EMAGE mouse gene expression spatial database

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EMAGE:31213

Ube2t ( MGI:1914446)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004872_01	euxassay_004872_02	euxassay_004872_03	euxassay_004872_04	euxassay_004872_05

euxassay_004872_06	euxassay_004872_07	euxassay_004872_08	euxassay_004872_09	euxassay_004872_10

euxassay_004872_11	euxassay_004872_12	euxassay_004872_13	euxassay_004872_14	euxassay_004872_15

euxassay_004872_16	euxassay_004872_17	euxassay_004872_18	euxassay_004872_19	euxassay_004872_20

euxassay_004872_21	euxassay_004872_22	euxassay_004872_23	euxassay_004872_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 17 18 19 20

thymus primordium

moderate

regional

moderate expression: see section 10 11 14 15 weak expression: see section 12 13

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 14 15 17

vibrissa

weak

regional

weak expression: see section 08 09 10 22 23

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 15 16 17 18 19 20 moderate expression: see section 02 03 04 05 06 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 moderate expression: see section 07 08 15

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 19 20

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 12 13 17 18

testis

moderate

regional

moderate expression: see section 05 06 17 18 19

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6544
Entity Detected:	Ube2t, ( MGI:1914446)
Sequence:	sense strand is shown >T6544 GGGGCTGCTGACGGGTGCTGCGAGTGCCACCTGAGGGAACGAGCCCGAGGTCACGGAGAGTCTGGGGAAG GAGTCCCGTTTCTACCCGAGCGGCCCTGGGAGGAACACGGCGGGGAGAGCGAACCGCCCTGGGCGCGCCA AGGCTACCTCCAGCATCTGCCACCTACCCAGTACACCCCCGCCAGACCTCAGTGTGGAACAGTGAACTGT TTCTGGTTCCTTTCACTTGAGATCATGCAGAGAGCCTCACGCCTGAAGAAGGAACTGCACATGCTAGCCA TCGAACCTCCCCCGGGCATCACGTGCTGGCAGGAAAAGGATCAAGTGGCTGATTTGCGAGCTCAAATACT AGGTGGAGCTAATACACCTTATGAGAAAGGTGTTTTCACACTGGAAGTTATCATTCCTGAGAGGTACCCA TTTGAACCTCCACAGGTCCGATTTCTAACTCCAATCTATCATCCAAACATTGATTCTAGTGGAAGGATTT GTCTAGATATTCTCAAATTGCCACCAAAGGGTGCATGGAGACCATCCCTCAACATTGCCACTGTATTGAC CTCTATTCAGCTGCTCATGGCAGAACCCAATCCTGATGACCCACTAATGGCTGACATTTCTTCAGAATTT AAATATAACAAGATAGCTTTTCTCAAGAAAGCCAAGCAGTGGACAGAGGCTCATGCAAGACAGAAACAAA AGGCTGATGAAGAGGAGCTAGGCACCTCATCAGAAGTTGGTGACTCTGAGGAAAGCCATTCCACACAGAA AAGGAAGGCCAGGCCATTAGGAGGAATGGAAAAAAAGTTTTCCCCTGACGTTCAGAGAGTCTATCCTGGT CCCTCTTAGTTACGGCAGTCTGTGTCAAGGTGGTCTCTTTTGCTTGCTTCTTTTAAATGTTTTCCTTTGT ATTTGATGACAAATGTTTGTATCCTTACAAAAGACCTTGTATGTTTACGAATTCTGCCATGCAGCGATTC TTTAAGGATAGTTTGTTTTATTTATCTTGTACATATGTATTTAGAAAACTTTTAAACTTGAAAAATAAAT AATACTTAATGTTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591557 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591557 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004872 same experiment
	EMAGE:30229 same embryo
	EMAGE:31241 same embryo
	EMAGE:30227 same embryo
	EMAGE:31216 same embryo
	EMAGE:29347 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source