Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30316

Csen (Csen)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30316 EMAGE:30316 EMAGE:30316 EMAGE:30316 EMAGE:30316
euxassay_000471_01 euxassay_000471_02 euxassay_000471_03 euxassay_000471_04 euxassay_000471_05
EMAGE:30316 EMAGE:30316 EMAGE:30316 EMAGE:30316 EMAGE:30316
euxassay_000471_06 euxassay_000471_07 euxassay_000471_08 euxassay_000471_09 euxassay_000471_10
EMAGE:30316 EMAGE:30316 EMAGE:30316 EMAGE:30316 EMAGE:30316
euxassay_000471_11 euxassay_000471_12 euxassay_000471_13 euxassay_000471_14 euxassay_000471_15
EMAGE:30316 EMAGE:30316 EMAGE:30316 EMAGE:30316 EMAGE:30316
euxassay_000471_16 euxassay_000471_17 euxassay_000471_18 euxassay_000471_19 euxassay_000471_20
EMAGE:30316 EMAGE:30316 EMAGE:30316
euxassay_000471_21 euxassay_000471_22 euxassay_000471_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3816
Entity Detected:Csen, (Csen)
Sequence:sense strand is shown

>T3816
TGGCCTCGAGCCAGATTCGGACGAGGGTTTCCTGGAGGAACATCCCCACAAAAGTACATTCCATCATCTG
AAGCCCGGTCTCTGCTCAGGCCTGCCTCTGAAAGTCCACGTGTGTTCCCCAGAAGGCCAGCCCCAAGATA
AGGGAGGTCCTTAGAGGAAGGACAGGGTGACAACACCCCTATACACAGGTGGACCCCCCCCCCCTCTGAG
GGCTGTACTGACCCCATCTCCATCCTGACCGGGGCCTTCCTTTACCCGATCTACAGACCACCAGTTCTCC
CTGGCTCAGGGACCCCCTGTCCCCCAGTCTGACTCTTCCCATCGAGGTCCCTGTCTTGTGAAAAGCCAAG
GCCACGGGAAAAGGCCACCACTCTAACCTGCTGCATCCCTTAGCCTCTGGCTGCACGCCCAACCTGGAGG
GGTCTGTCCCCTTTGCAGGGACACAGACTGGCCGCATGTCCGCATGGCAGAAGCGTCTCCCTTGGGTGCA
GCCTGGAAGGGTGGTTCCTGTCTCAGCGCCCACCAATATTCAGTCCTATATATTTTAA
Notes:The probe template was PCR amplified from IMAGE:406125 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:406125 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000471 same experiment
 EMAGE:31271 same embryo
 EMAGE:30289 same embryo
 EMAGE:31223 same embryo
 EMAGE:30286 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS