EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31212

Porcn ( MGI:1890212)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002692_01	euxassay_002692_02	euxassay_002692_03	euxassay_002692_04	euxassay_002692_05

euxassay_002692_06	euxassay_002692_07	euxassay_002692_08	euxassay_002692_09	euxassay_002692_10

euxassay_002692_11	euxassay_002692_12	euxassay_002692_13	euxassay_002692_14	euxassay_002692_15

euxassay_002692_16	euxassay_002692_17	euxassay_002692_18	euxassay_002692_19	euxassay_002692_20

euxassay_002692_21	euxassay_002692_22	euxassay_002692_23	euxassay_002692_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 01 02 03 22 23 24

olfactory cortex marginal layer

strong

regional

strong expression: see section 08 09 10 11 14 15 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 17 18 19

cervico-thoracic ganglion

weak

regional

weak expression: see section 10

spinal cord

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 17 18

thoracic ganglion

weak

regional

weak expression: see section 11

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

not examined

regional

not examined expression: see section 01 02 03 22 23 24

midbrain

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 21 22 moderate expression: see section 03 04 05 06 07 08 17 18 19 20

telencephalon marginal layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

cervical ganglion

weak

regional

weak expression: see section 08

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24

hindbrain

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5296
Entity Detected:	Porcn, ( MGI:1890212)
Sequence:	sense strand is shown >T5296 CCACGCGTCCGCGGCCTTGCGGCCTGCGGGGGAGGCTGCCAGGAGGAGGTGGCGACAGTGGCAGCGGTGC GCGTCCCCGGCCCCCAGGACCACGGCTTCCTTTCTGCCAGCTCCATCTGTCCATCCATCTGTGTGGGTCC ACAATGGCCACCTTCAGCCGCCAGGAATTTTTCCAGCAGCTACTGCAGGGCTGTCTCCTGCCTACTGTCC AACAGGGCCTTGACCAGATCTGGCTGCTTCTTACCATCTGCTTCGCCTGCCGCCTCCTTTGGAGGCTGGG GTTACCGTCTTACCTGAAGCATGCAAGCACCGTGGCAGGTGGCTTCTTCAGCCTCTACCACTTCTTCCAG CTGCACATGGTTTGGGTCGTGCTGCTGAGCCTCCTGTGCTACCTCGTGCTGTTCCTCTGCCGACACTCCT CCCACCGAGGCGTCTTCCTCTCCGTCACCATCCTCATCTACCTGCTCATGGGTGAGATGCACATGGTGGA CACCGTGACATGGCACAAGATGCGAGGGGCCCAGATGATCGTGGCCATGAAGGCGGTGTCTCTGGGCTTC GACCTGGACCGGGGCGAGGTGGGTGCAGTGCCCTCACCTGTGGAGTTCATGGGCTACCTCTACTTTGTGG GCACCATCGTCTTTGGGCCCTGGATATCCTTCCACAGCTACCTACAGGCTGTCCAAGGCCGCCCACTGAG CCGCCGATGGCTGAAGAAGGTGGCCCGGAGCCTGGCGCTGGCCCTGCTGTGCCTTGTACTGTCCACTTGT GTGGGCCCCTACCTCTTCCCCTACTTCATCCCCCTCGACGGTGACCGACTCCTTCGCAAGGGCACCATGG TAAGGTGGCTACGAGCCTACGAGAGTGCTGTCTCCTTCCACTTCAGCAACTATTTTGTGGGCTTTCTGTC TGAGGCCACAGCCACATTGGCCGGGGCTGGCTTCACGGAGGAGAAGGACCACCTGGAATGGGACCTGACA GTCTCTAGACCGTTGAATGTGGAGCTGCCCCGGTCCATGGTGGAAGTTGTCACAAGCTGGAACCTGCCCA TGTCTTATTGGTTAAATAATTATGTTTTCAAAAATGCCCTTCGCCTGGGGACCTTCTCTGCTGTGCTGGT CACCTATGCAGCCAGTGCCCTCCTGCATGGCTTCAGTTTCCACCTGGCTGCTGTGCTGCTGTCCCTGGCA TTCATCACTTATGTGGAACATGTCCTCAGAAAGCGCCTGGCTCAGATCCTCAGTGCCTGCATCTTGTCAA AGCGTTGTCTGCCAGACTGTTCACATCGGCATCGCTTGGGCCTGGGGGTACGAGCCTTAAACTTGCTCTT TGGGGCCCTGGCTATCTTCCACCTGTCCTACCTGGGCTCCCTGTTTGATGTCGATGTGGACGACACCACA GAGGAGCAGGGCTACGGCATGGCATACACTGTCCACAAGTGGTCAGAGCTCAGCTGGGCCAGTCACTGGG TCACTTTTGGATGCTGGATCTTCTACCGTCTCATAGGCTGAGGCATATGACCATCATAATCCCCTTCAAT ACCCCTCCTTAGGGTGCCAACTCTGATGGGGGATGAGAAAAGACCCTTTCTCTACTCCTTTTCCCTTATC CATTCTTGACCCCCAACACCCCTCCACACAACATAGGAACACACACAACTTTCTATGCCTGTCAATCCTC ACCCCGCCACCAGGCAGGAAGGGGGTGGTCCTTATTGGGCCAAGGAACGATGGATACTTGGGAAAGCAGA CAGGAGCAGATCCAGGGCCTCTGCGCCTGCCGTCTCCCTTTTTTTATATGCAGTTTGTTATTGTCGAATA AAAGTAGAACTTACACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5362485 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5362485 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002692 same experiment
	EMAGE:30280 same embryo
	EMAGE:31193 same embryo
	EMAGE:31186 same embryo
	EMAGE:30266 same embryo
	EMAGE:29636 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source