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EMAGE:31241

Cst3 cystatin C ( MGI:102519)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31241 EMAGE:31241 EMAGE:31241 EMAGE:31241 EMAGE:31241
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 15 16
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 11 13 14
olfactory cortex ventricular layer
moderate moderate
homogeneousmoderate expression: see section 11 12 16 17 18
ventral grey horn
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17
pons mantle layer
moderate moderate
regionalmoderate expression: see section 07 17 18
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 13 14
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6575
Entity Detected:Cst3, cystatin C ( MGI:102519)
Sequence:sense strand is shown

>T6575
GTCTTGTTGGAGCTTTATCCCTTTGTCCTAGCCAACCATGGCCAGCCCGCTGCGCTCCTTGCTGTTCCTG
CTGGCCGTCCTGGCCGTGGCCTGGGCGGCGACCCCAAAACAAGGCCCGCGAATGTTGGGAGCCCCGGAGG
AGGCAGATGCGAATGAGGAAGGCGTGCGGCGAGCGTTGGACTTCGCTGTGAGCGAGTACAACAAGGGCAG
CAACGATGCGTACCACAGCCGCGCCATACAGGTGGTGAGAGCTCGTAAGCAGCTCGTGGCTGGAGTGAAC
TATTTTTTGGATGTGGAGATGGGCCGAACTACATGTACCAAGTCCCAGACAAATTTGACTGACTGTCCTT
TCCATGACCAGCCCCATCTGATGAGGAAGGCACTCTGCTCCTTCCAGATCTACAGCGTGCCCTGGAAAGG
CACACACTCCCTGACAAAATTCAGCTGCAAAAATGCCTAAGGGCTGAGTCTAGAAGGATCATGCAGATTG
TTCCTTACTTGTGCTCCTTCCCTATAGTGTTTCATCTCGCAGAAGGGTGCTCCGGCTCTGGAGGGCACCG
CCAGTGTGTTTGCACCAGGAGACAGTAAAGGAGCTGCTGCAGGCAGGTTCTGCACATCTGAACAGCTGTC
CCCTGGCTCCACTCTTCTTGCAGTACCTATCATGCCTTGCTCAATTAAAAAAAAAACTCCTTTGGCAAAA
AAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3593373 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593373 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004853 same experiment
 EMAGE:30229 same embryo
 EMAGE:31213 same embryo
 EMAGE:30227 same embryo
 EMAGE:31216 same embryo
 EMAGE:29347 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS