EMAGE mouse gene expression spatial database

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EMAGE:31223

Rasgef1b RasGEF domain family, member 1B ( MGI:2443755)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000486_01	euxassay_000486_02	euxassay_000486_03	euxassay_000486_04	euxassay_000486_05

euxassay_000486_06	euxassay_000486_07	euxassay_000486_08	euxassay_000486_09	euxassay_000486_10

euxassay_000486_11	euxassay_000486_12	euxassay_000486_13	euxassay_000486_14	euxassay_000486_15

euxassay_000486_16	euxassay_000486_17	euxassay_000486_18	euxassay_000486_19	euxassay_000486_20

euxassay_000486_21	euxassay_000486_22	euxassay_000486_23	euxassay_000486_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 11 12 13 14 15

not examined

homogeneous

not examined expression: see section 01 02 03 04 05 06 07 08 09 10

medulla oblongata basal plate

weak

homogeneous

weak expression: see section 11 14

cerebral cortex mantle layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24

vibrissa

weak

regional

weak expression: see section 03 04 05 18 19 20 21 22

not examined

homogeneous

not examined expression: see section 04 05 06

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 16 17 18 19

midbrain mantle layer

moderate

regional

moderate expression: see section 13 14 15 16 17 18

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 06 07 11 12 13 14 15 18 19

olfactory cortex marginal layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 14 15 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 18 19 20 21 22 weak expression: see section 06 07

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T159
Entity Detected:	Rasgef1b, RasGEF domain family, member 1B ( MGI:2443755)
Sequence:	sense strand is shown >T159 TTTCCCACAGCTGACAGATGGACTTGGATCTCTTTTGAGACTGAAATCGTCACTGAAGACACTTGAGGGA GAATCTTCTGAAAGCCCCGGCTCTGCGCATGATTCATCTTCTGGAATTTCCATGTGAATCACAGCTCTGC ACCTGGATGGACTTTTGTTTGTGATATATTTTTTTTTTGGCCTGAACATTTGCTGCTAATGGAACTTACA CAGAGTGCGGCTCTATGGAAGCCCAAGTTTCTTTGTTAATTTAAATGAAAAAGGGAGAGGGAGGGGAGGG GAAACCCCTCCCACCCACCAAGTTTGGATCCTGGACCTCAGCTTGAACAGTATTCCAGTGAGGGCAAAGA TTGGGTGGGGTTCTACTGCAGACATCCCCTTACCCAGTGCGGTATTGTCAACACCCCTCCCCCGTCCCCC TCTTCCTCCAAGGCTTTGAGTTGGCTGCTGGCTAGCAAACTCCTTCTTACCCATTAAGTTATTTAATATA ATAATGAAGCGCAACACTGTGGTAGGAAA
Notes:	The probe template was PCR amplified from IMAGE:2631273 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2631273 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000486 same experiment
	EMAGE:31271 same embryo
	EMAGE:30316 same embryo
	EMAGE:30289 same embryo
	EMAGE:30286 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source