGCCCTGAGAGAGAGAGGAAAGAAGTGCGGGGTGGACATCAAGTCCATGCAGGCTATAG AGGACTGTGGCCGAGAGAATCATCATGCCCCCGTGCCCCCACGGCCTGATGACCTCTCCATCGTGTGTTT CACAAGTGGTACAACAGGGAACCCCAAAGGTGCAATGCTCACCCACGGGAACGTGGTGGCCGATTTCTCG GGCTTTCTGAAAGTGACAGAGAGTCAGTGGGCCCCGACCTGTGCGGACGTCCACTTTTCCTACTTGCCTT TAGCACACATGTTTGAGCGCATGGTGCAGTCTGTTGTCTACTGCCACGGAGGGCGCGTGGGCTTCTTCCA GGGAGACATCCGCCTCCTCTCAGATGACATGAAGGCTCTCCGCCCTACCATCTTCCCTGTGGTCCCACGG CTGCTGAATCGGATGTATGACAAGATCTTCCACCAGGCAGACACCTCACTAAAGCGCTGGCTCCTGGAGT TTGCAGCAAAGCGCAAGCAGGCAGAGGTCCGGAGCGGAATCATCAGAAACAATAGTATCTGGGATGAACT CTTCTTTAATAAGATTCAGGCCAGTCTTGGTGGGCATGTGAGGATGATTGTCACTGGAGCAGCCCCCGCG TCACCAACGGTTCTGGGATTCCTACGAGCAGCTCTGGGGTGCCAGGTCTATGAAGGTTATGGGCAAACTG AATGCACAGCTGGGTGTACCTTCACAACGCCAGGGGACTGGACATCAGGGCATGTAGGGGCACCTCTGCC CTGCAACCACATCAAGCTGGTCGATGCAGAGGAACTCAACTACTGGACCTGCAAAGGAGAAGGAGAGATA TGTGTGAAAGGACCAAATGTGTTCAAAGGCTACTTAAAAGACGAGGACAGGACAAAGGAGGCCCTGGACA GCGACGGCTGGCTTCACACTGGAGACATTGGGAAATGGCTGCCGGAGGGAACACTCAAAATCATTGATCG GAAAAAGCACATATTTAAACTTGCTCAGGGGGAATATGTTGCGCCAGAGAAGATCGAGAACATCTACATC CGGAGTGAGCCTGTGGCACAAATCTACGTCCACGGGGACAGCTTAAAGGCCTTTTTGGTTGGCATTGTCG TGCCTGACCCTGAAGTCATGCCTTCCTGGGCTCAGAAGAAAGGAATCGAAGGGACCTATCAGGAACTCTG CATGAAAAAGGAATTGAAGAAAGCCATTCTGGATGACATGGTGATGCTGGGGAAAGAAAGCGGACTGCAT TCTTTTGAACAGGTTAAAGCCATTTACATCCATTGTGACATGTTCTCTGTTCAAAATGGTCTGCTGACAC CAACACTAAAGGCTAAGAGACCGGAGCTGAGAGAGTACTTCAAAAAGCAAATAGAAGAGCTTTACTTAGT CTCCGTGTGAAGCTCCAGGAGGGCCCGTCTCAGTCCGATGAACTTTGCAGCAATATTATAGTTATTCGTG AGAGAAAGGAATCAGAATGCCACAGCTCAAAGTGAACAAGCACCTGATTTTTGCAACTGAGCCTTTCCTG ACCCAAGAAGTCTCTAACAATATTTTCTCTACCATCACCAAGTACACTTTATTTTTATTAAATGATATTG TAGCAAGCTGCTAAAAAAAATTATCGCAAACGGTAATGTAAAAACAAATCAAGACACTTTCTGAAGAACT GTGTACCACTAAAAGTAATATATTCGCAATGTTCACAGAATTCCATTAAACATAAAGAAAAAACATAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5362660 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5362660 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002680 same experiment
	EMAGE:30280 same embryo
	EMAGE:31186 same embryo
	EMAGE:30266 same embryo
	EMAGE:31212 same embryo
	EMAGE:29636 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source