EMAGE mouse gene expression spatial database

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EMAGE:31197

Lypla2 ( MGI:1347000)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000279_01	euxassay_000279_02	euxassay_000279_03	euxassay_000279_04	euxassay_000279_05

euxassay_000279_06	euxassay_000279_07	euxassay_000279_08	euxassay_000279_09	euxassay_000279_10

euxassay_000279_11	euxassay_000279_12	euxassay_000279_13	euxassay_000279_14	euxassay_000279_15

euxassay_000279_16	euxassay_000279_17	euxassay_000279_18	euxassay_000279_19	euxassay_000279_20

euxassay_000279_21	euxassay_000279_22	euxassay_000279_23	euxassay_000279_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 05 06 07 08 17 18 19 20 21 22

tail dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3631
Entity Detected:	Lypla2, ( MGI:1347000)
Sequence:	sense strand is shown >T3631 CTTCCCACCCTATCCTCTTCCCACAGGCCTCTGGGGCAGGTCGCCGAGGCTGGCCAGGCCTTCTTGCTGG CCTTGGCCATCTGGAAGTCAGCAGCGGGGTGGGCTGCTCTCTTATCCTGCGTCCCCAGAGGCGGGCCCCA GCAGCAGTATTGGAGGGGCTGTAGGCGGCGGGAGGAAGGGGCCCAGCTGCTGACCCGCTCACTCAGGACC TCACCCACTAGCCCCGCTTTGGGCCCCCTCCCGTGACCTCAGGGTTTGGCCCGTGGGGCCCTCGTGGGCC CCTCCCCCCATGACTCTGCCCGGATAATCGTCTCTCCTGCCTCGCTGCAGCCGCTTCTGTCACGAATGTG TCCATGGCCCGGGGCCCTTGCTGCTGTGGGCTGCCTGCTCCAGGACAGGCTGGCCAGTGAGGAGGTAGAG TCCCTCGGGGCCTCCCCTCAGCCCCTCCCCGAGAGGGGCTGGACCCTGCCTCCCCATGGCCCTTCCTTGC AGGCCTGGAGCCTGCAGGGCTGGATTGAGGTTCAAGGGCCCCCCCAACTCC
Notes:	The probe template was PCR amplified from IMAGE:3168043 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3168043 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000279 same experiment
	EMAGE:30087 same embryo
	EMAGE:31195 same embryo
	EMAGE:29401 same embryo
	EMAGE:29302 same embryo
	EMAGE:31215 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source