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EMAGE:31306

0610010K14Rik ( MGI:1915609)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31306 EMAGE:31306 EMAGE:31306 EMAGE:31306 EMAGE:31306
euxassay_010029_01 euxassay_010029_02 euxassay_010029_03 euxassay_010029_04 euxassay_010029_05
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euxassay_010029_06 euxassay_010029_07 euxassay_010029_08 euxassay_010029_09 euxassay_010029_10
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euxassay_010029_11 euxassay_010029_12 euxassay_010029_13 euxassay_010029_14 euxassay_010029_15
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euxassay_010029_16 euxassay_010029_17 euxassay_010029_18 euxassay_010029_19 euxassay_010029_20
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euxassay_010029_21 euxassay_010029_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
tibia
weak weak
regionalweak expression: see section 21 22
femur
weak weak
regionalweak expression: see section 02 03 17 18 19 20 21
maxilla
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 05 11 12 14
mandible
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 02 03 04 05 11 12 13 14 15 17 18 20
fibula
weak weak
regionalweak expression: see section 21 22
rib
moderate moderate
regionalmoderate expression: see section 16 17 18 19 20 21 22 weak expression: see section 01 02 03 04 05 06
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 20 21 22 weak expression: see section 02 03 19
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31801
Entity Detected:0610010K14Rik, ( MGI:1915609)
Sequence:sense strand is shown

>T31801
CATGACGTCAGCGTCCACCAAGGTTGGAGAGATCTTCTCCGCGGCCGGCGCCGCCTTCACGAAGCTCGGG
GAGTTGACGATGCAGCTGCATCCAGTCTCGGACTCTTCCCCTGCCGGTGCCAAGTGGACGGAGACGGAGA
TAGAGATGCTGAGGGCTGCTGTGAAGCGCTTTGGGGACGATCTTAATCACATCAGCTGTGTCATCAAGGA
ACGGACAGTGGCTCAGATAAAGACCACTGTGAAGCGAAAAGTGTATGAAGACTCCGGCATCCCCCTTCCA
GCTGAGTCACCCAAGAAAGGACCCAAGAAGATGACGTCCGGTGTCCTCTCACCTCCAAACGCCCCTCCAC
CCAGCAGCTCCAGTGTCCCTGAAGCTGGGGTTCCTCCCATAAAGAAACAGAAGGCTGACAGCCTGACCCT
GGACTCTGGCCTCCTCACCACCTCCGCTGATGCTCCTCTCCTCTCCTGCTGAGCCTTCCACCTCTGACCC
TCATTGTTCACCCTGGACCTGTGGATCACCTGGGACTCTAAGCAAGGACTGCCTGAGAGAGGGTCAGAGG
GCTGCTGGAGCCATCCACCTCGTTCCTGATAACCATGTGCCCTCG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5325264), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 63689. Forward Primer - name:063689_F_IRAV98_e12_0610010K14Rik, sequence:CATGACGTCAGCGTCCAC; Reverse Primer - name:063689_R_SP6_IRAV98_e12_0610010K14Rik, sequence:GCGAGGGCACATGGTTA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_010029 same experiment
 EMAGE:31207 same embryo
 EMAGE:31253 same embryo
 EMAGE:31255 same embryo
 EMAGE:29436 same embryo
 EMAGE:29426 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS